LOCUS       sequence-207290-        2892 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bacterial expression of mutant human alpha synuclein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2892)
  TITLE     Lashuel Lab plasmids
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pT7-7 asyn K32R
FEATURES             Location/Qualifiers
     source          1..2892
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      25..613
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     514..533
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     767..784
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     1019..1041
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1106..1125
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1106..1124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1156..1178
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             complement(join(1737..2528,2529..2597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2360..2379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2598..2702)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2770..2788
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
       61 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
      181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      661 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      721 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      781 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      841 acaccgcata ggaagatctt ccggaagatc ttcctatggt gcactctcag tacaatctgc
      901 tctgatgcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
      961 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     1021 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcccagcgat
     1081 tcgaacttct gatagacttc gaaattaata cgactcacta tagggagacc acaacggttt
     1141 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgga tgtattcatg
     1201 aaaggacttt caaaggccaa ggagggagtt gtggctgctg ctgagaaaac caaacagggt
     1261 gtggcagaag cagcaggaag gacaaaagag ggtgttctct atgtaggctc caaaaccaag
     1321 gagggagtgg tgcatggtgt ggcaacagtg gctgagaaga ccaaagagca agtgacaaat
     1381 gttggaggag cagtggtgac gggtgtgaca gcagtagccc agaagacagt ggagggagca
     1441 gggagcattg cagcagccac tggctttgtc aaaaaggacc agttgggcaa gaatgaagaa
     1501 ggagccccac aggaaggaat tctggaagat atgcctgtgg atcctgacaa tgaggcttat
     1561 gaaatgcctt ctgaggaagg gtatcaagac tacgaacctg aagcctaaga aatatctttg
     1621 ctcccagttt cttgagatct gctgacagat gttccatcca agcttatcga tgataagctg
     1681 tcaaacatga gaattaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     1741 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     1801 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1861 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     1921 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1981 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2041 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2101 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2161 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2221 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2281 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2341 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2401 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2461 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     2521 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     2581 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     2641 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     2701 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     2761 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaataaaa ggatctaggt
     2821 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2881 agcgtcagac cc
//
