LOCUS       sequence-207373-        3813 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Synthetic biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Gantner J, Ordon J, Ilse T, Kretschmer C, Gruetzner R, Lofke C, 
            Dagdas Y, Burstenbinder K, Marillonnet S, Stuttmann J
  TITLE     Peripheral infrastructure vectors and an extended set of plant parts
            for the Modular Cloning system.
  JOURNAL   PLoS One. 2018 May 30;13(5):e0197185. doi: 
            10.1371/journal.pone.0197185. eCollection 2018.
  PUBMED    29847550
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2018-05-30"; volume: "13(5):e0197185. doi"; pages: " 
            10.1371/journal.pone.0197185. eCollection 2018"
COMMENT     Sequence Label: pTEI037
FEATURES             Location/Qualifiers
     source          1..3813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     32..51
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     285..302
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             join(1224..1226,1227..1937)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1266..1287)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1527..1546)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1874..1895
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1982..2000)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2068..2172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2394..3185
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3356..3813,1..131)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
       61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      181 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      241 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      301 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      361 aatcactctg tggtctcacc atgattcagc aagccactgc aggagtctca caagacactt
      421 cggaaaatcc taacaaaaca atagttcctg ctgcattacc acagctcacc cctaccttgg
      481 tgtcactgct ggaggtgatt gaacccgagg tgttgtatgc aggatatgat agctctgttc
      541 cagattcagc atggagaatt atgaccacac tcaacatgtt aggtgggcgt caagtgattg
      601 cagcagtgaa atgggcaaag gcgataccag gcttcagaaa cttacacctg gatgaccaaa
      661 tgaccctgct acagtactca tggatgtttc tcatggcatt tgccctgggt tggagatcat
      721 acagacaatc aagtggaaac ctgctctgct ttgctcctga tctgattatt aatgagcaga
      781 gaatgtctct accctgcatg tatgaccaat gtaaacacat gctgtttgtc tcctctgaat
      841 tacaaagatt gcaggtatcc tatgaagagt atctctgtat gaaaacctta ctgcttctct
      901 cctcagttcc taaggaaggt ctgaagagcc aagagttatt tgatgagatt cgaatgactt
      961 atatcaaaga gctaggaaaa gccatcgtca aaagggaagg gaactccagt cagaactggc
     1021 aacggtttta ccaactgaca aagcttctgg actccatgca tgaggtggtt gagaatctcc
     1081 ttacctactg cttccagaca tttttggata agaccatgag tattgaattc ccagagatgt
     1141 tagctgaaat catcactaat cagataccaa aatattcaaa tggaaatatc aaaaagcttc
     1201 tgtttcatca aaaagcttct gccgtgagca agggcgagga gctgttcacc ggggtggtgc
     1261 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1321 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1381 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     1441 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1501 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1561 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1621 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     1681 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     1741 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1801 tgctgctgcc cgacaaccac tacctgagta ctcagtccaa gctgagcaaa gaccccaacg
     1861 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1921 tggacgagct gtacaaatct gcaatgtgag accacgaagt ggctcttcag tggacgaaag
     1981 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     2041 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     2101 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     2161 aaaaggaaga gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt
     2221 aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta
     2281 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     2341 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg
     2401 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     2461 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     2521 agccacacag cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     2581 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     2641 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     2701 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     2761 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     2821 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     2881 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     2941 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     3001 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     3061 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     3121 cagatcagtt ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca
     3181 aataactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     3241 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     3301 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3361 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3421 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3481 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3541 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3601 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3661 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3721 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3781 aggcggacag gtatccggta agcggcaggg tcg
//
