LOCUS       sequence-202899-        3117 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Phaeodactylum U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Graff van Creveld S, Ben-Dor S, Mizrachi A, Alcolombri U, Hopes A, 
            Mock T, Rosenwasser S, Vardi A
  TITLE     Biochemical Characterization of a Novel Redox-Regulated Metacaspase 
            in a Marine Diatom.
  JOURNAL   Front Microbiol. 2021 Sep 8;12:688199. doi: 
            10.3389/fmicb.2021.688199. eCollection 2021.
  PUBMED    34566902
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-09-8"; volume: "12:688199. doi"; pages: " 
            10.3389/fmicb.2021.688199. eCollection 2021"
COMMENT     Sequence Label: pCR8/GW:PtU6
FEATURES             Location/Qualifiers
     source          1..3117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(315..414)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(430..447)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(430..446)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(439..461)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      510..596
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      688..715
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(874..891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1045..1633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1125..1144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1726..2517)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2948..2964
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2948..2964
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2969..2988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2969..2987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3005..3104
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tggtctcagg aggttggctc ggaagttggt gttgacggtg agctggaaat tggttgtcgg
       61 tcactgctag cgagaagaaa acggaggaca gaaggaagtg aaactcggtt cgttctcgac
      121 agcctcactg tcaatatgct cattttcaat ccttagcgct tttaatgtcg aattgacggt
      181 aaattgaata ggatctataa tatctacaag gtactttgac acgccaagta ttcattgtta
      241 gtcaacaata ttttagagct ttataaggtc aaaaaacacc ttcaaagtcg agtgagacct
      301 aagggcgaat tcggagcctg cttttttgta caaagttggc attataaaaa agcattgctc
      361 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttggggccc
      421 gagcttaaga ctggccgtcg ttttacaacg tcgtgactgg gaaaacatcc atgctagcgt
      481 taacgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcggaagact
      541 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      601 cgggagcgga tttgaacgtt gtgaagcaac ggcccggagg gtggcgggca ggacgcccgc
      661 cataaactgc caggcatcaa actaagcaga aggccatcct gacggatggc ctttttgcgt
      721 ttctacaaac tcttcctggc tagcggtacg cgtattaatt gcgttgcgct cactgcccgc
      781 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1021 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct
     1261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     1501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     1681 cgcgtaactc acgttaaggg attttggtca tgggtggctc gagggttatt tgccgactac
     1741 cttggtgatc tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc
     1801 caagcgatct tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata
     1861 ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc
     1921 ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc
     1981 ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg
     2041 ttcaggaacc ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc
     2101 tcttgctttt gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc
     2161 tgcaagaatg tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg
     2221 ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct
     2281 ctctccaggg gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc
     2341 atcaagcctt acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc
     2401 atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat
     2461 gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat
     2521 gtttaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
     2581 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac
     2641 cccaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg
     2701 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagcttacg
     2761 aaccgaacag gcttatgtcc actgggttcg tgccttcatc cgtttccacg gtgtgcgtca
     2821 cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg tctagaggag
     2881 gcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag acacgggcca
     2941 gagctgccag gaaacagcta tgaccatgta atacgactca ctatagggga tatcagctgg
     3001 atggcaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
     3061 gcaattattt tttataatgc caactttgta caagaaagct gggtcgaatt cgccctt
//
