LOCUS       sequence-207214-        9230 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses human codon optimized LbCpf1(RVR mutant) in mammalian 
            cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9230)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3320
FEATURES             Location/Qualifiers
     source          1..9230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             929..4612
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMRGWDKDVETDRRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             4613..4660
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4667..4747
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4821..4838)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4827..5051
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5097..5525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5184..5203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5394..5415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5534..5554)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5539..5868
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5719..5854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5781..5800
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5935..6729
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5989..6008)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6599..6618
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6903..7024
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6940..6959)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6994..7013
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7073..7089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7073..7089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7086..7108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7097..7113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7121..7127,7128..7145,7146..7151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7166..7187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7304..7321)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7475..8063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7555..7574)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8234..9025,9026..9094))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8857..8876
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9095..9199)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgccaccat gagcaagctg gagaagttta caaactgcta
      961 ctccctgtct aagaccctga ggttcaaggc catccctgtg ggcaagaccc aggagaacat
     1021 cgacaataag cggctgctgg tggaggacga gaagagagcc gaggattata agggcgtgaa
     1081 gaagctgctg gatcgctact atctgtcttt tatcaacgac gtgctgcaca gcatcaagct
     1141 gaagaatctg aacaattaca tcagcctgtt ccggaagaaa accagaaccg agaaggagaa
     1201 taaggagctg gagaacctgg agatcaatct gcggaaggag atcgccaagg ccttcaaggg
     1261 caacgagggc tacaagtccc tgtttaagaa ggatatcatc gagacaatcc tgccagagtt
     1321 cctggacgat aaggacgaga tcgccctggt gaacagcttc aatggcttta ccacagcctt
     1381 caccggcttc tttgataaca gagagaatat gttttccgag gaggccaaga gcacatccat
     1441 cgccttcagg tgtatcaacg agaatctgac ccgctacatc tctaatatgg acatcttcga
     1501 gaaggtggac gccatctttg ataagcacga ggtgcaggag atcaaggaga agatcctgaa
     1561 cagcgactat gatgtggagg atttctttga gggcgagttc tttaactttg tgctgacaca
     1621 ggagggcatc gacgtgtata acgccatcat cggcggcttc gtgaccgaga gcggcgagaa
     1681 gatcaagggc ctgaacgagt acatcaacct gtataatcag aaaaccaagc agaagctgcc
     1741 taagtttaag ccactgtata agcaggtgct gagcgatcgg gagtctctga gcttctacgg
     1801 cgagggctat acatccgatg aggaggtgct ggaggtgttt agaaacaccc tgaacaagaa
     1861 cagcgagatc ttcagctcca tcaagaagct ggagaagctg ttcaagaatt ttgacgagta
     1921 ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc agcacaatct ccaaggatat
     1981 cttcggcgag tggaacgtga tccgggacaa gtggaatgcc gagtatgacg atatccacct
     2041 gaagaagaag gccgtggtga ccgagaagta cgaggacgat cggagaaagt ccttcaagaa
     2101 gatcggctcc ttttctctgg agcagctgca ggagtacgcc gacgccgatc tgtctgtggt
     2161 ggagaagctg aaggagatca tcatccagaa ggtggatgag atctacaagg tgtatggctc
     2221 ctctgagaag ctgttcgacg ccgattttgt gctggagaag agcctgaaga agaacgacgc
     2281 cgtggtggcc atcatgaagg acctgctgga ttctgtgaag agcttcgaga attacatcaa
     2341 ggccttcttt ggcgagggca aggagacaaa cagggacgag tccttctatg gcgattttgt
     2401 gctggcctac gacatcctgc tgaaggtgga ccacatctac gatgccatcc gcaattatgt
     2461 gacccagaag ccctactcta aggataagtt caagctgtat tttcagaacc ctcagttcat
     2521 gaggggctgg gacaaggatg tggagaccga tcggcgggcc accatcctga gatacggctc
     2581 caagtactat ctggccatca tggataagaa gtacgccaag tgcctgcaga agatcgacaa
     2641 ggacgatgtg aacggcaatt acgagaagat caactataag ctgctgcccg gccctaataa
     2701 gatgctgcca aaggtgttct tttctaagaa gtggatggcc tactataacc ccagcgagga
     2761 catccagaag atctacaaga atggcacatt caagaagggc gatatgttta acctgaatga
     2821 ctgtcacaag ctgatcgact tctttaagga tagcatctcc cggtatccaa agtggtccaa
     2881 tgcctacgat ttcaactttt ctgagacaga gaagtataag gacatcgccg gcttttacag
     2941 agaggtggag gagcagggct ataaggtgag cttcgagtct gccagcaaga aggaggtgga
     3001 taagctggtg gaggagggca agctgtatat gttccagatc tataacaagg acttttccga
     3061 taagtctcac ggcacaccca atctgcacac catgtacttc aagctgctgt ttgacgagaa
     3121 caatcacgga cagatcaggc tgagcggagg agcagagctg ttcatgaggc gcgcctccct
     3181 gaagaaggag gagctggtgg tgcacccagc caactcccct atcgccaaca agaatccaga
     3241 taatcccaag aaaaccacaa ccctgtccta cgacgtgtat aaggataaga ggttttctga
     3301 ggaccagtac gagctgcaca tcccaatcgc catcaataag tgccccaaga acatcttcaa
     3361 gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat aacccctatg tgatcggcat
     3421 cgataggggc gagcgcaatc tgctgtatat cgtggtggtg gacggcaagg gcaacatcgt
     3481 ggagcagtat tccctgaacg agatcatcaa caacttcaac ggcatcagga tcaagacaga
     3541 ttaccactct ctgctggaca agaaggagaa ggagaggttc gaggcccgcc agaactggac
     3601 ctccatcgag aatatcaagg agctgaaggc cggctatatc tctcaggtgg tgcacaagat
     3661 ctgcgagctg gtggagaagt acgatgccgt gatcgccctg gaggacctga actctggctt
     3721 taagaatagc cgcgtgaagg tggagaagca ggtgtatcag aagttcgaga agatgctgat
     3781 cgataagctg aactacatgg tggacaagaa gtctaatcct tgtgcaacag gcggcgccct
     3841 gaagggctat cagatcacca ataagttcga gagctttaag tccatgtcta cccagaacgg
     3901 cttcatcttt tacatccctg cctggctgac atccaagatc gatccatcta ccggctttgt
     3961 gaacctgctg aaaaccaagt ataccagcat cgccgattcc aagaagttca tcagctcctt
     4021 tgacaggatc atgtacgtgc ccgaggagga tctgttcgag tttgccctgg actataagaa
     4081 cttctctcgc acagacgccg attacatcaa gaagtggaag ctgtactcct acggcaaccg
     4141 gatcagaatc ttccggaatc ctaagaagaa caacgtgttc gactgggagg aggtgtgcct
     4201 gaccagcgcc tataaggagc tgttcaacaa gtacggcatc aattatcagc agggcgatat
     4261 cagagccctg ctgtgcgagc agtccgacaa ggccttctac tctagcttta tggccctgat
     4321 gagcctgatg ctgcagatgc ggaacagcat cacaggccgc accgacgtgg attttctgat
     4381 cagccctgtg aagaactccg acggcatctt ctacgatagc cggaactatg aggcccagga
     4441 gaatgccatc ctgccaaaga acgccgacgc caatggcgcc tataacatcg ccagaaaggt
     4501 gctgtgggcc atcggccagt tcaagaaggc cgaggacgag aagctggata aggtgaagat
     4561 cgccatctct aacaaggagt ggctggagta cgcccagacc agcgtgaagc acaaaaggcc
     4621 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     4681 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     4741 ctatgcctaa gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc
     4801 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     4861 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     4921 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     4981 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     5041 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     5101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     5161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     5221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     5281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     5341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     5401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     5461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     5521 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     5581 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     5641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     5701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     5821 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     5881 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     5941 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6001 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     6061 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     6121 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     6181 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     6241 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     6301 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     6361 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     6421 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     6481 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     6541 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     6601 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     6661 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     6721 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     6781 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     6841 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     6901 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     6961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7021 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7081 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7321 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7381 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7441 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7501 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7561 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7621 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7681 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7741 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7801 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7861 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7921 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7981 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8041 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8101 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8161 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8221 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8281 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8341 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8401 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8461 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8521 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8581 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8641 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8701 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8761 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8821 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8881 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8941 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9001 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9061 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9121 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9181 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
