LOCUS       sequence-202052-        4464 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  cytosolic calcium sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Hirabayashi Y, Kwon SK, Paek H, Pernice WM, Paul MA, Lee J, Erfani 
            P, Raczkowski A, Petrey DS, Pon LA, Polleux F
  TITLE     ER-mitochondria tethering by PDZD8 regulates Ca(2+) dynamics in 
            mammalian neurons.
  JOURNAL   Science. 2017 Nov 3;358(6363):623-630. doi: 10.1126/science.aan6009.
  PUBMED    29097544
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2017-11-3- 3"; volume: "358"; issue: 
            "6363"; pages: "623-630"
COMMENT     Sequence Label: pCAG cyto-RCaMP1h
FEATURES             Location/Qualifiers
     source          1..4464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..68
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..104
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(108..116,117..131)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      138..147
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             159..215
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(1461..1480)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1526..1581
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1580..1599)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1972..1988)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1972..1988)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1985..2007)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1996..2012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2020..2026,2027..2044,2045..2050))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2065..2086
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2145..2340
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      2191..2326
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2346..2480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2383..2402)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2437..2456
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2548..2565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2719..3307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2799..2818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3478..4269,4270..4338))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4101..4120
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4339..4443)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcattttggc aaagaattgc tcgagctcaa gcttcgaatt catgggttct catcatcatc
       61 atcatcatgg tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg
      121 atgacgataa ggatctcgcc accatggtcg actcatcacg tcgtaagtgg aataagacag
      181 gtcacgcagt cagagctata ggtcggctga gctcagcgat caacaccgaa atgatgtacc
      241 cagcggatgg tggtctgcgt ggttacactc acatggcgct gaaagttgat ggcggcggtc
      301 acctgtcctg ttctttcgtg accacctacc gctccaaaaa gactgtcggc aacattaaga
      361 tgcctgccat tcattacgtc agccaccgtc tggagcgcct ggaggagagc gataacgaaa
      421 tgtttgtcgt acagcgtgaa cacgcagttg ccaagtttgt gggcctgggt ggtggcggcg
      481 gtaccggagg gagcatgaac tccctgatca aggagaacat gcgtatgaaa gtggttctgg
      541 aaggctccgt aaacggccac cagttcaaat gcactggtga aggcgaaggc aacccgtata
      601 tgggcaccca gactatgcgt atcaaagtga tcgagggtgg tccgctgccg tttgcgttcg
      661 acatcctggc gacgtccttt atgtatggct cccgtacctt catcaaatat ccgaaaggca
      721 tcccggattt ctttaagcag tccttcccgg aaggttttac ctgggaacgt gtgacccgtt
      781 acgaagacgg cggcgtaatt accgttatgc aagacacgtc tctggaggat ggctgcctgg
      841 tgtatcacgt gcaggttcgc ggtgttaact tcccgagcaa tggtgctgta atgcaaaaga
      901 aaaccaaagg ttgggagcct acggactccc aactgactga agagcagatc gcagaattta
      961 aagaggcttt ctccctattt gacaaggacg gggatgggac aataacaacc aaggagctgg
     1021 ggacggtgat gcggtctctg gggcagaacc ccacagaagc agagctgcag gacatgatca
     1081 atgaagtaga tgccgacggt gacggcacaa tcgacttccc tgagttcctg attatgatgg
     1141 caagaaaaat gaaatacaca gacagtgaag aagaaattag agaagcgttc ggcgtgtttg
     1201 ataaggatgg caatggctac atcagtgcag cagagcttcg ccacgtgatg acaaaccttg
     1261 gagagaagtt aacagatgaa gaggttgatg aaatgatcag ggaagcagac agcgatgggg
     1321 atggtcaggt aaactacgaa gagtttgtac aaatgatgac agcgaagtaa gcggccgcaa
     1381 ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
     1441 gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag
     1501 ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt
     1561 tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat
     1621 cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac
     1681 aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta
     1741 ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt
     1801 ttttctttaa catccctaaa attttcctta catgttttac tagccagatt tttcctcctc
     1861 tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcac
     1921 cgtcgaccag ctggtcgacg gtgcaccgtc gaccagcttg gcgtaatcat ggtcatagct
     1981 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2041 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2101 actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg atccgcatct caattagtca
     2161 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     2221 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
     2281 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     2341 aagctaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     2401 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     2461 tatcttatca tgtctggatc cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2521 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2581 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2641 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2701 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2761 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2821 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2881 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2941 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     3001 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     3061 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     3121 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     3181 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     3241 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     3301 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3361 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3421 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3481 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3541 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3601 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3661 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3721 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3781 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3841 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3901 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3961 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     4021 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     4081 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     4141 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     4201 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     4261 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     4321 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4381 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4441 gcgcacattt ccccgaaaag tgcc
//
