LOCUS       sequence-202463-       12844 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for 
            Drosophila) in Drosophila germline tissues using Gal4 drivers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Alberti C, Manzenreither RA, Sowemimo I, Burkard TR, Wang J, 
            Mahofsky K, Ameres SL, Cochella L
  TITLE     Cell-type specific sequencing of microRNAs from complex animal 
            tissues.
  JOURNAL   Nat Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610.
  PUBMED    29481550
REFERENCE   2  (bases 1 to 12844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2018 Feb 26. pii: nmeth.4610. doi: 10.1038/nmeth.4610."
COMMENT     Sequence Label: pUASp-FMW-attB-AthHen1
FEATURES             Location/Qualifiers
     source          1..12844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      627..636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             636..701
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      717..726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             726..755
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             765..794
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             804..833
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             843..872
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             882..911
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             945..974
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    987..1011
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3877..3901)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    5380..5449
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(5668..5686)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5707..5723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5707..5723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5720..5742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5731..5747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5755..5761,5762..5779,5780..5785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5800..5821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5938..5955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6109..6697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6189..6208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6868..7659,7660..7728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7491..7510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7729..7833)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7859..8314)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7996..8017)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8208..8227
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8441..8463
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8455..8472
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8456..8472
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8479..8498
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8479..8497
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(9001..9072,9474..9747,9822..10476,10538..10853,
                     11057..11188,11259..11873)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    12648..12681
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gaatgttctc tctcttctct tctctctctc tttctcgagg tcatcaagct taggcctcca
       61 aggcggagta ctgtcctccg ggctggcgga gtactgtcct ccggcaaggc ggagtactgt
      121 cctccgggct ggcggagtac tgtcctccgg caaggcggag tactgtcctc cgggctggcg
      181 gagtactgtc ctccggcaag gcggagtact gtcctccggg ctggcggagt actgtcctcc
      241 ggcaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca agggtcgagt
      301 cgatagccga agcttaccga agtatacact taaattcagt gcacgtttgc ttgttgagag
      361 gaaaggttgt gtgcggacga attttttttt gaaaaccggt gatagagcct gaaccagaaa
      421 agataaaaga aggctatacc agtgggagta cacaaacaga gtaagtttga atagtaaaaa
      481 aaatcattta tgtaaacaat aacgtgactg tgcgttaggt cctgttcatt gatcgaggcc
      541 tgtctagaga agcttgttcg aatctcgagt gcgcgcttcc ggaggtatac acctaggcgg
      601 taccactgca gtgaattcgg agctccgcca ccatggacta caaagaccat gacggtgatt
      661 ataaagatca tgacatcgat tacaaggatg acgatgacaa gcaccggttg agctccgcca
      721 ccatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt
      781 ctgaagagga cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa
      841 tggagcaaaa gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg
      901 aagaggactt gaatgaaatg gagagcttgg gcgacctcac catggagcaa aagctcattt
      961 ctgaagagga cttgaatcac cggtatacaa gtttgtacaa aaaagcaggc tccgcggccg
     1021 cccccttcac catggcgggt ggtggcaagc acaccccgac tccaaaggct attatccatc
     1081 aaaagtttgg cgctaaggcc tcgtatacag tggaggaagt tcatgactcg tcgcagtcgg
     1141 gctgcctggg cctggccatt ccgcagaagg gtccgtgcct ctaccggtgt cacctgcagc
     1201 tgccagaatt ctccgtggtg agcaatgtat tcaaaaagaa gaaggatagt gagcagagtg
     1261 ctgctgagct ggccttggat aaactgggta ttcgacccca gaacgacgat ttgacagtgg
     1321 atgaggcgcg cgatgagatc gtgggcagga tcaaatatat attcagtgac gagttcctga
     1381 gtgccgagca cccgttgggc gcacacttgc gcgcagccct gcgtcgcgat ggcgaacgtt
     1441 gcggctcggt tccggttagt gtgattgcta ccgtggacgc caaaattaac agccgctgta
     1501 agataatcaa tccctccgtg gagagtgacc cgttcctggc catttcgtac gtgatgaaag
     1561 cagcagctaa gctggccgac tacatagtgg cctcgccaca cggcctccgt cgcaaaaacg
     1621 cgtacccgtc cgagatagtc gaggcgttgg cgacgcacgt aagcgattcg ctccattccc
     1681 gggaggtggc cgctgtttac attccgtgca tcgatgagga ggttgtagag ttggatacgc
     1741 tctacatcag ctcgaatcgc cattacctgg attcgatcgc cgagcgactc ggtttgaagg
     1801 acggcaatca ggttatgatt agtcgcatgt ttggcaaggc gtcgtgtggt tccgagtgtc
     1861 gtctgtactc ggagatccca aagaagtatc tggacaattc cagcgatgcc agcggtactt
     1921 ccaacgagga ttccagccat atcgtcaaga gccgcaacgc tcgcgcctcg tacatctgtg
     1981 gtcaggatat ccatggtgac gccatcctcg ccagcgtagg ataccgatgg aagtcggacg
     2041 acctggacta cgatgatgtg acagtcaaca gtttctacag gatatgctgc ggcatgtccc
     2101 cgaatggtat atacaaaata agcaggcagg ccgtgatcgc ggcgcagctg cccttcgcct
     2161 ttactacgaa gagcaattgg cgcggtccgt tgccccgtga gatcctgggt ctgttctgcc
     2221 accagcaccg actggcggaa ccaattctgt ccagcagtac cgcgccggtg aagagtttgt
     2281 ccgacatctt tcgcagtcac aagaagttga aggtaagcgg tgttgacgac gcaaacgaga
     2341 acttgtcccg ccagaaggaa gatacgcccg gactgggtca cggcttccga tgtgaggtta
     2401 agatttttac caagagtcag gacctggtac tcgaatgctc cccacgtaaa ttctacgaga
     2461 aggagaacga tgccatccag aacgcctcct tgaaggcctt gctctggttc agcaagttct
     2521 ttgccgacct ggacgttgac ggcgagcaaa gttgcgacac tgatgatgat caggatacga
     2581 agagcagtag cccaaacgta ttcgccgccc cccccatctt gcagaaggag cactcctcgg
     2641 agagcaagaa caccaatgtg ctcagtgcgg aaaagcgggt ccagtccatc accaatggca
     2701 gtgtcgtgag tatttgctac tccctcagcc tggccgtcga tcccgagtat tcgtccgatg
     2761 gcgaaagccc cagggaggat aatgaaagta acgaggagat ggaaagtgaa tacagcgcca
     2821 actgtgagtc gagtgtggag ctgattgagt cgaacgagga gatcgagttc gaggtcggca
     2881 ccggcagcat gaacccgcac attgagagcg aggtgaccca aatgaccgtt ggtgagtacg
     2941 ccagctttcg catgacaccg ccagacgcgg cagaggcact gatcctggca gtgggcagcg
     3001 acaccgtgcg aatccgctcc ctgctgagcg agaggccatg cctcaattac aacatcctgc
     3061 tgctgggcgt taagggaccc agtgaggagc gcatggaggc cgccttcttc aagccccctc
     3121 tctcgaagca acgagtggag tatgccctga agcatattcg agagtcgagt gcatcgacgc
     3181 tggtggattt tggctgtggc tccggaagtc tgttggatag tctcctggac tacccaacaa
     3241 gcctgcaaac gattataggc gtggacatct cgcctaaggg cctcgcccgt gccgcgaaga
     3301 tgctgcacgt taaattgaac aaggaggcgt gcaatgttaa gagtgctact ctgtacgatg
     3361 gaagcattct cgagttcgac agccgcctgc acgatgttga cattggcact tgtttggaag
     3421 tgattgaaca catggaagag gatcaagcct gcgagtttgg tgaaaaggtg ttgagtttgt
     3481 tccacccgaa gctgctcata gtgagcaccc ccaattatga atttaacacg atactgcagc
     3541 gctcgacgcc cgagacgcag gaggagaaca actccgagcc acagctcccg aaattcagga
     3601 accatgacca taaattcgag tggacacggg agcagtttaa tcaatgggct tcgaagctgg
     3661 gcaagcgcca caactactcc gtggagttca gcggtgtcgg aggcagtggc gaggtggaac
     3721 cgggctttgc cagccagatc gctatattcc ggcgtgaagc tagctcggtc gagaatgtcg
     3781 cagaaagtag catgcaaccc tacaaagtga tttgggaatg gaagaaggag gacgtcgaaa
     3841 aaaagaagac tgacttgtaa aagggtgggc gcgccgaccc agctttcttg tacaaagtgg
     3901 tgacgtaagc tagagtcgac ctcgaacgtt aacgttaacg taacgttaac tcgaggagct
     3961 tgataacatt atacctaaac ccatggtcaa gagtaaacat ttctgccttt gaagttgaga
     4021 acacaattaa gcatcccctg gttaaacctg acattcatac ttgttaatag cgccataaac
     4081 atagcaccaa tttcgaagaa atcagttaaa agcaattagc aattagcaat tagcaataac
     4141 tctgctgact tcaaaacgag aagagttgca agtatttgta aggcacagtt tatagaccac
     4201 cgacggctca ttagggctcg tcatgtaact aagcgcggtg aaacccaatt gaacatatag
     4261 tggaattatt attatcaatg gggaagattt aaccctcagg tagcaaagta atttaattgc
     4321 aaatagagag tcctaagact aaataatata tttaaaaatc tggccctttg accttgcttg
     4381 tcaggtgcat ttgggttcaa tcgtaagttg cttctatata aacactttcc ccatccccgc
     4441 aataatgaag aataccgcag aataaagaga gatttgcaac aaaaaataaa ggcattgcga
     4501 aaacttttta tgggggatca ttacactcgg gcctacggtt acaattccca gccacttaag
     4561 cgacaagttt ggccaacaat ccatctaata gctaatagcg caatcactgg taatcgcaag
     4621 agtatatagg caatagaacc catggatttg accaaaggta accgagacaa tggagaagca
     4681 agaggatttc aaactgaaca cccacagtgc tgtgtactac cactggcgcg tttgggagct
     4741 cactggcctg atgcgtcctc cgggcgtttc aagcctgctt tacgtggtat actccattac
     4801 ggtcaacttg gtggtcaccg tgctgtttcc cttgagcttg ctggccaggc tgctgttcac
     4861 caccaacatg gccggattgt gcgagaacct gaccataact attaccgata ttgtggccaa
     4921 tttgaagttt gcgaatgtgt acatggtgag gaagcagctc catgagattc gctctctcct
     4981 aaggctcatg gacgctagag cccggctggt gggcgatccc gaggagattt ctgccttgag
     5041 gaaggaagtg aatatcgcac agggcacttt ccgcaccttt gccagtattt tcgtatttgg
     5101 cactactttg agttgcgtcc gcgtggtcgt tcgcccggat cgagagctcc tgtatccggc
     5161 ctggttcggc gttgactgga tgcactccac cagaaactat gtgctcatca atatctacca
     5221 gctcttcggc ttgatagtgc aggctataca gaactgcgct agtgactcct atccgcctgc
     5281 gtttctctgc ctgctcacgg gtcatatgcg tgctttggag ctgagggtgc ggcggattgg
     5341 ctgcagagat ccactagtgt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg
     5401 ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat
     5461 gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc
     5521 ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc
     5581 ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc
     5641 gacactagtt ctagccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     5701 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5761 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5821 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5881 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     5941 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6001 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6061 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6121 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6181 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6241 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6301 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6361 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6421 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6481 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6541 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6601 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6661 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6721 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6781 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6841 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6901 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     6961 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7021 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7081 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7141 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7201 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7261 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7321 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7381 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7441 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7501 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7561 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7621 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7681 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7741 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7801 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     7861 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     7921 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     7981 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     8041 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     8101 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     8161 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     8221 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     8281 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     8341 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     8401 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     8461 acgacggcca gtgaattgta atacgactca ctatagggcg aattgggtac gtaccgggcc
     8521 cctagtatgt atgtaagtta ataaaaccca tttttgcgga aagtagataa aaaaaacatt
     8581 tttttttttt actgcactgg atatcattga acttatctga tcagttttaa atttacttcg
     8641 atccaagggt atttgatgta ccaggttctt tcgattacct ctcactcaaa atgacattcc
     8701 actcaaagtc agcgctgttt gcctccttct ctgtccacag aaatatcgcc gtctctttcg
     8761 ccgctgcgtc cgctatctct ttcgccaccg tttgtagcgt tacgtagcgt caatgtccgc
     8821 cttcagttgc attttgtcag cggtttcgtg acgaagctcc aagcggttta cgccatcaat
     8881 taaacacaaa gtgctgtgcc aaaactcctc tcgcttctta tttttgtttg ttttttgagt
     8941 gattggggtg gtgattggtt ttgggtgggt aagcagggga aagtgtgaaa aatcccggca
     9001 atgggccaag aggatcagga gctattaatt cgcggaggca gcaaacaccc atctgccgag
     9061 catctgaaca atgtgagtag tacatgtgca tacatcttaa gttcacttga tctataggaa
     9121 ctgcgattgc aacatcaaat tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa
     9181 ccgcaattgc acaaacaaat agtgacacga aacagattat tctggtagct gttctcgcta
     9241 tataagacaa tttttgagat catatcatga tcaagacatc taaaggcatt cattttcgac
     9301 tatattcttt tttacaaaaa atataacaac cagatatttt aagctgatcc tagatgcaca
     9361 aaaaataaat aaaagtataa acctacttcg taggatactt cggggtactt tttgttcggg
     9421 gttagatgag cataacgctt gtagttgata tttgagatcc cctatcattg cagggtgaca
     9481 gcggagcggc ttcgcagagc tgcattaacc agggcttcgg gcaggccaaa aactacggca
     9541 cgctccggcc acccagtccg ccggaggact ccggttcagg gagcggccaa ctagccgaga
     9601 acctcaccta tgcctggcac aatatggaca tctttggggc ggtcaatcag ccgggctccg
     9661 gatggcggca gctggtcaac cggacacgcg gactattctg caacgagcga cacataccgg
     9721 cgcccaggaa acatttgctc aagaacggtg agtttctatt cgcagtcggc tgatctgtgt
     9781 gaaatcttaa taaagggtcc aattaccaat ttgaaactca gtttgcggcg tggcctatcc
     9841 gggcgaactt ttggccgtga tgggcagttc cggtgccgga aagacgaccc tgctgaatgc
     9901 ccttgccttt cgatcgccgc agggcatcca agtatcgcca tccgggatgc gactgctcaa
     9961 tggccaacct gtggacgcca aggagatgca ggccaggtgc gcctatgtcc agcaggatga
    10021 cctctttatc ggctccctaa cggccaggga acacctgatt ttccaagcca tggtgcggat
    10081 gccacgacat ctgacctatc ggcagcgagt ggcccgcgtg gatcaggtga tccaggagct
    10141 ttcgctcagc aaatgtcagc acacgatcat cggtgtgccc ggcagggtga aaggtctgtc
    10201 cggcggagaa aggaagcgtc tggcattcgc ctccgaggct ctaaccgatc cgccgcttct
    10261 gatctgcgat gagcccacct ccggactgga ctcctttacc gcccacagcg tcgtccaggt
    10321 gctgaagaag ctgtcgcaga agggcaagac cgtcatcctg accattcatc agccgtcttc
    10381 cgagctgttt gagctctttg acaagatcct tctgatggcc gagggcaggg tagctttctt
    10441 gggcactccc agcgaagccg tcgacttctt ttcctagtga gttcgatgtg tttattaagg
    10501 gtatctagta ttacataaca tctcaactcc tatccagcgt gggtgcccag tgtcctacca
    10561 actacaatcc ggcggacttt tacgtacagg tgttggccgt tgtgcccgga cgggagatcg
    10621 agtcccgtga tcggatcgcc aagatatgcg acaattttgc cattagcaaa gtagcccggg
    10681 atatggagca gttgttggcc accaaaaatc tggagaagcc actggagcag ccggagaatg
    10741 ggtacaccta caaggccacc tggttcatgc agttccgggc ggtcctgtgg cgatcctggc
    10801 tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact tattcagaca acggtgagtg
    10861 gttccagtgg aaacaaatga tataacgctt acaattcttg gaaacaaatt cgctagattt
    10921 tagatagaat tgcctgattc cacacccttc ttagtttttt tcaatgagat gtatagttta
    10981 tagttttgca gaagataaat aaatttcatt taactcgcga atattaatga gatgcgagta
    11041 acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact
    11101 cacgcaagtg ggtgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac
    11161 ctttcaaaac gtctttgcca cgataaatgt aagtcatgtt tagaatacat ttgcatttca
    11221 ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag
    11281 tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa
    11341 cgattgccga attgccgctt tttctcacag tgccactggt cttcacggcg attgcctatc
    11401 cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc
    11461 tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct
    11521 cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct
    11581 tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc
    11641 gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta
    11701 gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta
    11761 acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct
    11821 tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat
    11881 atatccgaaa taactgcttg tttttttttt ttaccattat taccatcgtg tttactgttt
    11941 attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct
    12001 atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct
    12061 aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa
    12121 acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca
    12181 cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac
    12241 acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt
    12301 cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt
    12361 atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc
    12421 taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt
    12481 tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa
    12541 aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa
    12601 tacatttaat ttagaaaatg cttggatttc actggaacta ggctagcata acttcgtata
    12661 atgtatgcta tacgaagtta tgctagcgga tctgttcaat gatatccagt gcagtaaaaa
    12721 aaaaaaatgt tttttttatc tactttccgc aaaaatgggt tttattaact tacatacata
    12781 ctagaattgg ccgctctagc cccccctcga atgttctctc tcttctcttc tctctctctt
    12841 tctc
//
