LOCUS       sequence-202050-        4557 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  mitochondrial calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Hirabayashi Y, Kwon SK, Paek H, Pernice WM, Paul MA, Lee J, Erfani 
            P, Raczkowski A, Petrey DS, Pon LA, Polleux F
  TITLE     ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in 
            mammalian neurons.
  JOURNAL   Science. 2017 Nov 3;358(6363):623-630. doi: 10.1126/science.aan6009.
  PUBMED    29097544
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "623-630"
COMMENT     Sequence Label: pCAG mito-RCaMP1h
FEATURES             Location/Qualifiers
     source          1..4557
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..108
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             145..162
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             166..198
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(202..210,211..225)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      232..241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             253..309
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(1555..1574)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1620..1675
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1674..1693)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2066..2082)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2066..2082)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2079..2101)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2090..2106
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2114..2120,2121..2138,2139..2144))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2159..2180
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2239..2434
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2285..2420
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2440..2574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2477..2496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2531..2550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2642..2659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2813..3401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2893..2912)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3572..4363,4364..4432))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4195..4214
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4433..4537)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgtctcatc attttggcaa agaattgctc gagatgtccg tcctgacgcc gctgctgctg
       61 cggggcttga caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg
      121 ggggatccac cggtcatggg ttctcatcat catcatcatc atggtatggc tagcatgact
      181 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatct cgccaccatg
      241 gtcgactcat cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg
      301 ctgagctcag cgatcaacac cgaaatgatg tacccagcgg atggtggtct gcgtggttac
      361 actcacatgg cgctgaaagt tgatggcggc ggtcacctgt cctgttcttt cgtgaccacc
      421 taccgctcca aaaagactgt cggcaacatt aagatgcctg ccattcatta cgtcagccac
      481 cgtctggagc gcctggagga gagcgataac gaaatgtttg tcgtacagcg tgaacacgca
      541 gttgccaagt ttgtgggcct gggtggtggc ggcggtaccg gagggagcat gaactccctg
      601 atcaaggaga acatgcgtat gaaagtggtt ctggaaggct ccgtaaacgg ccaccagttc
      661 aaatgcactg gtgaaggcga aggcaacccg tatatgggca cccagactat gcgtatcaaa
      721 gtgatcgagg gtggtccgct gccgtttgcg ttcgacatcc tggcgacgtc ctttatgtat
      781 ggctcccgta ccttcatcaa atatccgaaa ggcatcccgg atttctttaa gcagtccttc
      841 ccggaaggtt ttacctggga acgtgtgacc cgttacgaag acggcggcgt aattaccgtt
      901 atgcaagaca cgtctctgga ggatggctgc ctggtgtatc acgtgcaggt tcgcggtgtt
      961 aacttcccga gcaatggtgc tgtaatgcaa aagaaaacca aaggttggga gcctacggac
     1021 tcccaactga ctgaagagca gatcgcagaa tttaaagagg ctttctccct atttgacaag
     1081 gacggggatg ggacaataac aaccaaggag ctggggacgg tgatgcggtc tctggggcag
     1141 aaccccacag aagcagagct gcaggacatg atcaatgaag tagatgccga cggtgacggc
     1201 acaatcgact tccctgagtt cctgattatg atggcaagaa aaatgaaata cacagacagt
     1261 gaagaagaaa ttagagaagc gttcggcgtg tttgataagg atggcaatgg ctacatcagt
     1321 gcagcagagc ttcgccacgt gatgacaaac cttggagaga agttaacaga tgaagaggtt
     1381 gatgaaatga tcagggaagc agacagcgat ggggatggtc aggtaaacta cgaagagttt
     1441 gtacaaatga tgacagcgaa gtaagcggcc gcaattcact cctcaggtgc aggctgccta
     1501 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
     1561 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
     1621 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
     1681 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
     1741 ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa gaggtcatca
     1801 gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct tgacttgagg
     1861 ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc taaaattttc
     1921 cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag tcatagctgt
     1981 ccctcttctc ttatggagat ccctcgacct gcaccgtcga ccagctggtc gacggtgcac
     2041 cgtcgaccag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2101 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2161 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2221 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     2281 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     2341 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     2401 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     2461 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2521 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     2581 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3241 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgc
//
