LOCUS       sequence-205900-        2337 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid donor for expression of proteins under the control of a 
            minimal HSP70 promoter in baculovirus/insect cell systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Monika Golas
  TITLE     Plasmids for expression of proteins in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGS-BacD-23004
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     198..219
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(323..342)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    367..415
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    617..650
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      652..1008
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1471..2262
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt cgccggagta taaatagagg cgcttcgtct acggagcgac aattcaattc
       61 aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa gcgcagctga
      121 acaagctaaa caatctgcag taaagtgcaa gttaaagtga atcaattaaa agtaaccagc
      181 aaccaagtaa atcaactgca actactgaaa tctgccaaga agtaattatt gaatacaaga
      241 agacttgatc acccgggatc tcgagccatg gtgctagcag ctgatgcata gcatgcggta
      301 ccgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
      361 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
      421 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattgggac
      481 caatacgccc gcgtttcttc cttttcccca ccccaacccc caagttcggg tgaaggccca
      541 gggctcgcag ccaacgtcgg ggcggcaagc cctgccatag ccactacggg tacgtttaaa
      601 cccatgtgcc tggcagataa cttcgtataa tgtatgctat acgaagttat ggtacgtact
      661 aagctctcat gtttcacgta ctaagctctc atgtttaacg tactaagctc tcatgtttaa
      721 cgaactaaac cctcatggct aacgtactaa gctctcatgg ctaacgtact aagctctcat
      781 gtttcacgta ctaagctctc atgtttgaac aataaaatta atataaatca gcaacttaaa
      841 tagcctctaa ggttttaagt tttataagaa aaaaaagaat atataaggct tttaaagctt
      901 ttaaggttta acggttgtgg acaacaagcc agggatgtaa cgcactgaga agcccttaga
      961 gcctctcaaa gcaattttga gtgacacagg aacacttaac ggctgacata attcagcttc
     1021 acgctgccgc aagcactcag ggcgcaaggg ctgctaaagg aagcggaaca cgtagaaagc
     1081 cagtccgcag aaacggtgct gaccccggat gaatgtcagc tgggaggcag aataaatgat
     1141 catatcgtca attattacct ccacggggag agcctgagca aactggcctc aggcatttga
     1201 gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaagtagcg tatgcgctca
     1261 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1321 atggcttgtt atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa
     1381 gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag
     1441 ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc cgaagtatcg
     1501 actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc
     1561 gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg
     1621 ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt
     1681 ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt
     1741 gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga
     1801 gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat
     1861 ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg
     1921 gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta
     1981 acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg
     2041 tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac
     2101 tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct
     2161 tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc
     2221 cactacgtga aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca
     2281 agccgacgga tctatgtcgg gtgcggagaa agaggtaatg aaatggcacc taggtat
//
