LOCUS       sequence-203599-        9086 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  heat shock inducible transgenesis, PACT is a centriol marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9086)
  AUTHORS   Strzyz PJ, Lee HO, Sidhaye J, Weber IP, Leung LC, Norden C
  TITLE     Interkinetic nuclear migration is centrosome independent and ensures
            apical cell division to maintain tissue integrity.
  JOURNAL   Dev Cell. 2015 Jan 26;32(2):203-19. doi: 
            10.1016/j.devcel.2014.12.001. Epub 2015 Jan 15.
  PUBMED    25600237
REFERENCE   2  (bases 1 to 9086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.12.001"; journalName: "Dev Cell"; date: 
            "2015-01-26- 26"; volume: "32"; issue: "2"; pages: "203-19"
COMMENT     Sequence Label: HS-GFP-PACT
FEATURES             Location/Qualifiers
     source          1..9086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1623..1639
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1659..1679
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3199..3223
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3226..3235
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3232..3234,3235..3237,3238..3948)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3232..3234,3235..3237,3238..3948)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3277..3298)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3538..3557)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3885..3906
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3949..4014
                     /label=MCS
                     /note="multiple cloning site"
     protein_bind    complement(4028..4052)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4556..4576)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4584..4600)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    4616..4750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4640..4659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4694..4713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5422..5438)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5422..5438)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5475..5495)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5475..5493)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5514..5530)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5527..5549)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5538..5554
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5562..5568,5569..5586,5587..5592))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5607..5628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5745..5762)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5916..6504)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5996..6015)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6675..7466,7467..7535))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7298..7317
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7536..7640)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7666..8121)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7803..7824)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8015..8034
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8248..8270
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8263..8279
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8289..8308
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8289..8307
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
       61 gattgattga ttgattgatt ggtagtcaaa ataagaaata atttccacag attcattaca
      121 gaaatgatta aatgcataca taaaaaactn gggggggggg gggatacaac aacacactta
      181 agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa tagctatatt ttacatctcc
      241 tttttttgct gtcttttaat tagccttgtt ttgctgttat cttgattgaa actgtaactt
      301 cttcacctgc ttcttttctt tgtaggtttt gccagcccag aaacaggcat ggctgtgcaa
      361 ggccagagca ccatgcacaa tgcgctgcat gtcttcatga acggctcaat gtcctcagtc
      421 cagggctcag ccaacgaccc catcttcctc cttcaccatg ctttcattga caggtaacaa
      481 acacgtcatg acattagact gcacagtttt tgacaaagtt catacaatct gttgtttata
      541 gctgctacaa ttagtgaagt ttgtgaatgt acttggatga gcagcgaaag atcaattgag
      601 atcaattgtt agagtttggt tgccctgcag agcaaagaac aaaaaataat ctggtggctt
      661 tactgcgtga ggttattatt ggtggaatag aaacacaaaa cataattgca tttatttgtt
      721 taatttttta tcttatctta actttcatct tgcatatttg tttcttacat catttctagc
      781 atctttgagc gctggctaag aactcatcag cctccccggt ccatctaccc acgtaccaat
      841 gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct ttataggaat
      901 ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct gttggaccca
      961 ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat atctgttcag
     1021 acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt cttgacagag
     1081 gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag ggaaaatttt
     1141 actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt aaaaaagtac
     1201 tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa agaaaactag
     1261 agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata caaggtttta
     1321 tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa tctaatgcca
     1381 gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc tgattctcaa
     1441 aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca gtgctgaaaa
     1501 gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag aggctgcaaa
     1561 tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga tgggccctcg
     1621 agcaggaaac agctatgacc atgattacgc caagctatca actttgtata gaaaagttgc
     1681 ttcaggggtg tcgcttggtt atttccaaaa atcaaattaa ttttattaaa ctattagaac
     1741 gagcatgttt tgtctatatg ctacagaaga taaaaaataa taggagttaa cagttataaa
     1801 acaacacact ttgtttctat tgattgttga ccacactggg gtctcattaa gttagattaa
     1861 agacacacta actgggtcaa aagcagcaga ttgatttcat agcaccaggg taaactttct
     1921 aacactttta cggcaatcat atacattaaa attaaataca gaccacgact gaacaaggag
     1981 gatgatctcc aatattaaac aaagagactt gtgcctattt ctctgagggt aaacatgacc
     2041 tctcaagtta gcaagttggt tttaacacta caaaaatagt taagactgca atcccagaat
     2101 aaagtattgg ttttaaccaa tcaatatagt acagtaaaca tccatttgtt ttgttgaaac
     2161 gttaaacaaa tctgaccaaa gctattagct tatataaaac aggtttgcct tctatgtagc
     2221 tgaaaacacc acaggcccga ttttgctact gtgtaaaaca tttcagcaag atttttttat
     2281 tgcatttttt tttactgaat cgttcaaaca ttttatcatt ttagtttgtt cattcattgc
     2341 aactggaaaa acaacacatc acacaaccgc acatatttca gcaataagta caataaaaca
     2401 ctcaaataaa aaaaacattt taaatctctt tgtatttttg accgctgttt cgcgtaattt
     2461 cacggtaaaa ctctggaaat ctccactaca ttcctctcag cggctcctct caatgacagc
     2521 tgaagaagtg acgcggctgc ctgctgtgtt ttgattggtc gaattcactg gaggcttcca
     2581 gaacagtgta gagtctgaac gggtgcgcgc tctgctgtat ttaaagggcg aaagagagac
     2641 cgcagagaaa ctcaaccgaa gagaagcgac ttgacaaaga agaaaagagc agcctgacag
     2701 gactttttcc ccgacgaggt gtttattcgc tctatttaag aatctactgt aaggtaagtc
     2761 tcaatatatt gtactctatt ggctaatcag aattatatag agattatatg tacttaatgt
     2821 caaaaaatca actttgtata tgtaatcttt ttacatgtgg actgcctatg ttcatcttat
     2881 tttaggtcta ctagaaaatt atatttcccg ttttcacaat aaggattttt aaaaaaagca
     2941 atgaacagac gggcatttac tttatgttgc tgacattatt ttatatgagc ataataacca
     3001 taaatactag caaatgtcct aaatgaattt gtgttaatgt tgtctacaaa agaaaattag
     3061 cgttttactt gtacaactaa taataacttg gttattaaga gaatttcact tgttgactag
     3121 aaaaatcctt tcataatgaa acaattgcac cataaattgt ataaatataa aattaattct
     3181 aattgttttt ttttcctgcc aagtttgtac aaaaaagcag gctgggccac catggtgagc
     3241 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     3301 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     3361 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     3421 accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     3481 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     3541 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     3601 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     3661 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     3721 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     3781 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3841 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3901 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagtc cggactcaga
     3961 tctcgagctc aagcttcgaa ttctgcagtc gacggtaccg cgggcccggg atccaccgga
     4021 tctagacacc cagctttctt gtacaaagtg gctgccaaca ttgaagccat cattgcctct
     4081 gaaaaagaag tatggaacag agaaaaattg actctccaga aatctttgaa aagggcagag
     4141 gctgaagtat acaaactgaa agctgaacta agaaatgact ctttacttca aactctgagc
     4201 cctgattctg aacatgtcac tttaaagaga atttatggta aatacttgag ggcagaaagt
     4261 tttcgaaagg ctctcattta ccagaagaaa tacctgctgc tgttactggg tgggttccag
     4321 gaatgtgaag atgccacctt ggccctgctt gcccggatgg gggggcagcc agctttcacg
     4381 gatctagagg tgatcaccaa tcgcccaaag ggcttcacca ggtttcggtc ggccgtcaga
     4441 gtatccattg caatttccag aatgaaattt ttggttcgac ggtggcatcg agtcacaggt
     4501 tctgtttcca tcaatattaa cagagatggc tttggactga atcaaggtgc ataggcaact
     4561 ttattataca tagttgataa ttcactggcc gtcgttttac ggtaccatcg atgatgatcc
     4621 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4681 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4741 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4801 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     4861 agatcagatc tgcgaagata cggccacggg tgctcttgat cctgtggctg attttggact
     4921 gtgctgctcg cagctgctga tgaatcacat acttcctcca ttttcttcca ctgattgact
     4981 gttataattt ccctaatttc caggtcaagg tgctgtgcat tgtggtaata gatgtgacat
     5041 gacgtcactt ccaaaggacc aatgaacatg tctgaccaat ttcatataat gtgaaaacga
     5101 ttttcatagg cagaataaat aacatttaaa ttaaactggg catcagcgca attcaattgg
     5161 tttggtaata gcaagggaaa atagaatgaa gtgatctcca aaaaataagt actttttgac
     5221 tgtaaataaa attgtaagga gtaaaaagta cttttttttc taaaaaaatg taattaagta
     5281 aaagtaaaag tattgatttt taattgtact caagtaaagt aaaaatcccc aaaaataata
     5341 cttaagtaca gtaatcaagt aaaattactc aagtacttta cacctctggt tcttgacccc
     5401 ctaccttcag caagcccagc agatccacta gttctagagc ggccgccacc gcggtggagc
     5461 tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     5521 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     5581 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5641 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     5701 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     5761 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     5821 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     5881 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5941 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6001 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6061 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6121 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6181 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6241 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     6301 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     6361 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     6421 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     6481 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     6541 cagtggaacg aaaactcacg ttaaggcatt ttggtcatga gattatcaaa aaggatcttc
     6601 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     6661 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     6721 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     6781 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     6841 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6901 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6961 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7021 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7081 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7141 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7201 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7261 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     7321 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     7381 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     7441 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     7501 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     7561 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     7621 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
     7681 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     7741 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     7801 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     7861 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     7921 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     7981 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     8041 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     8101 atgcgccgct acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc
     8161 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     8221 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     8281 agcgcgcgta atacgactca ctatagggcg aattgggtac caaatagtag gaattaccca
     8341 cctgtacaag tgctgaaaac ttggatgaat aagcccgttt gccattttct actgctattt
     8401 taaatctttt ctgtatttgt ctgcatttgt ctttaccctt gaataaatgt tttagttgtt
     8461 ttttttcaat tatgactgtg tttaacagac attattacaa taatatgtaa tagtagtaca
     8521 cactattatt atgtaatagt acttgttgac tgtatttgag aactggaccg agtgagtgtt
     8581 acgtcaccca ttcaaaatga cttacttctg gctccaacaa aatgaagtta attcagttgc
     8641 catttttcac tgtatggaca tcgccgtgtt ggagctagac gttgccaata agcaagaaag
     8701 agccgagatg cgtcgagtct gagtcaccgt tcctatggca acccctctaa ccaatcagaa
     8761 gtaagcttgt tggaagtcca cagcctacca cttgaaagcg ggctgcacaa aatctgtcaa
     8821 acgttttgaa cgttggatgt gagagcacat acttttatta aggcatctgg ttggtcagtt
     8881 tataatatca acaacttggg ctacagaaaa gaaaagttat tacagaaatt atgattaaca
     8941 agtacatgtt aaataaagat tttaatatga atgccaccac tggagcattc atgccatttg
     9001 gagcttcttc ctgtttggat cactagaagg aggaggtcac tcattacagt tctcatatac
     9061 agtcgttggt tggttggttg gttggt
//
