LOCUS       sequence-205902-        2368 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid donor for expression of proteins under the control of a 
            minimal HSP70 promoter in baculovirus/insect cell systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Monika Golas
  TITLE     Plasmids for expression of proteins in insect cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGS-BacD-23005
FEATURES             Location/Qualifiers
     source          1..2368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..41)
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     protein_bind    244..277
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      330..686
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1078..1737)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1652..1671
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1738..1840)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     polyA_signal    2012..2146
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2036..2055)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2090..2109
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttgtattcaa taattacttc ttggcagatt tcagtagttg cagttgattt acttggttgc
       61 tggttacttt taattgattc actttaactt gcactttact gcagattgtt tagcttgttc
      121 agctgcgctt gtttatttgc ttagctttcg cttagcgacg tgttcacttt gcttgtttga
      181 attgaattgt cgctccgtag acgaagcgcc tctatttata ctccggcgcc atgtgcctgg
      241 cagataactt cgtataatgt atgctatacg aagttatggt accgcggccg cgtagaggat
      301 ctgttgatca gcagttcaac ctgttgatag tacgtactaa gctctcatgt ttcacgtact
      361 aagctctcat gtttaacgta ctaagctctc atgtttaacg aactaaaccc tcatggctaa
      421 cgtactaagc tctcatggct aacgtactaa gctctcatgt ttcacgtact aagctctcat
      481 gtttgaacaa taaaattaat ataaatcagc aacttaaata gcctctaagg ttttaagttt
      541 tataagaaaa aaaagaatat ataaggcttt taaagctttt aaggtttaac ggttgtggac
      601 aacaagccag ggatgtaacg cactgagaag cccttagagc ctctcaaagc aattttgagt
      661 gacacaggaa cacttaacgg ctgacagaat tagcttcacg ctgccgcaag cactcagggc
      721 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
      781 cccggatgaa tgtcagctgg gaggcagaat aaatgatcat atcgtcaatt attacctcca
      841 cggggagagc ctgagcaaac tggcctcagg catttgagaa gcacacggtc acactgcttc
      901 cggtagtcaa taaaccggta aaccagcaat agacataagc ggctatttaa cgaccctgcc
      961 ctgaaccgac gaccgggtcg aatttgcttt cgaatttctg ccattcatcc gcttattatc
     1021 acttattcag gcgtagcaac caggcgttta agggcaccaa taactgcctt aaaaaaatta
     1081 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
     1141 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
     1201 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
     1261 gtttaaatca aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc
     1321 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
     1381 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
     1441 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
     1501 gtctttcatt gccatacgga attccggatg agcattcatc aggcgggcaa gaatgtgaat
     1561 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
     1621 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
     1681 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
     1741 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
     1801 ttcattatgg tgaaagttgg accctcttac gtgccgatca acgtctcatt ttcgccaaaa
     1861 gttggcccag atctatgtcg ggtgcggaga aagaggtaat gaaatggcac ctaggggtta
     1921 tgatagttat tgctcagcgg tggcagcagc caactcagct tcctttcggg ctttgttagc
     1981 agccggatct tctaggctca agcagtgatc agatccagac atgataagat acattgatga
     2041 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2101 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2161 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2221 cctctacaaa tgtggtatgg ctgattatga tcctctagta cttctcgaca agcttgtcga
     2281 gactgcaggc tctagattcg aaagcggccg cgactagtga gctcgtcgac gtaggccttt
     2341 gaattccgcg cgcttcggac cgggatcc
//
