LOCUS       sequence-206008-        5144 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  This construct can be used in the BioID process to identify proteins
            that interact with the Insulin-like Growth Factor 1 Receptor 
            (IGF1R)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Bareja A, Hodgkinson CP, Soderblom E, Waitt G, Dzau VJ
  TITLE     The proximity-labeling technique BioID identifies sorting nexin 6 as
            a member of the insulin-like growth factor 1 (IGF1)-IGF1 receptor 
            pathway.
  JOURNAL   J Biol Chem. 2018 Mar 12. pii: RA118.002406. doi: 
            10.1074/jbc.RA118.002406.
  PUBMED    29530981
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Mar 12. pii: RA118.002406. doi: 
            10.1074/jbc.RA118.002406."
COMMENT     Sequence Label: pcDNA3.1 IGF1R-BirA(R118G)-HA
FEATURES             Location/Qualifiers
     source          1..5144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4152..4499,4500..4502,4503..5111)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRKL
                     GADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQGW
                     ITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGD
                     KEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             5115..5141
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 taatacgact cactataggg agacccaagc tggctagcat gaagtctggc tccggaggag
       61 ggtccccgac ctcgctgtgg gggctcctgt ttctctccgc cgcgctctcg ctctggccga
      121 cgagtggaga aatctgcggg ccaggcatcg acatccgcaa cgactatcag cagctgaagc
      181 gcctggagaa ctgcacggtg atcgagggct acctccacat cctgctcatc tccaaggccg
      241 aggactaccg cagctaccgc ttccccaagc tcacggtcat taccgagtac ttgctgctgt
      301 tccgagtggc tggcctcgag agcctcggag acctcttccc caacctcacg gtcatccgcg
      361 gctggaaact cttctacaac tacgccctgg tcatcttcga gatgaccaat ctcaaggata
      421 ttgggcttta caacctgagg aacattactc ggggggccat caggattgag aaaaatgctg
      481 acctctgtta cctctccact gtggactggt ccctgatcct ggatgcggtg tccaataact
      541 acattgtggg gaataagccc ccaaaggaat gtggggacct gtgtccaggg accatggagg
      601 agaagccgat gtgtgagaag accaccatca acaatgagta caactaccgc tgctggacca
      661 caaaccgctg ccagaaaatg tgcccaagca cgtgtgggaa gcgggcgtgc accgagaaca
      721 atgagtgctg ccaccccgag tgcctgggca gctgcagcgc gcctgacaac gacacggcct
      781 gtgtagcttg ccgccactac tactatgccg gtgtctgtgt gcctgcctgc ccgcccaaca
      841 cctacaggtt tgagggctgg cgctgtgtgg accgtgactt ctgcgccaac atcctcagcg
      901 ccgagagcag cgactccgag gggtttgtga tccacgacgg cgagtgcatg caggagtgcc
      961 cctcgggctt catccgcaac ggcagccaga gcatgtactg catcccttgt gaaggtcctt
     1021 gcccgaaggt ctgtgaggaa gaaaagaaaa caaagaccat tgattctgtt acttctgctc
     1081 agatgctcca aggatgcacc atcttcaagg gcaatttgct cattaacatc cgacggggga
     1141 ataacattgc ttcagagctg gagaacttca tggggctcat cgaggtggtg acgggctacg
     1201 tgaagatccg ccattctcat gccttggtct ccttgtcctt cctaaaaaac cttcgcctca
     1261 tcctaggaga ggagcagcta gaagggaatt actccttcta cgtcctcgac aaccagaact
     1321 tgcagcaact gtgggactgg gaccaccgca acctgaccat caaagcaggg aaaatgtact
     1381 ttgctttcaa tcccaaatta tgtgtttccg aaatttaccg catggaggaa gtgacgggga
     1441 ctaaagggcg ccaaagcaaa ggggacataa acaccaggaa caacggggag agagcctcct
     1501 gtgaaagtga cgtcctgcat ttcacctcca ccaccacgtc gaagaatcgc atcatcataa
     1561 cctggcaccg gtaccggccc cctgactaca gggatctcat cagcttcacc gtttactaca
     1621 aggaagcacc ctttaagaat gtcacagagt atgatgggca ggatgcctgc ggctccaaca
     1681 gctggaacat ggtggacgtg gacctcccgc ccaacaagga cgtggagccc ggcatcttac
     1741 tacatgggct gaagccctgg actcagtacg ccgtttacgt caaggctgtg accctcacca
     1801 tggtggagaa cgaccatatc cgtggggcca agagtgagat cttgtacatt cgcaccaatg
     1861 cttcagttcc ttccattccc ttggacgttc tttcagcatc gaactcctct tctcagttaa
     1921 tcgtgaagtg gaaccctccc tctctgccca acggcaacct gagttactac attgtgcgct
     1981 ggcagcggca gcctcaggac ggctaccttt accggcacaa ttactgctcc aaagacaaaa
     2041 tccccatcag gaagtatgcc gacggcacca tcgacattga ggaggtcaca gagaacccca
     2101 agactgaggt gtgtggtggg gagaaagggc cttgctgcgc ctgccccaaa actgaagccg
     2161 agaagcaggc cgagaaggag gaggctgaat accgcaaagt ctttgagaat ttcctgcaca
     2221 actccatctt cgtgcccaga cctgaaagga agcggagaga tgtcatgcaa gtggccaaca
     2281 ccaccatgtc cagccgaagc aggaacacca cggccgcaga cacctacaac atcaccgacc
     2341 cggaagagct ggagacagag taccctttct ttgagagcag agtggataac aaggagagaa
     2401 ctgtcatttc taaccttcgg cctttcacat tgtaccgcat cgatatccac agctgcaacc
     2461 acgaggctga gaagctgggc tgcagcgcct ccaacttcgt ctttgcaagg actatgcccg
     2521 cagaaggagc agatgacatt cctgggccag tgacctggga gccaaggcct gaaaactcca
     2581 tctttttaaa gtggccggaa cctgagaatc ccaatggatt gattctaatg tatgaaataa
     2641 aatacggatc acaagttgag gatcagcgag aatgtgtgtc cagacaggaa tacaggaagt
     2701 atggaggggc caagctaaac cggctaaacc cggggaacta cacagcccgg attcaggcca
     2761 catctctctc tgggaatggg tcgtggacag atcctgtgtt cttctatgtc caggccaaaa
     2821 caggatatga aaacttcatc catctgatca tcgctctgcc cgtcgctgtc ctgttgatcg
     2881 tgggagggtt ggtgattatg ctgtacgtct tccatagaaa gagaaataac agcaggctgg
     2941 ggaatggagt gctgtatgcc tctgtgaacc cggagtactt cagcgctgct gatgtgtacg
     3001 ttcctgatga gtgggaggtg gctcgggaga agatcaccat gagccgggaa cttgggcagg
     3061 ggtcgtttgg gatggtctat gaaggagttg ccaagggtgt ggtgaaagat gaacctgaaa
     3121 ccagagtggc cattaaaaca gtgaacgagg ccgcaagcat gcgtgaaagg attgagtttc
     3181 tcaacgaagc ttctgtgatg aaggagttca attgtcacca tgtggtgcga ttgctgggtg
     3241 tggtgtccca aggccagcca acactggtca tcatggaact gatgacacgg ggcgatctca
     3301 aaagttatct ccggtctctg aggccagaaa tggagaataa tccagtccta gcacctccaa
     3361 gcctgagcaa gatgattcag atggccggag agattgcaga cggcatggca tacctcaacg
     3421 ccaataagtt cgtccacaga gaccttgctg cccggaattg catggtagcc gaagatttca
     3481 cagtcaaaat cggagatttt ggtatgacgc gagatatcta tgagacagac tattaccgga
     3541 aaggagggaa agggctgctg cccgtgcgct ggatgtctcc tgagtccctc aaggatggag
     3601 tcttcaccac ttactcggac gtctggtcct tcggggtcgt cctctgggag atcgccacac
     3661 tggccgagca gccctaccag ggcttgtcca acgagcaagt ccttcgcttc gtcatggagg
     3721 gcggccttct ggacaagcca gacaactgtc ctgacatgct gtttgaactg atgcgcatgt
     3781 gctggcagta taaccccaag atgaggcctt ccttcctgga gatcatcagc agcatcaaag
     3841 aggagatgga gcctggcttc cgggaggtct ccttctacta cagcgaggag aacaagctgc
     3901 ccgagccgga ggagctggac ctggagccag agaacatgga gagcgtcccc ctggacccct
     3961 cggcctcctc gtcctccctg ccactgcccg acagacactc aggacacaag gccgagaacg
     4021 gccccggccc tggggtgctg gtcctccgcg ccagcttcga cgagagacag ccttacgccc
     4081 acatgaacgg gggccgcaag aacgagcggg ccttgccgct gccccagtct tcgacctgcg
     4141 aattcggatc caaggacaac accgtgcccc tgaagctgat cgccctgctg gccaacggcg
     4201 agttccactc tggcgagcag ctgggagaga ccctgggaat gagcagagcc gccatcaaca
     4261 agcacatcca gacactgaga gactggggag tggacgtgtt caccgtgcct ggcaagggct
     4321 acagcctgcc tgagcctatc cagctgctga acgccaagca gatcctggga cagctggatg
     4381 gcggaagcgt ggccgtgctg cctgtgatcg actccaccaa tcagtacctg ctggacagaa
     4441 tcggagagct gaagtccggc gacgcctgca tcgccgagta ccagcaggct ggcagaggag
     4501 gcagaggacg gaagtggttc agcccattcg gagccaacct gtacctgtcc atgttctgga
     4561 gactggagca gggacctgct gctgccatcg gactgagtct ggtgatcgga atcgtgatgg
     4621 ccgaggtgct gagaaagctg ggagccgaca aggtgagagt gaagtggcct aatgacctgt
     4681 acctccagga ccgcaagctg gctggcatcc tggtggagct gacaggcaag acaggcgatg
     4741 ccgctcagat cgtgatcgga gccggaatca acatggccat gagaagagtg gaggagagcg
     4801 tggtgaacca gggctggatc accctgcagg aggctggcat caacctggac cggaacaccc
     4861 tggccgccat gctgatcaga gagctgagag ccgctctgga gctgttcgag caggagggac
     4921 tggctcctta cctgagcaga tgggagaagc tggacaactt catcaacaga cctgtgaagc
     4981 tgatcatcgg cgacaaggaa atcttcggca tctccagagg aatcgacaag cagggagctc
     5041 tgctgctgga gcaggacgga atcatcaagc cctggatggg cggagaaatc tccctgagaa
     5101 gcgcagagaa ggcttaccct tacgatgtac cggattacgc atag
//
