LOCUS       sequence-206354-        3031 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pJOG684
FEATURES             Location/Qualifiers
     source          1..3031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     188..207
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     441..458
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1356..1374)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1442..1546
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1768..2559
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(2730..3031,1..287)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
       61 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
      121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
      181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
      241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
      301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
      361 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      421 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      481 gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaggagtg
      541 ggacccacgg ttcaattatt gccaattttc agctccaccg tatatttaaa aaataaaacg
      601 ataatgctaa aaaaatataa atcgtaacga tcgttaaatc tcaacggctg gatcttatga
      661 cgaccgttag aaattgtggt tgtcgacgag tcagtaataa acggcgtcaa agtggttgca
      721 gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa
      781 aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt
      841 ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt
      901 tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca attcagattt
      961 caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt
     1021 tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta
     1081 tatgttcttt ggtttagatt ctgttaatct tagatcgaag tcgattttct gggtttgatc
     1141 gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat
     1201 aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt
     1261 agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga ttaacaaatg
     1321 tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc tatttttata
     1381 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     1441 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1501 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa
     1561 ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc
     1621 ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     1681 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     1741 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
     1801 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     1861 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat tgatttgctg
     1921 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     1981 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     2041 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     2101 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     2161 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     2221 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     2281 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     2341 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     2401 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     2461 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccat
     2521 tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca
     2581 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     2641 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     2881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3001 ttggactcaa gacgatagtt accggataag g
//
