LOCUS       sequence-205899-        2387 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid donor for expression of proteins under the control of a 
            minimal HSP70 promoter in baculovirus/insect cell systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Monika Golas
  TITLE     Plasmids for expression of proteins in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGS-BacD-23003
FEATURES             Location/Qualifiers
     source          1..2387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     197..218
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(322..341)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    366..414
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    616..649
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      651..1007
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1368..2162
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1422..1441)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2032..2051
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gatactagtc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
       61 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
      121 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
      181 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagaa
      241 gacttgatca cccgggatct cgagccatgg tgctagcagc tgatgcatag catgcggtac
      301 cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc
      361 tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt gttcataaac
      421 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattgggacc
      481 aatacgcccg cgtttcttcc ttttccccac cccaaccccc aagttcgggt gaaggcccag
      541 ggctcgcagc caacgtcggg gcggcaagcc ctgccatagc cactacgggt acgtttaaac
      601 ccatgtgcct ggcagataac ttcgtataat gtatgctata cgaagttatg gtacgtacta
      661 agctctcatg tttcacgtac taagctctca tgtttaacgt actaagctct catgtttaac
      721 gaactaaacc ctcatggcta acgtactaag ctctcatggc taacgtacta agctctcatg
      781 tttcacgtac taagctctca tgtttgaaca ataaaattaa tataaatcag caacttaaat
      841 agcctctaag gttttaagtt ttataagaaa aaaaagaata tataaggctt ttaaagcttt
      901 taaggtttaa cggttgtgga caacaagcca gggatgtaac gcactgagaa gcccttagag
      961 cctctcaaag caattttcag tgacacagga acacttaacg gctgacagaa ttagcttcac
     1021 gctgccgcaa gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca
     1081 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     1141 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     1201 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     1261 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
     1321 gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     1381 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     1441 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     1501 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     1561 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     1621 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     1681 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     1741 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     1801 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     1861 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     1921 tcgtgacaca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     1981 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     2041 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     2101 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     2161 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     2221 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     2281 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccgggat
     2341 ctatgtcggg tgcggagaaa gaggtaatga aatggcacct aggtatc
//
