LOCUS       sequence-207282-        4313 bp    DNA     circular SYN 14-APR-2025
DEFINITION  cGAL-N driver under the control of C. elegans eft-3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Wang H, Liu J, Yuet KP, Hill AJ, Sternberg PW
  TITLE     Split cGAL, an intersectional strategy using a split intein for 
            refined spatiotemporal transgene control in Caenorhabditiselegans.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115.
  PUBMED    29581308
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Mar 26. pii: 1720063115. doi: 
            10.1073/pnas.1720063115."
COMMENT     Sequence Label: pHW533 (Peft-3::cGAL-N::let-858 3'UTR)
FEATURES             Location/Qualifiers
     source          1..4313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..600
                     /gene="eft-3"
                     /label=eft-3 promoter
                     /note="promoter for the C. elegans elongation factor 
                     1-alpha gene (Mitrovich and Anderson, 2000)"
     promoter        2022..2126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2127..2195,2196..2987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2345..2364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3158..3746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3647..3666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3900..3917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4034..4055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4070..4075,4076..4093,4094..4100)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4108..4124
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4113..4135
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4132..4148
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4132..4148
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcacctttgg tcttttattg tcaacttcca ttggttcttc cattgtttct gttaaattaa
       61 tgaatttttc ataaaataaa gacattatac aatataaaaa tgaagaattt attgaaaata
      121 aactgccaga gagaaaaagt atgcaacact cccgccgaga gtgtttgaaa tggtgtacgg
      181 tacattttcg tgctaggagt tagatgtgca ggcagcaacg agagggggag agattttttt
      241 gggccttgtg aaattaacgt gagttttctg gtcatctgac taatcatgtt ggttttttgt
      301 tggtttattt tgtttttatc tttgttttta tccagattag gaaatttaaa ttttatgaat
      361 ttataatgag gtcaaacatt cagtcccagc gtttttcctg ttctcactgt ttagtcgaat
      421 ttttatttta ggctttcaac aaatgttcta actgtcttat ttgtgacctc actttttata
      481 tttttttaat ttttaaaaat attagaagtt tctaggataa ttttttcgac ttttattctc
      541 tctaccgtcc gcactcttct tacttttaaa ttaaattgtt tttttttcag ttgggaaaca
      601 ctttgctcag gcgcgcctct agaggatccc cgggattggc caaaggaccc aaaggtatgt
      661 ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct aggatgggcc
      721 ctaaaaagaa gcgtaaagct agcatgaagc tgttgtcttc aatggaacaa gcttgcgaca
      781 tatgccggtt aaaaaaactc aggtgctcga aggaaaagcc caagtgtgcc aagtgtctga
      841 agaacaactc ggagtgccgt tattctccca aggctatgag gtccccactg accagggcgc
      901 atctgacaga ggtagaatca aggttggaaa aattggaaga tttgtttctg ctcatgtttc
      961 ctcgggaaaa cctggacagc attttgaaca tggactcttt ggacgaagtg aaagtaatgt
     1021 tgaaacaatt atatatgcag gactatggtg acacggatgc tgctatggat agcttgaccc
     1081 ctatggaaaa tggcatgtct gaagggccga gaaaacacat gaagactgcg acatcatcgc
     1141 caaaagtgga tgaagatgga agccaaagtc agttgagtgt atcgccggaa ttcccgctgc
     1201 agtctggtgg cggaggggct cctaggtgcc tcgacctcaa gacccaagtc caaaccccac
     1261 aaggaatgaa ggagatctcc aacatccaag tcggagacct cgtcctctcc aacaccggat
     1321 acaacgaggt cctcaacgtc ttcccaaagt ccaagaagaa gtcctacaag atcaccctcg
     1381 aggacggaaa ggagatcatc tgctccgagg agcacctctt cccaacccaa accggagaga
     1441 tgaacatctc cggaggactc aaggagggaa tgtgcctcta cgtcaaggag taaggtaccg
     1501 aattcgctag ccggccatac aagtaatccg gatgatcgac gccaacgtcg ttgaattttc
     1561 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
     1621 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc
     1681 cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct
     1741 tctcgctttc atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag
     1801 gatgatggaa atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga
     1861 ggaaggttta aaagaaaaca taaaaatata tagaggagga aggaaaacta gtaaaaaata
     1921 agcaaagaaa ttaggcgaac gatgagaatt gtcctcgctt gggcccaaaa ggcctttttt
     1981 ttttggcgcc gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2041 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2101 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2161 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     2221 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     2281 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2341 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2401 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2461 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2521 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2581 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2641 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2701 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2761 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2821 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2881 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2941 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     3001 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     3061 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     3121 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     3181 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     3241 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     3301 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     3361 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     3421 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     3481 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     3541 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     3601 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     3661 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     3721 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     3781 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3841 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3901 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3961 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     4021 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     4081 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     4141 gctatgacca tgattacgcc aagctgtaag tttaaacatg atcttactaa ctaactattc
     4201 tcatttaaat tttcagagct taaaaatggc tgaaatcact cacaacgatg gatacgctaa
     4261 caacttggaa atgaaataag cttgcatgcg cggccgcact gactgggccg gcc
//
