LOCUS       sequence-203238-       10584 bp    DNA     circular SYN 15-APR-2025
DEFINITION  MSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 
            and Flag-tagged each).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10584)
  AUTHORS   Roe JS, Hwang CI, Somerville TDD, Milazzo JP, Lee EJ, Da Silva B, 
            Maiorino L, Tiriac H, Young CM, Miyabayashi K, Filippini D, 
            Creighton B, Burkhart RA, Buscaglia JM, Kim EJ, Grem JL, Lazenby AJ,
            Grunkemeyer JA, Hollingsworth MA, Grandgenett PM, Egeblad M, Park Y,
            Tuveson DA, Vakoc CR
  TITLE     Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
  JOURNAL   Cell. 2017 Aug 24;170(5):875-888.e20. doi: 
            10.1016/j.cell.2017.07.007. Epub 2017 Jul 27.
  PUBMED    28757253
REFERENCE   2  (bases 1 to 10584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.07.007"; journalName: "Cell"; date: 
            "2017-08-24- 24"; volume: "170"; issue: "5"; pages: "875-888.e20"
COMMENT     Sequence Label: MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5
FEATURES             Location/Qualifiers
     source          1..10584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1419..1460
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2877..2933
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2934..2999
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        4227..4726
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4262..4285)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4659..4678
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4740..5543
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4803..4822)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5413..5432
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    5612..6179
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5629..6181
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5773..5790)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6000..6019
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6193..6195,6196..6198,6199..6912)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6238..6259)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6499..6518)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6846..6867
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6979..7493
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7655..7671
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7679..7685,7686..7703,7704..7709))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7724..7745
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7862..7879)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8033..8621)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8113..8132)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8792..9583,9584..9652))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9415..9434
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9653..9757)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9825..9843
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9881..9903)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10003..10022
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10216..10238
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10366..10385
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tcaccatggg taagcctatc cctaaccctc
     1441 tcctcggtct cgattctacg ttagggactg tgaagatgga agggcatgag agcaacgact
     1501 ggaacagcta ctacgcggac acgcaggagg cctactcctc tgtccctgtc agcaacatga
     1561 actccggcct gggctctatg aactccatga acacctacat gaccatgaac accatgacca
     1621 cgagcggcaa catgaccccg gcttccttca acatgtccta cgccaacacg ggcttagggg
     1681 ccggcctgag tcccggtgct gtggctggca tgccaggggc ctctgcaggc gccatgaaca
     1741 gcatgactgc ggcgggcgtc acggccatgg gtacggcgct gagcccggga ggcatgggct
     1801 ccatgggcgc gcagcccgcc acctccatga acggcctggg tccctacgcc gccgccatga
     1861 acccgtgcat gagtcccatg gcgtacgcgc cgtccaacct gggccgcagc cgcgcggggg
     1921 gcggcggcga cgccaagaca ttcaagcgca gctaccctca cgccaagccg ccttactcct
     1981 acatctcgct catcacgatg gccatccagc aggcgcccag caagatgctc acgctgagcg
     2041 agatctacca gtggatcatg gacctcttcc cctattaccg ccagaaccag cagcgctggc
     2101 agaactccat ccgccactcg ctgtccttca acgattgttt cgtcaaggtg gcacgatccc
     2161 cggacaagcc aggcaagggc tcctactgga cgctgcaccc ggactccggc aacatgttcg
     2221 agaacggctg ctacttgcgc cgccaaaagc gcttcaagtg tgagaagcag ccgggggccg
     2281 gaggtgggag tgggggcggc ggctccaaag ggggcccaga aagtcgcaag gacccctcag
     2341 gcccggggaa ccccagcgcc gagtcacccc ttcaccgggg tgtgcacgga aaggctagcc
     2401 agctagaggg cgcgccggcc ccagggcccg ccgccagccc ccagactctg gaccacagcg
     2461 gggccacggc gacagggggc gcttcggagt tgaagtctcc agcgtcttca tctgcgcccc
     2521 ccataagctc cgggccaggg gcgctagcat ctgtaccccc ctctcacccg gctcacggcc
     2581 tggcacccca cgaatctcag ctgcatctga aaggggatcc ccactactcc tttaatcacc
     2641 ccttctccat caacaacctc atgtcctcct ccgagcaaca gcacaagctg gacttcaagg
     2701 catacgagca ggcgctgcag tactctcctt atggcgctac cttgcccgcc agtctgcccc
     2761 ttggcagcgc ctcagtggcc acgaggagcc ccatcgagcc ctcagccctg gagccagcct
     2821 actaccaagg tgtgtattcc agacccgtgc taaatacttc cctcgagggc agcggcgcca
     2881 ccaacttcag cctgctgaag caggccggcg acgtggagga gaaccccggc cccgactaca
     2941 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaagt
     3001 accaaagctt ggcgttagcc cagagccccg gccagggcac ttacgccgac tccggagcct
     3061 ttctgcactc ttcgggaacc ggctccccgg tgttcgtggc acccactcgc atgccctcca
     3121 tgctgccgta cctgccttcg tgtgagccgg gctcgcaggc tcccgcgctc gccgcgcact
     3181 ccagctggac acaagcagtt gccgctgatt cctcagcctt cggctccgga agcccgcacc
     3241 cgccagccgc acacccaccg ggagccacca ccttcccgtt cgctcacagc cctccggggt
     3301 cgggcagcgg cggcagcgcg ggggtccggg acggcggcgc cttccagggc gcgctgctgg
     3361 ctcgcgaaca gtacccgacc ccgctcggga gacccatggg cgcctcgtac cccacaacct
     3421 acccagcata catgagttcc gacgtagccc cttcgtggac ctccggagcc ttcgacagca
     3481 gcatcctgca cggtctgcaa gctcgccctg gtggcctccc tggccgcagg accagcttcg
     3541 tacctgactt cttggaggag ttccctggtg agggccggga gtgcgtcaac tgtggagccc
     3601 tgtctacacc actgtggcgc agggacggca ccggacacta tctatgcaat gcctgcggcc
     3661 tctatcacaa gatgaacggg gtcaaccgac cgctagtgag gccccagaag cgtctgtcct
     3721 catcccgaag atccggccta tgctgctcca actgccatac tgccaccacc accctctgga
     3781 ggcgcaactc ggagggcgaa ccagtgtgca acgcctgtgg cctctacatg aagctccatg
     3841 gggtgccaag gccactggca atgaaaaagg agagtatcca gacaagaaaa cggaagccaa
     3901 agaaccctgc caaaatcaag ggctcctcag gatccacagc aaacaccaca gcctcttctc
     3961 ccactctcct caactctgag agctcagcca ccactttgaa ggcagagtcc agtctggcat
     4021 ctccagtgtg tgctgggccc actatcacct cccaggcctc cagcccagcg gacgaatccc
     4081 tggcctccag ccatttggag ttcaagttcg aacctgaaga ctttgccttc acctcctcgt
     4141 ccatgagccc ccaggctggt ctcagtgggg tcctacgtca ggagacctgg tgtgctctgg
     4201 ccttggccta gagatctaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
     4261 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
     4321 cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc
     4381 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
     4441 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
     4501 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
     4561 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gnnggctcag
     4621 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
     4681 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagccaata
     4741 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     4801 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     4861 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     4921 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     4981 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5041 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5101 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5161 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     5221 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     5281 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     5341 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     5401 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     5461 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     5521 gccttcttga cgagttcttc tgaggggatc ccgcccgccc cacgacccgc agcgcccgac
     5581 cgaaaggagc gcacgacccc atgcatccaa ttccgccccc cccccctaac gttactggcc
     5641 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     5701 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     5761 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     5821 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     5881 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     5941 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     6001 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     6061 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     6121 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     6181 atggccacaa ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     6241 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtctggcga gggcgagggc
     6301 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     6361 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     6421 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     6481 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     6541 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     6601 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     6661 aagcagaaga acggcatcaa ggcgaacttc aagatccgcc acaacatcga ggacggcagc
     6721 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     6781 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     6841 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     6901 ctgtacaagt aagtcgacct gcagccaagc ttatcgataa aataaaagat tttatttagt
     6961 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
     7021 acgccatttt gcaaggcatg gaaaatacat aactgagaat agagaagttc agatcaaggt
     7081 taggaacaga gagacagcag aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     7141 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tcccgccctc agcagtttct
     7201 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     7261 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     7321 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgatagact gcgtcgcccg
     7381 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     7441 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catgggtaac
     7501 agtttcttga agttggagaa caacattctg agggtaggag tcgaatatta agtaatcctg
     7561 actcaattag ccactgtttt gaatccacat actccaatac tcctgaaata gttcattatg
     7621 gacagcgcag aaagagctgg ggagaattgt gaaattgtta tccgctcaca attccacaca
     7681 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9781 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     9841 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     9901 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     9961 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
    10021 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    10081 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    10141 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    10201 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg
    10261 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
    10321 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
    10381 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
    10441 gaggcgatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc tagttttgac
    10501 tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt
    10561 atttagtctc cagaaaaagg gggg
//
