LOCUS       sequence-204093-        9232 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Dox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and
            neo resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9232)
  AUTHORS   Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander 
            EL, Wilkinson JE, Challen GA, Vakoc CR
  TITLE     A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute 
            Myeloid Leukemia.
  JOURNAL   Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 
            10.1016/j.ccell.2017.12.002.
  PUBMED    29316427
REFERENCE   2  (bases 1 to 9232)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2017.12"; journalName: "Cancer Cell"; date: 
            "2018-01-8- 8"; volume: "33"; issue: "1"; pages: "13-28.e8"
COMMENT     Sequence Label: TRIN-shMYB.2572
FEATURES             Location/Qualifiers
     source          1..9232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..86
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        186..565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        566..769
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             770..945
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1008..1207
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1408..1824
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1754..1776
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1792..1808
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     protein_bind    join(1874..1892,1893..1915,1916..1934,1935..1957,1958..1976,
                     1977..1999,2000..2018,2019..2041,2042..2060,2061..2083,
                     2084..2102,2103..2125,2126..2144)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2177..2215
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2212..2236
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2314..2991
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2494..2514)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     2906..2929
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           3060..3181
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3209..3223
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3251..3377
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        3400..3899
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3435..3458)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3832..3851
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(3922..3924,3925..3927,3928..4641)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3967..3988)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4228..4247)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4575..4596
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4673..5246
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4840..4857)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5067..5086
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5262..6056
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5262..6056
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5316..5335)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5926..5945
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6195..6620
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6723..6745)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6881..6901)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6886..7215
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7066..7201
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7237..7256
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7337..7354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7508..8096)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7588..7607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8267..9058,9059..9127))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8890..8909
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9128..9232)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattagctt ggccattgca
      121 tacgttgtat ccatatcata atatgtacat ttatattggc tcatgtccaa cattaccgcc
      181 atgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      241 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      301 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      361 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      421 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      481 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      541 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      601 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      661 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc aataaaagag
      781 cccacaaccc ctcactcggc gcgccagtct tccgatagac tgcgtcgccc gggtacccgt
      841 attcccaata aagcctcttg ctgtttgcat ccgaatcgtg gtctcgctgt tccttgggag
      901 ggtctcctct gagtgattga ctacccacga cgggggtctt tcatttgggg gctcgtccgg
      961 gatttggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca
     1021 acttatctgt gtctgtccga ttgtctagtg tctatgtttg atgttatgcg cctgcgtctg
     1081 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttctga
     1141 acacccggcc gcaaccctgg gagacgtccc agggactttg ggggccgttt ttgtggcccg
     1201 acctgaggaa gggagtcgat gtggaatccg accccgtcag gatatgtggt tctggtagga
     1261 gacgagaacc taaaacagtt cccgcctccg tctgaatttt tgctttcggt ttggaaccga
     1321 agccgcgcgt cttgtctgct gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact
     1381 gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt aagtttgacc
     1441 ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag
     1501 agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga
     1561 gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc
     1621 ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac
     1681 ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc
     1741 gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca
     1801 gccctcactc cttctctagg cgccggaatt gaagatctgg gggatcgatc ctctagagca
     1861 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     1921 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     1981 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2041 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     2101 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc
     2161 tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt
     2221 agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca
     2281 ccgggaccga tccagcctct cgacggatcc accatggcct cctccgagaa cgtcatcacc
     2341 gagttcatgc gcttcaaggt gcgcatggag ggcaccgtga acggccacga gttcgagatc
     2401 gagggcgagg gcgagggccg cccctacgag ggccacaaca ccgtgaagct gaaggtgacc
     2461 aagggcggcc ccctgccctt cgcctgggac atcctgtccc cccagttcca gtacggctcc
     2521 aaggtgtacg tgaagcaccc cgccgacatc cccgactaca agaagctgtc cttccccgag
     2581 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggcgac cgtgacccag
     2641 gactcctccc tgcaggacgg ctgcttcatc tacaaggtga agttcatcgg cgtgaacttc
     2701 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc caccgagcgc
     2761 ctgtaccccc gcgacggcgt gctgaagggc gagacccaca aggccctgaa gctgaaggac
     2821 ggcggccact acctggtgga gttcaagtcc atctacatgg ccaagaagcc cgtgcagctg
     2881 cccggctact actacgtgga cgccaagctg gacatcacct cccacaacga ggactacacc
     2941 atcgtggagc agtacgagcg caccgagggc cgccaccacc tgttcctgta gcggccgcga
     3001 ctctagaatt tactagtcat atgataagat ctctcgacta gggataacag ggtaattgtt
     3061 tgaatgaggc ttcagtactt tacagaatcg ttgcctgcac atcttggaaa cacttgctgg
     3121 gattacttct tcaggttaac ccaacagaag gctcgagaag gtatattgct gttgacagtg
     3181 agcgctccat gtatctcagt cactaatagt gaagccacag atgtattagt gactgagata
     3241 catggaatgc ctactgcctc ggaattcaag gggctacttt aggagcaatt atcttgttta
     3301 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
     3361 ataaattaaa tcactttttt caattctagc aattctaccg ggtaggggag gcgcttttcc
     3421 caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg
     3481 gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg
     3541 gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc
     3601 agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga
     3661 tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc
     3721 tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca
     3781 ggggcgggct caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca
     3841 cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga
     3901 cctgcagccc aagcttccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     3961 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4021 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     4081 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     4141 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4201 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4261 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4321 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4381 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     4441 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4501 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
     4561 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4621 atggacgagc tgtacaagta agaattgttc aattccgccc ctctcccccc cccccctctc
     4681 ccyccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     4741 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     4801 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     4861 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     4921 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     4981 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5041 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5101 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5161 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5221 ttttcctttg aaaaacacga tgataagctt accggtccac catgattgaa caagatggat
     5281 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     5341 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     5401 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc
     5461 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     5521 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     5581 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     5641 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     5701 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     5761 cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga
     5821 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     5881 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     5941 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6001 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgatgta
     6061 cgatgcatcg ataccgtcga gatatcagtg gtccaggctc tagttttgac tcaacaatat
     6121 caccagctga agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc
     6181 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg
     6241 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca
     6301 ggaacagatg gaacagggtc gaccctagag aaccatcaga tgtttccagg gtgccccaag
     6361 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     6421 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc
     6481 agtcctccga ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     6541 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     6601 gtcagcgggg gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac
     6661 caccgaccca ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa
     6721 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6781 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
     6841 acccagtcac gtagcgatag cggagtgtag atccggctgt ggaatgtgtg tcagttaggg
     6901 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     6961 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7021 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7081 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7141 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7201 ctaggctttt gcaaaaagct tactggctta actatgcggc atcagagcag attgtactga
     7261 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7321 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7381 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     7441 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7501 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7561 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7621 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7681 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7741 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7801 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7861 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7921 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7981 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8041 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8101 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8161 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8221 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8281 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8341 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8401 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8461 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8521 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8581 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8641 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8701 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8761 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8821 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8881 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8941 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9001 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9061 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9121 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9181 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cg
//
