LOCUS       sequence-203409-        4007 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Entry vector for CELF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Meyer C, Garzia A, Mazzola M, Gerstberger S, Molina H, Tuschl T
  TITLE     The TIA1 RNA-Binding Protein Family Regulates EIF2AK2-Mediated 
            Stress Response and Cell Cycle Progression.
  JOURNAL   Mol Cell. 2018 Feb 15;69(4):622-635.e6. doi: 
            10.1016/j.molcel.2018.01.011.
  PUBMED    29429924
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2018.01"; journalName: "Mol Cell"; date: 
            "2018-02-15- 15"; volume: "69"; issue: "4"; pages: "622-635.e6"
COMMENT     Sequence Label: pENTR4_CELF1
FEATURES             Location/Qualifiers
     source          1..4007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2132..2231)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2248..2267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2249..2267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2272..2288)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2272..2288)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2390..2409)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2401..3210
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2468..2487)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3357..3945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3846..3865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atgaacggca ccctggacca cccagaccaa ccagatcttg atgctatcaa
      721 gatgtttgtg ggccaggttc caaggacctg gtctgaaaag gacttgcggg aactcttcga
      781 acagtatggt gctgtgtatg aaatcaacgt cctaagggat aggagccaaa acccgcctca
      841 gagcaaaggg tgctgttttg ttacatttta cacccgtaaa gctgcattag aagctcagaa
      901 tgctcttcac aacatgaaag tcctcccagg gatgcatcac cctatacaga tgaaacctgc
      961 tgacagtgag aagaacaatg cagtggaaga caggaagctg tttattggta tgatttccaa
     1021 gaagtgcact gaaaatgaca tccgagtcat gttctcttcg tttggacaga ttgaagaatg
     1081 ccggatattg cggggacctg atggcctgag ccgaggttgt gcatttgtga cttttacaac
     1141 aagagccatg gcacagacgg ctatcaaggc aatgcaccaa gcacagacca tggagggttg
     1201 ctcatcaccc atggtggtaa aatttgctga tacacagaag gacaaagaac agaagagaat
     1261 ggcccagcag ctccagcagc agatgcagca aatcagcgca gcatctgtgt ggggaaacct
     1321 tgctggtcta aatactcttg gaccccagta tttagcactt tatttgcagc tccttcagca
     1381 gactgcctcc tctgggaacc tcaacaccct gagcagcctc cacccaatgg gagggttgaa
     1441 tgcaatgcag ttacagaatt tggctgcact agctgctgca gctagtgcag ctcagaacac
     1501 accaagtggt accaatgctc tcactacatc cagcagtccc ctcagcgtgc tcactagttc
     1561 agggtcctca cctagctcta gcagcagtaa ttctgtcaac cccatagcct cacttggagc
     1621 cctgcagaca ttagctggag caacggctgg cctcaatgtt ggctctttgg caggaatggc
     1681 tgctttaaat ggtggcctgg gcagcagtgg cctttccaat ggcaccggga gcaccatgga
     1741 ggccctcact caggcctact cgggtatcca gcaatatgct gctgctgcgc tccccactct
     1801 gtacaaccag aatcttctga cacagcagag tattggtgct gctggaagcc agaaggaagg
     1861 tccagaggga gccaacctgt tcatctacca cctgccccag gagtttggtg atcaggacct
     1921 gctgcagatg tttatgccct ttgggaatgt cgtgtctgcc aaggttttca tagacaagca
     1981 gacaaacctg agcaagtgtt ttggttttgt aagttacgac aatcctgttt cggcccaagc
     2041 tgccatccag tccatgaacg gctttcagat tggcatgaag cggcttaaag tgcagctcaa
     2101 acgttcgaag aatgacagca agccctactg aacccagctt tcttgtacaa agttggcatt
     2161 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2221 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2281 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2341 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2401 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2461 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2521 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2581 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2641 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2701 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2761 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2821 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     2881 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     2941 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     3001 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     3061 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     3121 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3181 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3241 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3301 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3481 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     3541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     3601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     3661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     3721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     3781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     3841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     3901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     3961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtt
//
