LOCUS       sequence-205547-       10052 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-332
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     969..988
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1008..1085)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1291..1310
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1394..1413
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1394..1412
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1413..1437
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1491..1508
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5056..5145
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5158..5247
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6380..6398)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6394..6441
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6478..6933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6565..6584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6775..6796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7025..7840)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7025..7840)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7748..7767
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7832..7851
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7962..8550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8451..8470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8704..8721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8736..8875
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8801..8820)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8961..8983
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8977..9168)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9977..10052,1..1007))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgacacggg caacagctga
       61 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      121 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcctcg
      181 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      241 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      301 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
      361 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      421 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      481 gcgaccagat gctccacgcc cagtcgcgta ccgtcctcat gggagaaaat aatactgttg
      541 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      601 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      661 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      721 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
      781 ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc
      841 agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt
      901 tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa
      961 gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg
     1021 aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg
     1081 gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag
     1141 taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc
     1201 caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag
     1261 cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac
     1321 cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc
     1381 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
     1441 ctagaaataa ttttgcctta actttaagaa ggagatatac catgggcagc caccaccacc
     1501 accaccacag cggaatgcag atctttgtta agactctcac cggaaagaca atcaccctag
     1561 aggtggaaag ctccgacacc atcgacaacg ttaaggccaa gatccaggat aaggagggca
     1621 ttcctccgga tcagcagagg cttattttcg ccggcaagca gctagaggat ggccgtacgt
     1681 tggctgatta caatatccag aaggaatcca ccctccactt ggtcctcagg ctccgtggtg
     1741 gttaagcttg caattaactt taagaaggag atatacaatg cttcacaagc gagctagtga
     1801 agctaacgat aagaacgata ataccatcat cggttccgat ttagcatcat ctaagaaacg
     1861 ccgtatcgat ttcactgaat cttcgtctga taagtcatct tcgattttag cttctggtag
     1921 tagcaggggt tttcacggcg atagcgtcgt tcagcaaatc gacatggctt ttggtaattc
     1981 gaaccgtcag gagattgatg aagatctgca cagtcgccag ctcgccgtct atggtcgtga
     2041 gactatgagg cgtctctttg cttccaatgt tctcatctcg gggatgcacg gtcttggtgc
     2101 cgagattgcc aagaatctta tacttgctgg tgtgaagtct gtgaccttgc atgatgaaag
     2161 agttgtagag ctatgggact tatcaagcaa ctttgttttc tcggaggatg atgttggcaa
     2221 gaatagagca gatgcttctg ttcagaagtt acaggatctt aacaatgctg tggttgtctc
     2281 tagcttgacc aaaagcttaa acaaagagga tctttctggc ttccaggttg ttgtattctc
     2341 tgacataagc atggaaagag caatcgaatt tgatgactat tgtcacagcc atcagcctcc
     2401 tatcgctttt gtcaaggctg atgtcagggg tcttttcggc tctgtctttt gtgattttgg
     2461 gcctgaattt gcggttcttg atgttgatgg agaggaaccc catacaggca tcattgcgtc
     2521 tatctctaat gagaaccagg catttatttc ctgtgtcgac gatgagaggc ttgagtttga
     2581 agatggtgac ctagttgttt tctcagaagt tgagggtatg acagaattga atgatgggaa
     2641 accaaggaag ataaaaagta cacggccata ttcattcacc cttgatgagg acactactaa
     2701 ctatggaaca tatgtgaagg gtggtattgt cactcaagtg aaacagccaa agttactgaa
     2761 tttcaagcca ttgagggaag cgcttaagga tccaggggat tttctattta gtgatttctc
     2821 taagtttgat cggcctccac tccttcactt agcattccag gcacttgatc attttaaagc
     2881 tgaagctggc agatttcctg ttgctggctc agaagaggac gctcagaagc ttatatcgat
     2941 tgccacagcc atcaataccg ggcagggtga tttaaaggtg gagaatgttg atcagaagct
     3001 tctaagacac ttttcctttg gagccaaggc tgtcctgaat cctatggctg caatgtttgg
     3061 cggtattgtt ggacaggagg ttgttaaagc ttgctctgga aaatttcatc ctctcttcca
     3121 gtttttctac tttgattcag tggagtcact cccctctgaa cctgtggact cgagtgactt
     3181 tgcaccaaga aacagccggt atgatgccca aatatctgta tttggggcca agttccagaa
     3241 gaaacttgaa gatgctaaag ttttcacagt agggtctggt gctcttggct gtgagttctt
     3301 gaaaaatttg gctctgatgg gggtttcttg tggaagccaa gggaagctga cagtgacaga
     3361 tgatgatatc attgagaaga gtaacctcag tcgtcaattt ctgttccgtg actggaacat
     3421 tggacaggct aaatccacag ttgctgcttc ggctgctgct gttataaatc ctcgcttcaa
     3481 cattgaggcc ctgcaaaacc gtgttggcgc ggagactgag aatgtatttg acgatgcctt
     3541 ctgggagaac ttaactgttg tcgtcaatgc actggataat gttaatgcga ggctctatgt
     3601 tgattcgagg tgcttgtatt tccagaagcc tctccttgag tctggaactc ttggtacaaa
     3661 gtgcaacaca cagtcggtta tcccacatct aactgaaaat tacggtgcct caagggaccc
     3721 accagagaaa caggccccca tgtgtacagt gcattccttc ccgcataaca ttgatcactg
     3781 tttaacttgg gctcgctctg agtttgaggg tctgctggag aaaactcccg ctgaagtgaa
     3841 tgcatatctt tctagcccag ttgagtacac taactcaatg atgagtgctg gtgatgctca
     3901 ggcgagggac acattggaga ggattgttga gtgccttgaa aaggagaagt gtgaaacctt
     3961 ccaagactgc ttaacctggg cacgactgag gtttgaggat tactttgtga accgtgtcaa
     4021 gcaattgata tacacatttc ctgaagatgc tgcgactagt accggagctc cattctggtc
     4081 tgctccaaaa agattccctc gtccactcca gtactcgtcg tccgacccaa gtctccttaa
     4141 ctttatcaca gcgactgcta ttttacgagc agagacattt gggatcccga tacctgagtg
     4201 gactaagaac ccaaaggaag cagcagaagc tgttgacaga gtgatagtcc cagactttga
     4261 gccaaggcaa gatgcaaaga ttgtgactga tgagaaagct accactttaa ccactgcttc
     4321 ggtggacgac gctgcagtca tcgacgatct cattgctaag atcgaccagt gtaggcataa
     4381 cttgtcgcca gacttcagga tgaaacctat tcagttcgaa aaggatgatg atacaaacta
     4441 tcacatggat gtgatagcag gactagccaa catgagggcg agaaattaca gcatccctga
     4501 agtggacaag ctgaaagcaa agttcatagc agggagaatc atcccagcca ttgcgacttc
     4561 aacagccatg gctactgggc tggtctgcct cgagctgtac aaggtccttg atggaggaca
     4621 taaagtggaa gcttacagaa acacatttgc caacctggca cttccactat tctccatggc
     4681 tgaaccactt ccaccaaagg tggtgaagca ccgtgacatg gcttggacag tttgggacag
     4741 atgggttctc aagggaaacc caactcttcg tgaggtgttg cagtggctgg aggacaaagg
     4801 gcttagcgca tacagcatct cttgtggaag ctgtctactg ttcaacagta tgttcacaag
     4861 gcacaaggag aggatggaca agaaggttgt ggatctcgct cgggatgttg cgaaagtcga
     4921 gttaccgcct tatcgcaacc atcttgatgt agtggtggct tgtgaggatg aagatgacaa
     4981 tgacgtcgat attcctctcg tctctatcta cttcaggtga gcttactatt aactttaaga
     5041 aggagatata ccatgtaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc
     5101 ccggactatg caggattgta tccatatgac gttccagatt acgccactag agctgcttac
     5161 ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggattg
     5221 tatccatatg acgttccaga ttacgcaatg gccaacagta atttgccgcg aagaatcatc
     5281 aaggaaactc aacgtctgct cagtgaaccg gctcccggta taagtgcctc tccatctgag
     5341 gataatatgc ggtatttcaa cgttatgatt cttggtccta cacaatcacc ttatgaagga
     5401 ggagttttca agttggagct ctttttgcct gaagaatacc ctatggcagc tcccaaggtt
     5461 aggtttctca caaagatata ccatcctaac attgacaagc ttggaagaat ctgtcttgat
     5521 attctcaagg acaaatggag ccctgcacta caaatacgaa cagtgctctt aagtattcaa
     5581 gctcttctga gtgcaccaaa cccagatgat ccgctttctg agaacattgc taagcactgg
     5641 aagagtaatg aggcagaagc tgtggataca gctaaggaat ggacccgtct ttatgcaagc
     5701 ggcgcatgag cttttactta actttaagaa ggagatatac aatgggttcg gaagaagaga
     5761 aggtcgttgt tcccagaaac tttagattat tggaagagct agaaagaggt gagaaaggaa
     5821 ttggagatgg gacagtgagt tatggaatgg atgatgctga tgatattttg atgcaatctt
     5881 ggactggtac cattcttggt cctcataata ctgcgtatga agggaaaatc tttcagctga
     5941 agctattctg tggaaaggat tatccggaaa gtccacctac tgtgaggttt cagagtcgta
     6001 taaacatggc ttgtgtcaat cctgagaatg gagtggttga tccgagtcac ttccctatgc
     6061 tttccaactg gagaagagaa ttcacaatgg aggatctact gattcagctt aaaaaagaaa
     6121 tgatgtcatc gcagaaccgc aagttagctc aacccttgga aggtaatgag gaaggcagga
     6181 cagacccaaa gggactagtg gtgaaatgtt gcgtgatgtg agcttcagag cttttaagcc
     6241 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     6301 ccgcttacag acaagctgtg accgggatga gatccggctg ctaacaaagc ccgaaaggaa
     6361 gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa
     6421 cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg
     6481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6901 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
     6961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta
     7021 attcttagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     7081 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     7141 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     7201 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     7261 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     7321 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     7381 attgcgcctg agcgagtcga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     7441 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     7501 gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg
     7561 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     7621 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     7681 gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc
     7741 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     7801 gcggcctaga gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt
     7861 ttatgtaagc agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc
     7921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8101 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
     8761 catctgtgcg gtatttcaca ccgcaatggt gcactctcag tacaatctgc tctgatgccg
     8821 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     8881 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     8941 cagacaagct gtgaccgact ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     9001 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
     9061 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
     9121 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
     9181 gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
     9241 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     9301 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     9361 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
     9421 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaacccaa
     9481 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     9541 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     9601 cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc
     9661 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     9721 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     9781 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     9841 cacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     9901 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
     9961 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10021 ttaatgaatc ggccaacgcg cggggagagg cg
//
