LOCUS       sequence-208258-        1154 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Bacterial expression of human USP30 (construct 13i).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Gersch M, Gladkova C, Schubert AF, Michel MA, Maslen S, Komander D
  TITLE     Mechanism and regulation of the Lys6-selective deubiquitinase USP30.
  JOURNAL   Nat Struct Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475.
  PUBMED    28945249
REFERENCE   2  (bases 1 to 1154)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2017 Sep 25. doi: 10.1038/nsmb.3475."
COMMENT     Sequence Label: Human USP30 (64-502, construct 13i, C77A, MG-3...
FEATURES             Location/Qualifiers
     source          1..1154
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     12..34
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             73..96
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1122..1139
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tggcctttgc agggctggca agccacgttt ggtggtggcg accatcctcc aaaatcggat
       61 ctgagcagcg gtctggaagt tctgtttcag ggcccgaaag gtctggttcc gggtctggtt
      121 aatctgggta ataccgcttt tatgaatagc ctgctgcagg gtctgagcgc atgtccggca
      181 tttattcgtt ggctggaaga attcaccagc cagtatagcc gtgatcagaa agaaccgcct
      241 agccatcagt atctgagcct gaccctgctg catctgctga aagcactgag ctgtcaagag
      301 gttaccgatg atgaagttct ggatgcaagc tgtctgctgg atgttctgcg tatgtatcgt
      361 tggcagatta gcagctttga agaacaggat gcacatgaac tgtttcatgt tattaccagc
      421 agcctggaag atgaacgtga tggttccggc tctcattgga aaagccagca tccgtttcat
      481 ggtcgtctga ccagtaatat ggtttgtaaa cattgcgaac atcagagtcc ggttcgtttt
      541 gatacctttg atagcctgag cctgtcaatt ccggcagcaa cctggggtca tccgctgacc
      601 ctggatcatt gtctgcatca tttcattagc agcgaaagcg ttcgtgatgt tgtttgtgat
      661 aactgcacca aaattgaagc aaaaggcacc ctgaatggtg aaaaagttga acatcagcgt
      721 accacctttg tgaaacagct gaaactgggt aaactgccgc agtgtctgtg tattcatctg
      781 cagcgtctgt cttggagcag ccatggtaca ccgctgaaac gtcatgaaca tgtgcagttt
      841 aatgaggacc tgagcatgga cgagtataaa taccactcca atgcgagtac ctacctgttt
      901 cgtctgatgg cagttgttgt tcatcatggt gatatgcata gcggtcattt tgttacctat
      961 cgtcgtagcc ctccgagcgc acgtaatccg ctgagcacca gtaatcagtg gctgtgggtt
     1021 agtgatgata ccgttcgtaa agcaagcctg caagaagttc tgagcagcag cgcatatctg
     1081 ctgttttacg agcgtgttta aagctttcta gaccatttaa acaccaccac caccaccact
     1141 gagatccggc tgct
//
