LOCUS       sequence-206675-        3304 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Multisite gateway vector for 3' tagging with constitutively active 
            rab5c.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab5c-CA
FEATURES             Location/Qualifiers
     source          1..3304
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(624..748)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1413..1508)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1545..1564)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1546..1564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1569..1585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1569..1585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1687..1706)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1698..2507
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1765..1784)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2654..3242
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3143..3162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggccctg cagctctaga gctcgaattc tacaggtcac
      601 taataccatc taagtagttg gttcatagtg actgcatatg ttgtgtttta cagtattatg
      661 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      721 ctcgttcaac tttcttgtac aaagtggtgg cggggcgagg tggaccagcg cggaccaacg
      781 gtgccgcccc cgtcgggaac aaaatctgcc agtttaagct tgtgttgctg ggagagtctg
      841 cagtaggcaa gtccagcctg gtgctgcgct tcgtcaaagg ccagtttcac gagtaccagg
      901 agagcaccat tggagctgct ttcctcacac agacactctg cttggacgat acgacagtaa
      961 agtttgagat ttgggacacg gccggactgg agcggtatca cagtttggcc cctatgtatt
     1021 acagaggagc ccaggcagcc atcgtcgtct acgacattac taacacagac acattcacta
     1081 gagcaaagaa ctgggtgaag gagctgcagc gccaggccag tccaaatatc gttattgctc
     1141 tggcaggcaa caaagcagat ctggccaaca agagagccgt tgactttcag gaggcccagg
     1201 catacgcaga tgacaacagc ctactcttca tggagacgtc tgcaaagacc gccatgaacg
     1261 tcaatgagat cttcatggct atcgccaaga agcttccgaa aagcgagccg cagggtggag
     1321 cgggcagcgg gggacgggcc cgaggagggg tggacttaca ggagacggcg cctcagggaa
     1381 gaagcggcca gtgctgtgga ggcggaaact aacaacttta ttatacaaag ttggcattat
     1441 aaaaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     1501 cattatttgg agctccatgg tagcgttaac gcggccgcga tatcccctat agtgagtcgt
     1561 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1621 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
     1681 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg cgattaaatt
     1741 ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag
     1801 gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg
     1861 gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac tggctgacgg
     1921 aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac
     1981 tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat cctgattcag
     2041 gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt
     2101 gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga
     2161 ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg cctgttgaac
     2221 aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc gtcactcatg
     2281 gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg
     2341 ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg
     2401 gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg
     2461 atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca gaattggtta
     2521 attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca agctcatgac
     2581 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga
     2641 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2701 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2761 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2821 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2881 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2941 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     3001 tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca
     3061 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     3121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     3181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3301 tgtt
//
