LOCUS       sequence-207236-        9722 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Toth E, Czene BC, Kulcsar PI, Krausz SL, Talas A, Nyeste A, Varga E,
            Huszar K, Weinhardt N, Ligeti Z, Borsy AE, Fodor E, Welker E
  TITLE     Mb- and FnCpf1 nucleases are active in mammalian cells: activities 
            and PAM preferences of four wild-type Cpf1 nucleases and of their 
            altered PAM specificity variants.
  JOURNAL   Nucleic Acids Res. 2018 Sep 20. pii: 5103950. doi: 
            10.1093/nar/gky815.
  PUBMED    30239882
REFERENCE   2  (bases 1 to 9722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Sep 20. pii: 5103950. doi: 10.1093/nar/gky815."
COMMENT     Sequence Label: pTE3328
FEATURES             Location/Qualifiers
     source          1..9722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        complement(213..461)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(271..290)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(441..461)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     enhancer        524..827
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        828..1031
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     982..1002
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1076..1095
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1076..1094
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1130..1139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1142..1162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1187..5104
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPRCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      4824..4833
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5105..5152
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5159..5239
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5313..5330)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5319..5543
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5589..6017
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5676..5695)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5886..5907
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6026..6046)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6031..6360
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6211..6346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6273..6292
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6427..7221
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6481..6500)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7091..7110
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7395..7516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7432..7451)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7486..7505
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7565..7581)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7565..7581)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7578..7600)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7589..7605
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7613..7619,7620..7637,7638..7643))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7658..7679
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7796..7813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7967..8555)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8047..8066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8726..9517,9518..9586))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9349..9368
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9587..9691)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattaaaaa aggagacggg
      181 atcccgtctc catctacaag agtagaaatt acggtgtttc gtcctttcca caagatatat
      241 aaagccaaga aatcgaaata ctttcaagtt acggtaagca tatgatagtc cattttaaaa
      301 cataatttta aaactgcaaa ctacccaaga aattattact ttctacgtca cgtattttgt
      361 actaatatct ttgtgtttac agtcaaatta attccaatta tctctctaac agccttgtat
      421 cgtatatgca aatatgaagg aatcatggga aataggccct cactagttat taatagtaat
      481 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      541 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      601 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      661 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      721 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      781 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      841 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      901 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      961 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1021 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata
     1081 cgactcacta tagggagacc caagctggct agcgtttaaa cttaagcttg ccaccatggc
     1141 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccacac agttcgaggg
     1201 ctttaccaac ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa
     1261 gaccctgaag cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca
     1321 ctacaaggag ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct
     1381 gcagctggtg cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga
     1441 gaaaaccgag gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat
     1501 ccacgactac ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc
     1561 cgagatctac aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct
     1621 gggcaccgtg accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac
     1681 aacctacttc tccggctttt atgagaacag gaagaacgtg ttcagcgccg aggatatcag
     1741 cacagccatc ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca
     1801 catcttcaca cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa
     1861 gaaggccatc ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta
     1921 taaccagctg ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc
     1981 tcgggaggca ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca
     2041 gaagaatgat gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt
     2101 taagcagatc ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga
     2161 cgaggaagtg atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct
     2221 ggagacagcc gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat
     2281 cagccacaag aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag
     2341 gaatgccctg tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa
     2401 ggagaaggtg cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc
     2461 cgcaggcaag gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc
     2521 acacgccgcc ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat
     2581 cctgaagtct cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt
     2641 ggatgagtcc aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga
     2701 gatggagcct tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta
     2761 ctccgtggag aagttcaagc tgaactttca gatgcctaca ctggcccggg gctgggacgt
     2821 gaataaggag aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg
     2881 catcatgcca aagcagaagg gcaggtataa ggccctcagc ttcgagccca cagagaaaac
     2941 cagcgagggc tttgataaga tgtactatga ctactttccg gatgccgcca agatgatccc
     3001 acggtgcagc acccagctga aggcggtgac cgcccacttt cagacccaca caacccccat
     3061 cctgctgtcc aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa
     3121 caatcctgag aaggagccaa agaagtttca gacagcgtac gccaagaaaa ccggcgacca
     3181 gaagggctac agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa
     3241 gtataccaag acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga
     3301 cctgggcgag tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat
     3361 cgccgagaag gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta
     3421 taacaaggac tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac
     3481 cggtctgttt tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga
     3541 gctgttctac cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat
     3601 gctgaacaag aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct
     3661 gtacgactat gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct
     3721 gcccaacgtg atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag
     3781 cgacaagttc tttttccacg tgcctatcac actgaactat caggccgcca attccccatc
     3841 taagttcaac cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg
     3901 catcgatcgg ggcgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat
     3961 cctggagcag cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa
     4021 cagggagaag gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga
     4081 tctgaagcag ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta
     4141 ccaggccgtg gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat
     4201 cgccgagaag gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct
     4261 ggtgctgaag gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac
     4321 agaccagttc acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc
     4381 tgccccatat acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa
     4441 aaccatcaag aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta
     4501 cgacgtgaaa accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca
     4561 gaggggcctg cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca
     4621 gtttgacgcc aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa
     4681 tcacagattc accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct
     4741 ggaggagaag ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa
     4801 tgacgattct cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg
     4861 gaactccaat gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg
     4921 cgtgtgcttc gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg
     4981 cgcctaccac atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga
     5041 tctgaagctg cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg
     5101 caacaaaagg ccggcggcca cgaaaaaggc cggccaggca aaaaagaaaa agggatccta
     5161 cccatacgat gttccagatt acgcttatcc ctacgacgtg cctgattatg catacccata
     5221 tgatgtcccc gactatgcct aagaattctg cagatatcca gcacagtggc ggccgctcga
     5281 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     5341 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     5401 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     5461 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     5521 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     5581 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5641 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5701 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5761 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5821 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5881 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5941 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6001 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     6061 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     6121 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6181 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     6241 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     6301 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     6361 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     6421 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6481 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6541 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6601 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6661 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6721 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6781 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6841 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6901 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     6961 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     7021 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7081 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7141 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7201 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     7261 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     7321 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     7381 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7441 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7501 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     7561 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     7621 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     7681 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     7741 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     7801 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     7861 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     7921 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7981 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8041 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8101 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8161 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8221 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8281 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8341 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8401 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8461 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8521 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8581 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8641 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     8701 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     8761 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     8821 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     8881 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     8941 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9001 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9061 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9121 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9181 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9241 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9301 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9361 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9421 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9481 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9541 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9601 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9661 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     9721 tc
//
