LOCUS       sequence-207280-        4492 bp    DNA     circular SYN 14-APR-2025
DEFINITION  cGAL-C driver under the control of C. elegans rab-3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
  TITLE     Split cGAL, an intersectional strategy using a split intein for 
            refined spatiotemporal transgene control in Caenorhabditiselegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115.
  PUBMED    29581308
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115."
COMMENT     Sequence Label: pHW522 (Prab-3::cGAL-C::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4492
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1514..1663
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     promoter        2201..2305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2306..2374,2375..3166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2524..2543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3337..3925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3826..3845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4079..4096
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4213..4234
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4249..4254,4255..4272,4273..4279)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4287..4303
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4292..4314
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4311..4327
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4311..4327
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gatcttcaga tgggagcagt ggactgtcta tttgatattc ttaaaatgta ctctataaaa
       61 gatgaaactg taatgaccaa ttccctacat aaatattttt aactaattgg tcggatattt
      121 ggggatcaga gaagtacttg tttttgcgat attaataatg atatattttt ctagtaaaaa
      181 aatgttaagc gatttgtgaa taattaagtt ggcttgaaaa agattttttg atagctatgt
      241 attttgttaa tttaaatcag attttaaaac tttattattg actatttata tatattatat
      301 attatatatt ttcatggttt aatcaaaatc tattaaatta tttctaaaat ctctaaatat
      361 cgaattctct aaaaagcttc atcaacaaaa gcatttcaat tatctaacac aatgagaata
      421 attcaagaac tgaaatagaa agaaatctag aaaaaaaaga gagtgaggtg ccctattaaa
      481 tcggtgctct cctctctaac agaagcgcct acacgcaccc aagagagctc tttttctttc
      541 ttttttcgga gcacacatac acgcatacac tcctcagaag tgtatagaga aaatgggaga
      601 agaagcgaaa gaagacgaag aagcggttgt gatgtatgca tcgggcgttt ctatctctct
      661 ccgtgagcaa cgagctagtc aacccatcga tccatttttg tgagcacaca cagagagaga
      721 ctcaaacaca ctcacagctt cacactgagc tccaagagct ctgtagagag aagccagagt
      781 atgctttcct cgattttcct aacgtcttct tcttctgctt ttttcttttt cccaacgagt
      841 tgtagtccac taacttcaat ttttcctcca gagaaagagt tttagagaga cagctgataa
      901 atacactaga gaaataaaaa gaatgataaa aatttgctat tttaatcagt tccctctcgt
      961 ttctctaagt tttgttgttg ggttgggaaa ttaaaattgg aaaatggaaa taacgttttt
     1021 ttctgtctaa tcttgaattc tccagacatt tcttatttcc ttttttgttg caagtttttc
     1081 ttccttctct aatttttaat caaagatatc tcgctatgaa ctttgaattt ttgatatttt
     1141 gattctataa atacataaag taatctaaat gctcttttaa aataaatcta cagtagccct
     1201 attttcaggg cgcgcctcta gaggatcccc gggattggcc aaaggaccca aaggtatgtt
     1261 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta ggatgggccc
     1321 taaaaagaag cgtaaagcta gcatgatgtt gaagaagatt ctcaaaattg aagaactgga
     1381 tgagcgtgag ctcatcgaca tcgaggtctc cggaaaccac ctcttctacg ccaacgacat
     1441 cctcacccac aactcctcct ccgatgtact gcagtctggt ggcggagggg ctcctagggg
     1501 ttccggacgg gctgacgcat tggacgattt tgatctggat atgctgggaa gtgacgccct
     1561 cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg acctcgacat
     1621 gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaact aaggtaccga
     1681 attcgctagc cggccataca agtaatccgg atgatcgacg ccaacgtcgt tgaattttca
     1741 aattttaaat actgaatatt tgtttttttt cctattattt atttattctc tttgtgtttt
     1801 ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt ttctctttcc
     1861 gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt tatttatctt
     1921 ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac acacaaaagg
     1981 atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag aaatatggag
     2041 gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag taaaaaataa
     2101 gcaaagaaat taggcgaacg atgagaattg tcctcgcttg ggcccaaaag gccttttttt
     2161 tttggcgccg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2221 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2281 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2341 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2401 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2461 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2521 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2581 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2641 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2701 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2761 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2821 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2881 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2941 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3001 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3061 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3121 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3181 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3241 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3301 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3361 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3421 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3481 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3541 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3601 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3661 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3721 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3781 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3841 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3901 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3961 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4021 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4081 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     4141 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     4201 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     4261 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4321 ctatgaccat gattacgcca agctgtaagt ttaaacatga tcttactaac taactattct
     4381 catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg atacgctaac
     4441 aacttggaaa tgaaataagc ttgcatgcgc ggccgcactg actgggccgg cc
//
