LOCUS       sequence-204769-        3882 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Cloning vector including C-terminally GUS-tagged Arabidopsis auxin 
            receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Uchida N, Takahashi K, Iwasaki R, Yamada R, Yoshimura M, Endo TA, 
            Kimura S, Zhang H, Nomoto M, Tada Y, Kinoshita T, Itami K, Hagihara 
            S, Torii KU
  TITLE     Chemical hijacking of auxin signaling with an engineered auxin-TIR1 
            pair.
  JOURNAL   Nat Chem Biol. 2018 Mar;14(3):299-305. doi: 10.1038/nchembio.2555. 
            Epub 2018 Jan 22.
  PUBMED    29355850
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.2555"; journalName: "Nat Chem Biol"; date: 
            "2018-03"; volume: "14"; issue: "3"; pages: "299-305"
COMMENT     Sequence Label: pAN19-ccvTIR1-GUS-NOSt
FEATURES             Location/Qualifiers
     source          1..3882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1807..3618
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3630..3882
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 atgcagaagc gaatagcctt gtcgtttcca gaagaggtac tagagcatgt gttctcgttt
       61 attcagctgg ataaggatag gaactcagtc tctctggtgt gcaagtcatg gtacgagatc
      121 gagcggtggt gcaggaggaa agtcttcatc gggaactgct acgccgtgag tccagcgacg
      181 gtgattagga ggttcccgaa agtgagatcc gtggagctta aaggaaaacc tcacggtgct
      241 gactttaatt tggtacctga cggatgggga ggttacgtgt atccatggat tgaggccatg
      301 tcttcgtctt acacgtggct tgaagagata aggctgaaga ggatggtggt caccgacgat
      361 tgcttggagc tcatagccaa gtcttttaag aattttaagg tactagtgct ttcttcctgc
      421 gaaggcttct ccaccgatgg tctcgctgct atcgctgcca cttgcaggaa tctgaaagag
      481 cttgacttac gagagagtga tgttgacgac gttagtggcc actggcttag ccatttccca
      541 gatacataca cttctttggt atcactcaat atatcttgct tagcatctga ggtcagtttc
      601 tctgctctgg aaaggctggt gactaggtgt cccaatctca agtctctcaa gcttaaccga
      661 gctgttccac ttgaaaaatt ggctacttta cttcaaagag cacctcaatt ggaggaattg
      721 ggcactggtg ggtacactgc agaagtgcga ccagatgttt actctggttt atctgtagcg
      781 ctctctgggt gcaaggaatt gaggtgctta tctggatttt gggatgctgt tcctgcctat
      841 cttccagcag tttattcggt ttgcagtcgg cttacaactt tgaatctgag ttatgcaaca
      901 gtccagagct atgatcttgt caagcttctt tgtcaatgcc ctaaactgca gcgcctctgg
      961 gtgcttgact acatcgagga tgctggtctt gaggtgcttg cttcaacctg caaggaccta
     1021 cgcgagctga gagtgtttcc gtccgagcct tttgtcatgg aaccaaatgt ggcattgacg
     1081 gaacaggggc ttgtctccgt ctccatgggc tgtccaaaac tcgagtcggt tctctacttc
     1141 tgccgtcaaa tgaccaatgc tgcattgata accattgcta ggaaccgtcc caacatgact
     1201 cgcttccgtt tgtgcatcat tgagccaaaa gccccagact atctgactct agagccactg
     1261 gatattggat ttggagccat agtagagcac tgcaaggatc tccgtcgcct ctctctatct
     1321 ggcctcttga ccgacaaggt ttttgaatac attgggacat atgccaagaa gatggaaatg
     1381 ctctcagtgg catttgcagg agacagtgac ttaggcatgc atcatgtttt gtccgggtgc
     1441 gatagcttga ggaaactaga gataagggac tgcccgtttg gagacaaggc gcttttggcc
     1501 aatgcttcaa agctggagac aatgcgatcc ctttggatgt cttcttgttc cgtgagtttt
     1561 ggagcctgca agttactagg acagaagatg ccaaagctga atgtggaagt catcgatgaa
     1621 cggggtgcac cggactcgag accagagagc tgccctgttg agagagtctt catataccga
     1681 acagtggctg gtcctcgatt tgacatgcct ggcttcgtct ggaacatgga ccaagactca
     1741 acaatgaggt tttccaggca aatcattact actaacggat tagtcgaggg tggctcgacc
     1801 ggtggtatgt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact cgacggcctg
     1861 tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg ggaaagcgcg
     1921 ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt cgccgatgca
     1981 gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat accgaaaggt
     2041 tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg
     2101 gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga agccgatgtc
     2161 acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg
     2221 aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa gaaaaagcag
     2281 tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat gctctacacc
     2341 acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca agactgtaac
     2401 cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga actgcgtgat
     2461 gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca agtggtgaat
     2521 ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc
     2581 cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc agtgaagggc
     2641 gaacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg tcatgaagat
     2701 gcggacttgc gtggcaaagg attcgataac gtgctgatgg tgcacgacca cgcattaatg
     2761 gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga agagatgctc
     2821 gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt cggctttaac
     2881 ctctctttag gcattggttt cgaagcgggc aacaagccga aagaactgta cagcgaagag
     2941 gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct gatagcgcgt
     3001 gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga tacccgtccg
     3061 caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg
     3121 cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac catcagcgat
     3181 ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag cggcgatttg
     3241 gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa actgcatcag
     3301 ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc aatgtacacc
     3361 gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg cgtctttgat
     3421 cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc gacctcgcaa
     3481 ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg caaaccgaag
     3541 tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga aaaaccgcag
     3601 cagggaggca aacaatgaga gctctccccg atcgttcaaa catttggcaa taaagtttct
     3661 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     3721 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     3781 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     3841 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tc
//
