LOCUS       sequence-208028-        1086 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Kory N, Wyant GA, Prakash G, Uit de Bos J, Bottanelli F, Pacold ME, 
            Chan SH, Lewis CA, Wang T, Keys HR, Guo YE, Sabatini DM
  TITLE     SFXN1 is a mitochondrial serine transporter required for one-carbon 
            metabolism.
  JOURNAL   Science. 2018 Nov 16;362(6416). pii: 362/6416/eaat9528. doi: 
            10.1126/science.aat9528.
  PUBMED    30442778
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-11-16"; volume: "362(6416). pii: 362/6416/eaat9528. 
            doi"; pages: " 10.1126/science.aat9528"
COMMENT     Sequence Label: pMXs_FLAG-Sfxn2 (CG6812)
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atggactaca aggaccacga cggtgactac aaggaccacg acatcgacta caaggacgac
       61 gacgacaagg ggcatggcac cggcagcacc ggcagcgaat tcatgtcaca agtttcaacc
      121 cttatagatg tcgataaacc cctcttcgat cttagcactt tcgcaggtag gttccagtac
      181 tttgcatgga tgactgaccc cagaactgtc gtggtgagtt cagacagact cttggaagcc
      241 aaagctatgg tagaaagata caggaaaggg gaccagtctc ccccactcaa accggaagag
      301 gttcattata atatgaaact gtataactcc gcttttcacc ccgatacagg agagctccaa
      361 aacttttgcg gtaggatgtc atttcaagtg cctggaggta tgctcattac tggcggaatg
      421 cttgctttct ataggactgt tcctgctgtc gtattgtggc agttcataaa tcaatcattc
      481 aacgcagtag taaactatac taatcgcaac gcgaacagtc ctacttcagt tactcaattg
      541 ggcgtcgctt atgtcagtgc gacgacttct gcgcttgttg cggcgattgg atgcaagaac
      601 tattggtcta agaaagcaac tcctttgttc cagaggttcg tgccctttgc cgctgttgct
      661 gccgcgaact tcgtcaatat acccttgatg aggcagaacg agataatcaa tggcatcgaa
      721 gtgaagaacg acgacggggt agtcgttggt cagagccgcc tggccgccat taaggggatt
      781 ggggaagttg tcgtgagcag aattgctatg gccgcgcccg gtatgttggt tctgcccctt
      841 atcatggagc gccttgagaa acttcctgca tataggcgca ttaagtggat aaatgcgccc
      901 ttccagacct tgttggtcgg ctgtttcctc tgtttcatgg ttcctacagc ttgtgcactg
      961 tttccccagc aatgctcact cgatacttct ataatgagga cgtttgaacc tgaactttat
     1021 gaggatttgg agaaaaagac ccaaggcaaa gtcccgaagc gcgtttactt caacaagggt
     1081 ctgtaa
//
