LOCUS       sequence-207999-       14313 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Arabidopsis transformation, expresses HDT2/GFP fusion with HDT2 
            promoter to study expression pattern in roots.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14313)
  AUTHORS   Li H, Torres-Garcia J, Latrasse D, Benhamed M, Schilderink S, Zhou 
            W, Kulikova O, Hirt H, Bisseling T
  TITLE     Plant-Specific Histone Deacetylases HDT1/2 Regulate GIBBERELLIN 
            2-OXIDASE2 Expression to Control Arabidopsis Root Meristem Cell 
            Number.
  JOURNAL   Plant Cell. 2017 Sep;29(9):2183-2196. doi: 10.1105/tpc.17.00366. 
            Epub 2017 Aug 30.
  PUBMED    28855334
REFERENCE   2  (bases 1 to 14313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.17.00366"; journalName: "Plant Cell"; date: "2017-09"; 
            volume: "29"; issue: "9"; pages: "2183-2196"
COMMENT     Sequence Label: pHDT2:HDT2-GFP
FEATURES             Location/Qualifiers
     source          1..14313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    98..122
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     189..205
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     189..205
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    225..245
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    306..330
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2777..2801)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2809..2811,2812..2814,2815..3525)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2809..2811,2812..2814,2815..3525)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2854..2875)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3115..3134)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3462..3483
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3767..3787)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(3987..4008)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4199..4218
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(4500..4752)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6712..6891
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6933..7484
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      7504..7756
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7849..7873
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8394..9185
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9431..10019
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9920..9939
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10173..10190
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10205..10345
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10271..10290)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10431..10453
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10689..10883
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10949..12022)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12451..13080)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac gcccttttaa
       61 atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa tatatcctgt
      121 caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatccaag ctcaagctaa
      181 gcttcacaca ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa
      241 agttggccag agaccaagct tctcgagggt ctctcgcgcc aaagggcgcg cctaacccgg
      301 gtaccacaag tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc gtctctcttc
      361 ttctgtagca tttagctcga gttcttatgc acttcaaggc ttcttttttt ggtggacaag
      421 atcatttgaa tttttgttgt attatctaat aaatatttta ccgaaactat ttaaacgaaa
      481 aaactgaaga tttatttctg ttttattttt cataatcata tgatttagag aatgagaatc
      541 gaataatctc agaatattta agttaatatt tcatttatca atttagaatc tttaccaaat
      601 atgcaaataa ttttacatta acgcaaatat tccaaaataa aggaagcgtt acgaaattaa
      661 gggcaaaacg acgccgtgta gactctcgaa gaaccctaaa gagacttctt aaacctcatt
      721 cgattataaa atagccccaa acccactgcc tctttcactg caatttctct ctttcgcttc
      781 taaaaaaaaa cctaacaacc tctcttctct cttcctcgtt caacaacaat ggagttctgg
      841 ggtaagattc tttatttatt cctcttatat cttccgtcta ctgtttctat tttccacgtt
      901 ccgagaattt tgatcgattg ttcatataag atcgtattta gctgtatttc gtgtatatgt
      961 tcataccttg ttgcctttgt agcttattta gggtttaggt ttcacataga gcttggtttc
     1021 ggtatcagct ttgattatgt gtagatgttt tacaagttga tgagtgactc atgtttgatt
     1081 tctttgtata atgttattta ggagttgcgg tgacaccaaa aaacgctact aaggtgactc
     1141 ctgaagaaga cagccttgtc cacatttctc aggttatttt cacacttcca aactgtataa
     1201 agtttttagg atttagctta atacagtctc tttatgtttt ggttctcttt ctttatgtgt
     1261 aggcttcact tgactgcaca gtgaaatctg gagaatctgt ggttttgagt gtgactgttg
     1321 gtggggctaa acttgttatt ggaacacttt cacaagacaa gttccctcag attagctttg
     1381 atttggtttt tgataaagag tttgagcttt cacacagcgg taccaaagca aatgttcatt
     1441 tcattggcta caaatccccc aacatcgagc aatatccttt ttttgataga tttgactagg
     1501 tgttctttgt ctctatattt aagactggaa gatactagaa ggtttagcta caatgtctta
     1561 ttggttgtgt attccttaat tcttgttgat tgagtttcca cggatgactt cactagttcg
     1621 gatgatgagg atgttcctga agctgttcct gctcctgccc ctactgctgt tactgccaac
     1681 ggtatgtgtt gttgatgcta atattgtatt tgtttctttt attgaaaagt tgttatctca
     1741 tatgtcaatt tcttttgaca tttcaggaaa tgctggagca gctgttgtca aggctgacac
     1801 aaagccaaag gccaaacctg ccgaagtgaa gcctgcagaa gagaagcctg aatcagacga
     1861 ggaagatgag tctgatgatg aagatgagtc tgaagaggat gatgactctg agaaaggagt
     1921 gagtttttgt agctgtttat gatatctgtt tttctatcta gagtactcat atatacagtc
     1981 aggagaagat gagtagttgg ttgataaatt atatggtgat gtttttttgt agatggatgt
     2041 tgatgaagat gactcagatg atgacgagga ggaggattct gaggatgaag aagaggagga
     2101 gactcctaag aaggtagata cattgtctat caagcagttc tttgtttact ttacttttta
     2161 cataatttaa ttctccttct gaccttaatt gtatcatatg atttgtgtta aagcctgagc
     2221 caatcaacaa gaagaggcca aatgaatctg tatccaaaac acccgtctct ggaaagaagg
     2281 caaaaccagc agcagcacca gcttctactc ctcagaagac aggtgaaaag atgaaactct
     2341 ctttgttttt gttccttaga agcattagat agttaaataa tgagaaagct ttcttagtaa
     2401 tttgttgtat actctgttgt attctgacga aagagatatc ttcacattgt gttgttggta
     2461 cagaagagaa gaagaaagga ggacacaccg ccacaccaca cccagctaag aagggtggaa
     2521 agtctcctgt gaatgctaac cagagcccca agtctggagg tcaatcatcc ggtggtaaca
     2581 acaacaagaa gtgagttgtc tctttcttta tctttctcat agcccaaaga tatgagtgaa
     2641 aatgttaatt ggtaatgatg tgtaatggta ttgtgtgtgt acaggccatt caactcaggc
     2701 aaacaatttg gtggttccaa caacaagggt tctaacaagg gcaagggaaa gggtagagct
     2761 aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ggcgcgccat ggtgagcaag
     2821 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     2881 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     2941 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3001 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3061 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3121 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3181 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3241 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3301 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3361 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3421 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3481 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggtac ctaaccgcgg
     3541 ccatgctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt
     3601 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc
     3661 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     3721 atcaataaaa tttctaattc ctaaaaccaa aatccagtga tccggacaac tttattatac
     3781 atagttgata attcactggc cgtcgtgacg tcgggcccat cgatggcgcg ctgattgccc
     3841 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
     3901 cgaaaatcct gtttgatggt ggttccgaaa tcggcaaaat cccttataaa tcaaaagaat
     3961 agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4021 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4081 catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4141 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4201 ggaagaaagc gaaaggagcg ggcgccattc aggccgatag cagcaccgta atcagtagcg
     4261 acagaatcaa gtttgccttt agcgtcagac tgtagcgcgt tttcatcggc attttcggtc
     4321 atagccccct tattagcgtt tgccatcttt tcataatcaa aatcaccgga accagagcca
     4381 ccaccggaac cgcctccctc agagccgcca ccctcagaac cgccaccctc agagccacca
     4441 ccctcagagc cgccaccaga accaccacca gagccgccgc cagcattgac aggaggcccg
     4501 atctagtaac atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt
     4561 gttttctatc gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat
     4621 aaataacgtc atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta
     4681 tatgataatc atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat
     4741 gtttgaacga tcggggatcc gctttacttg tacataagga acagatggtg gcgtccctcg
     4801 gttctttcat actgctcaac gattgtatag tcttcgttgt ggcttgttat atccagtttg
     4861 gagtcaacat agtagtaccc tggtagctgc acaggcttct ttgccatgta aatacttttg
     4921 aattcaacta ggtaatgacc accgtctttc agcttcagag ccttatgaat ctctcctttc
     4981 aacacgccat cacgaggata caaacgctca gtgctggctt cccagcccat tgtcttcttt
     5041 tgcataacag gtccatcgga aggaaagttc acgccaatga acttgacctt gtagatgaaa
     5101 cagccatcct gcaaactgga atcctgggtt acagtaacga cgccaccgtc ttcaaagttc
     5161 atgacccttt cccatttaaa tccttcagga aatgacagct ttttatagtc tggtatgtcg
     5221 gcagggtgct tgacatatac cttgcttcca tactgaaatt gtggtgacaa aatatcccaa
     5281 gcaaatggca aaggtccccc cttggttacc ttaagcttta cggtattgtg gccttcgtat
     5341 ggcctcccct ctccttcgcc ttctatttca aactcgtgcc cattgaccgt tccttccatg
     5401 cgaaccttaa acctcatgaa ctccttgata acattcttgg aagaccccat ggggtatgtt
     5461 tttaatcttg tttgtattga tgagttttgg tttgagtaaa gagtgaagcg gatgagttaa
     5521 tttatagact ataaaggaga tttgcatggc gatcacgtgt aataatgcat gcacgcatgt
     5581 gattgtatgt gtgtgctgtg agagagaagc tcttaggtgt ttgaagggag tgacaagtgg
     5641 cgaagaaaaa caattctccg cggctgcatg ctatgtgtaa cgtgtagcta atgttctggc
     5701 atggcatctt atgaacgatt ctttttaaaa acaaggtaaa aacttaactt cataaaatta
     5761 aaaaaaagag cgtttactaa gttggtttaa aaggggatga gagtctataa attttggagg
     5821 tagtgccgtt gggaatataa attgggagct taatcagaat tatagaagtt aaagttgatt
     5881 tagtcacggt caatataaat tgggaatttg agtcaaaatc ttccaaattc ggaatccgtc
     5941 ttgttacacc cggtggatag gagccgaacg gtttgaaaat acttgaaatg tggatgcagg
     6001 tgcaggctgg tttaatttta tgttgaatgg atacatgtca atcgaatttg agttataggt
     6061 acacatttta ctctgatact aaaatgtaac atttgtctca agaatgggta ggtcatcctt
     6121 actcgacaac ggtatgccac ttcacttgaa cgtatatctc attcaaccca actctaacac
     6181 acatttcata cccatgtcta aaataaagaa aattgaagaa aaacgctcaa tagcgtcaat
     6241 ccttacaaat ataatgtaca tggcaacctc gcatgaaaat agtaataaag aggagtgaat
     6301 aatataaagt agtcactaat tggatgacaa agcaaatgga accatccgat agatgttttg
     6361 agaacaagag gtacatttgt atatatgcat tggttgatac atttctgcca tctgaattgt
     6421 ttcttcctca aagaagaagc attcagccaa tagtgaaatt taaaatgttg gaaaaatgag
     6481 gatgcagtca gaaagtccag aatataagtg gtaactggta actgaatact tgcctcagaa
     6541 gcaagataag aaacataagt ctttaaagga atcaatcacc gatgaagaga gctccgaatc
     6601 gaattcgagc tcggtaccgc gaattatcga tcatgagcgg agaattaagg gagtcacgtt
     6661 atgacccccg ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa
     6721 cgttgaagga gccactgagc cgcgggtttc tggagtttaa tgagctaagc acatacgtca
     6781 gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct
     6841 tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat
     6901 attcactctc aactcgatcg aggggatcta ccatgagccc agaacgacgc ccggccgaca
     6961 tccgccgtgc caccgaggcg gacatgccgg cggtctgcac catcgtcaac cactacatcg
     7021 agacaagcac ggtcaacttc cgtaccgagc cgcaggaacc gcaggagtgg acggacgacc
     7081 tcgtccgtct gcgggagcgc tatccctggc tcgtcgccga ggtggacggc gaggtcgccg
     7141 gcatcgccta cgcgggtccc tggaaggcac gcaacgccta cgactggacg gccgagtcga
     7201 ccgtgtacgt ctccccccgc caccagcgga cgggactggg ctccacgctc tacacccacc
     7261 tgctgaagtc cctggaggca cagggcttca agagcgtggt cgctgtcatc gggctgccca
     7321 acgacccgag cgtgcgcatg cacgaggcgc tcggatatgc cccccgcggc atgctgcggg
     7381 cggccggctt caagcacggg aactggcatg acgtgggttt ctggcagctg gacttcagcc
     7441 tgccggtgcc gccccgtccg gtcctgcccg tcaccgaaat ctgatgaccc ctagagtcaa
     7501 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     7561 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     7621 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     7681 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     7741 tctatgttac tagatcgacc ggcatgcaag ctgataattc aattcggcgt taattcagta
     7801 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
     7861 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
     7921 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
     7981 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
     8041 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
     8101 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
     8161 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     8221 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
     8281 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
     8341 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
     8401 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
     8461 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
     8521 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
     8581 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
     8641 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
     8701 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
     8761 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
     8821 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
     8881 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
     8941 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
     9001 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
     9061 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
     9121 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
     9181 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     9241 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     9301 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
     9361 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9541 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     9601 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9661 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9721 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9781 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9841 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9901 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     9961 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10021 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    10081 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    10141 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    10201 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
    10261 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    10321 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    10381 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    10441 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
    10501 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
    10561 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
    10621 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
    10681 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
    10741 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
    10801 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
    10861 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
    10921 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
    10981 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
    11041 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
    11101 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
    11161 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
    11221 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
    11281 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
    11341 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
    11401 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
    11461 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
    11521 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
    11581 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
    11641 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
    11701 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
    11761 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
    11821 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
    11881 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
    11941 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
    12001 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
    12061 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
    12121 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
    12181 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
    12241 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
    12301 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
    12361 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
    12421 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
    12481 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
    12541 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
    12601 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
    12661 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
    12721 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
    12781 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
    12841 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
    12901 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
    12961 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
    13021 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
    13081 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
    13141 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
    13201 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
    13261 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
    13321 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
    13381 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
    13441 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
    13501 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
    13561 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
    13621 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
    13681 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
    13741 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
    13801 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
    13861 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
    13921 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
    13981 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
    14041 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
    14101 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
    14161 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
    14221 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttaa agtactttaa
    14281 agtactttaa agtactttga tcccgagggg aac
//
