LOCUS       sequence-207528-         691 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  TITLE     SGC Oxford April 2018 plasmids
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP14 (PARP14A-c013)
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatgg cagcagcggg gcctgggaaa acatcatggg aaaaaggaag cctggtgtcc
      121 ccgggaggcc tgcagatgct gttggtgaaa gagggtgtgc agaatgctaa gaccgatgtt
      181 gttgtcaact ccgttccctt ggatctcgtg cttagtagag ggcctctttc ttcgtccctc
      241 ttggaaaaag ctggaccaga gctccaggag gaattggaca cagttggaca aggggtggct
      301 gtcagcatgg gcacagtgct caaaaccagc agctggaatc tggactgtcg ctatgtgctt
      361 cacgtggtag ctccggagtg gagaaatggt agcacatctt cactcaagat aatggaagac
      421 ataatcagag aatgtatgga gatcactgag agcttgtcct taaaatcaat tgcatttcca
      481 gcaataggaa caggaaactt gggatttcct aaaaacatat tcgctgaatt aatcatttca
      541 gaggtgttca gttttagtag ctcgaatcag ctgagtactt tacaagaggt tcactttctg
      601 ctgcacccga gtgatcatga aaatattcag gcattttcag atgaatttgc cagaagggct
      661 aattgacagt aaaggtggat acggatccga a
//
