LOCUS       sequence-202371-       10303 bp    DNA     circular SYN 14-APR-2025
DEFINITION  GFP vector for cloning in guides.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10303)
  AUTHORS   Weintraub AS, Li CH, Zamudio AV, Sigova AA, Hannett NM, Day DS, 
            Abraham BJ, Cohen MA, Nabet B, Buckley DL, Guo YE, Hnisz D, Jaenisch
            R, Bradner JE, Gray NS, Young RA
  TITLE     YY1 Is a Structural Regulator of Enhancer-Promoter Loops.
  JOURNAL   Cell. 2017 Dec 14;171(7):1573-1588.e28. doi: 
            10.1016/j.cell.2017.11.008. Epub 2017 Dec 7.
  PUBMED    29224777
REFERENCE   2  (bases 1 to 10303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.11.008"; journalName: "Cell"; date: 
            "2017-12-14- 14"; volume: "171"; issue: "7"; pages: "1573-1588.e28"
COMMENT     Sequence Label: pAW12.lentiguide.GFP
FEATURES             Location/Qualifiers
     source          1..10303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        86..1264
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          317..1255
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1212..1232
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1271..1280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1277..1279,1280..1282,1283..1996)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1277..1279,1280..1282,1283..1993)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1322..1343)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1583..1602)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1930..1951
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2012..2600
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2065..2085)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2483..2494)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2672..2905
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2983..3104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3020..3039)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3074..3093
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3144..3279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3299..3318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3300..3318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3328..3345)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3328..3344)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3337..3359)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3486..3941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3573..3592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3783..3804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3967..4071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4072..4140,4141..4932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4290..4309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5103..5691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5592..5611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5845..5862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5979..6000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6015..6020,6021..6038,6039..6045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6053..6069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6058..6080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6077..6093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6077..6093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6112..6132
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6114..6132
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6160..6386
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6387..6567
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6614..6739
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7232..7465
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7650..7694
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7872..8120
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7872..7892
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     8043..8062
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        10002..10077
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10133..10250
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
       61 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
      121 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgatccggtg cctagagaag
      181 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
      241 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
      301 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
      361 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
      421 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
      481 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
      541 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
      601 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
      661 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
      721 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
      781 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
      841 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
      901 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
      961 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     1021 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     1081 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     1141 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     1201 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     1261 gtgacgtacg gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     1321 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     1381 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     1441 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     1501 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     1561 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     1621 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     1681 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     1741 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     1801 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     1861 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     1921 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     1981 cgagctgtac aagtaaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     2041 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2101 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2161 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2221 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2281 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2341 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2401 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2461 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2521 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2581 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     2641 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2701 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     2761 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2821 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     2881 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     2941 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3001 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3061 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3121 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3181 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3241 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     3301 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3361 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3421 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3481 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3901 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     3961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4081 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4141 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4201 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4261 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4321 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4381 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4441 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4501 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4561 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4621 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4681 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4741 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4801 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4861 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4921 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4981 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5041 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5101 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5161 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5221 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5281 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5341 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5401 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5461 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5521 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5581 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5641 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5701 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5761 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5821 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5881 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5941 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6001 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6061 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6121 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     6181 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6241 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     6301 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     6361 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6421 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6481 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6541 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6601 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     6661 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     6721 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     6781 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     6841 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     6901 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     6961 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7021 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7081 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     7141 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7201 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7261 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7321 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7381 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7441 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7501 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7561 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7621 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     7681 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     7741 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     7801 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     7861 ggggacccag agagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     7921 ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta gtacaaaata
     7981 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     8041 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     8101 tgtggaaagg acgaaacacc ggagacggtt gtaaatgagc acacaaaata cacatgctaa
     8161 aatattatat tctatgacct ttataaaatc aaccaaaatc ttctttttaa taactttagt
     8221 atcaataatt agaattttta tgttcctttt tgcaaacttt taataaaaat gagcaaaata
     8281 aaaaaacgct agttttagta actcgcgttg ttttcttcac ctttaataat agctactcca
     8341 ccacttgttc ctaagcggtc agctcctgct tcaatcattt tttgagcatc ttcaaatgtt
     8401 ctaactccac cagctgcttt aactaaagca ttgtctttaa caactgactt cattagttta
     8461 acatcttcaa atgttgcacc tgattttgaa aatcctgttg atgttttaac aaattctaat
     8521 ccagcttcaa cagctatttc acaagctttc atgatttctt cttttgttaa taaacaattt
     8581 tccataatac atttaacaac atgtgatcca gctgcttttt ttacagcttt catgtcttct
     8641 aaaactaatt cataattttt gtcttttaat gcaccaatat ttaataccat atcaatttct
     8701 gttgcaccat ctttaattgc ttcagaaact tcgaatgctt ttgtagctgt tgtgcatgca
     8761 cctagaggaa aacctacaac atttgttatt cctacatttg tgccttttaa taattcttta
     8821 caatagcttg ttcaatatga attaacacaa actgttgcaa aatcaaattc aattgcttca
     8881 tcacataatt gtttaatttc agctttcgta gcatcttgtt ttaataatgt gtgatctata
     8941 tatttgttta gtttcatttt ttctcctata tattcatttt taattttaat tctttaataa
     9001 tttcgtctac tttaacttta gcgttttgaa cagattcacc aacacctata aaataaattt
     9061 ttagtttagg ttcagttcca cttgggcgaa cagcaaatca tgacttatct tctaaataaa
     9121 attttagtaa gtcttgtcct ggcatattat acattccatc gatgtagtct tcaacattaa
     9181 caactttaag tccagcaatt tgagttaagg gtgttgctct caatgatttc attaatggtt
     9241 caatttttaa tttcttttct tctggtttaa aattcaagtt taaagtgaaa gtgtaatatg
     9301 cacccatttc tttaaataaa tcttctaaat agtctactaa tgttttattt tgttttttat
     9361 aaaatcaagc agcctctgct attaatatag aagcttgtat tccatcttta tctctagctg
     9421 agtcatcaat tacatatcca taactttctt cataagcaaa aacaaaattt aatccgttat
     9481 cttcttcttt agcaatttct ctacccattc atttaaatcc agttaaagtt tttacaatat
     9541 taactccata tttttcatga gcgattctat cacccaaatc acttgttaca aaacttgaat
     9601 atagagccgg attttttgga atgctattta agcgttttag atttgataat tttcaatcaa
     9661 ttaaaattgg tcctgtttga tttccatcta atcttacaaa atgaccatca tgttttattg
     9721 ccattccaaa tctgtcagca tctgggtcat tcataataat aatatctgca tcatgtttaa
     9781 taccatattc aagcggtatt tttcatgcag gatcaaattc tggatttgga tttacaacat
     9841 ttttaaatgt ttcatcttca aatgcatgct cttcaacctc aataacgtta tatcctgatt
     9901 cacgtaatat ttttggggta aatttagttc ctgttccatt aactgcgcta aaaataattt
     9961 ttaaatcttt tttagcttct tgctcttttt tgtacgtctc tgttttagag ctagaaatag
    10021 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
    10081 tttaagcttg gcgtaactag atcttgagac aaatggcagt attcatccac aattttaaaa
    10141 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    10201 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    10261 acagggacag cagagatcca ctttggcgcc ggctcgaggg ggc
//
