LOCUS       sequence-208492-        6936 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses the C. elegans pde-4 (isoform d) cDNA pan-neuronally.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Charlie NK, Thomure AM, Schade MA, Miller KG
  TITLE     The Dunce cAMP phosphodiesterase PDE-4 negatively regulates G 
            alpha(s)-dependent and G alpha(s)-independent cAMP pools in the 
            Caenorhabditis elegans synaptic signaling network.
  JOURNAL   Genetics. 2006 May;173(1):111-30. doi: 10.1534/genetics.105.054007. 
            Epub 2006 Apr 19.
  PUBMED    16624912
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.105.054007"; journalName: "Genetics"; date: 
            "2006-05"; volume: "173"; issue: "1"; pages: "111-30"
COMMENT     Sequence Label: KG#203
FEATURES             Location/Qualifiers
     source          1..6936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4376..4398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4498..4517
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4595..4613)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4681..4785
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4786..4854,4855..5646)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5004..5023)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5817..6405
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6306..6325
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6693..6714
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6729..6734,6735..6752,6753..6759)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6767..6783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6772..6794
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6791..6807
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6791..6807
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcaggatct tcagatggga gcagtggact gtctatttga tattcttaaa
       61 atgtactcta taaaagatga aactgtaatg accaattccc tacataaata tttttaacta
      121 attggtcgga tatttgggga tcagagaagt acttgttttt gcgatattaa taatgatata
      181 tttttctagt aaaaaaatgt taagcgattt gtgaataatt aagttggctt gaaaaagatt
      241 ttttgatagc tatgtatttt gttaatttaa atcagatttt aaaactttat tattgactat
      301 ttatatatat tatatattat atattttcat ggtttaatca aaatctatta aattatttct
      361 aaaatctcta aatatcgaat tctctaaaaa gcttcatcaa caaaagcatt tcaattatct
      421 aacacaatga gaataattca agaactgaaa tagaaagaaa tctagaaaaa aaagagagtg
      481 aggtgcccta ttaaatcggt gctctcctct ctaacagaag cgcctacacg cacccaagag
      541 agctcttttt ctttcttttt tcggagcaca catacacgca tacactcctc agaagtgtat
      601 agagaaaatg ggagaagaag cgaaagaaga cgaagaagcg gttgtgatgt atgcatcggg
      661 cgtttctatc tctctccgtg agcaacgagc tagtcaaccc atcgatccat ttttgtgagc
      721 acacacagag agagactcaa acacactcac agcttcacac tgagctccaa gagctctgta
      781 gagagaagcc agagtatgct ttcctcgatt ttcctaacgt cttcttcttc tgcttttttc
      841 tttttcccaa cgagttgtag tccactaact tcaatttttc ctccagagaa agagttttag
      901 agagacagct gataaataca ctagagaaat aaaaagaatg ataaaaattt gctattttaa
      961 tcagttccct ctcgtttctc taagttttgt tgttgggttg ggaaattaaa attggaaaat
     1021 ggaaataacg tttttttctg tctaatcttg aattctccag acatttctta tttccttttt
     1081 tgttgcaagt ttttcttcct tctctaattt ttaatcaaag atatctcgct atgaactttg
     1141 aatttttgat attttgattc tataaataca taaagtaatc taaatgctct tttaaaataa
     1201 atctacagta gccctatttt caggatcccc gggattggcc aaaggaccca aaggtatgtt
     1261 tcgaatgata ctaacataac atagaacatt ttcaggagga cccttggcta gcaaaaatgc
     1321 cacgaagacg cggctcgtcg tcgtcgtcgt cggcggcagg tggtagtggc ggcggcggag
     1381 gcttcggatt ctcgagcctc cgacgcgaac tccacttgca caacttcttt cgaacctctt
     1441 cgccgtcggc ctcgtcgacg tcgagaacac cgcccgcagc attaccaccg cgcacatcag
     1501 ctgttactat tccgggcagt aatcacaaat tgacctcttc cgcttcatct taccacccac
     1561 cacgcgagct taccgtgtcg acgttcagcg ccggctcggc aactgcagct gatggacttg
     1621 gaggtgctca tctaacgcca tcactttcat cttcggtaca tgcgagacgg gagtcgtttc
     1681 tctaccgtgc cagtgatgat cttcgagaag cgtcttcgtt acgaccagtc agtcgggcgt
     1741 cgtcaatcgc gtccaatgag cacggacacg gtgacgacct gattgtaacc ccgtttgctc
     1801 aactgcttgc cagtcttcga aacgttcgat ccaatctgat ttcgataacc aacatccaga
     1861 acagcgacga cagtcgacac gctaatcgaa gtgcgaaacg gccgcctttg cacaacattg
     1921 agcttccaga cgatgtggtg cattgtgcgc atgatacact cgaagaactc gattggtgtc
     1981 ttgatcagct ggaaacaatt caaactcatc gatcagtgtc tgagatggcc agcagtaagt
     2041 tccgcaagat gctcaacaaa gagttgtcac actttgctga atcgtccaaa tccggcactc
     2101 aggtctccaa gtttttgatc accacctaca tggacaagga agaggatgaa ccgtcgatcg
     2161 agatcgaagt gcccactgag gtacaaggcc cttctacatc tggaccaatg acactttcga
     2221 tactgaaaaa agctcaaaca gcagccatga ataagatctc aggagttcga aaacttcgtg
     2281 ctccatcaca tgacggccat gttcccgaat acggtgtaaa ctgtgcacgt gagatagctg
     2341 ttcatatgca acgactcgat gactggggac ctgatgtctt caaaatcgac gaactttcca
     2401 aaaatcactc gctcactgtc gttacatttt cattactccg ccagcggaat ctctttaaaa
     2461 cttttgaaat tcatcaatct actctggtca cctacttgct caatcttgaa catcactatc
     2521 gtaataatca ttatcacaat tttattcatg cagccgatgt tgcacagtca atgcacgttc
     2581 tgctcatgtc tcccgtcctt acagaagtct tcactgatct tgaagtatta gcagctatat
     2641 ttgccggtgc tgtccatgat gtagatcatc ctggattcac gaatcagtat ttgatcaact
     2701 caaacaatga attagcgatc atgtacaatg atgaaagtgt ccttgagcag catcatctgg
     2761 cagttgcatt caagcttttg caagattcaa actgtgattt ccttgcaaat ttgtccagaa
     2821 aacaacggct gcaatttcgc aagattgtca ttgatatggt acttgcaact gatatgtcta
     2881 agcacatgtc tcttcttgct gatctgaaaa caatggtcga agcaaagaaa gttgctggaa
     2941 acaatgtgat tgtgctggac aagtacaatg acaaaattca ggtactacaa tcgatgattc
     3001 atctcgctga tctctcaaat ccaactaagc caatcgaatt ataccaacaa tggaatcaaa
     3061 ggataatgga agaatactgg aggcagggag ataaagagaa agagcttgga cttgagatat
     3121 caccgatgtg cgatcgagga aatgtgacca ttgaaaaatc acaggtcgga ttcatcgact
     3181 acattgttca tccattatac gaaacatggg cagatcttgt ctatccagat gctcaaaata
     3241 tactcgatca actcgaagaa aatcgagaat ggtaccaatc acgaatacct gaagaacctg
     3301 acacagctcg tactgtaaca gaagatgacg agcacaaata gataccggtc agatatcact
     3361 cgaggagctc ccgagatctt cagcgctcag cattcgtaga attgtaagtt taaactttct
     3421 catactaact aacgcgctct atttaaattt tcagaattcc aactgagcgc cggtcgctac
     3481 cattaccaac ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa
     3541 actgagttct actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc
     3601 tgacttctaa gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca
     3661 ccccctattt ttgttattat caaaaaaact tcttcttaat ttctttgttt tttagcttct
     3721 tttaagtcac ctctaacaat gaaattgtgt agattcaaaa atagaattaa ttcgtaataa
     3781 aaagtcgaaa aaaattgtgc tccctccccc cattaataat aattctatcc caaaatctac
     3841 acaatgttct gtgtacactt cttatgtttt ttttacttct gataaatttt ttttgaaaca
     3901 tcatagaaaa aaccgcacac aaaatacctt atcatatgtt acgtttcagt ttatgaccgc
     3961 aatttttatt tcttcgcacg tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa
     4021 aagttttgga gtatttttgg aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc
     4081 tgcttttgct ttttgggggt ttcccctatt gtttgtcaag agtttcgagg acggcgtttt
     4141 tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa gaaggtttgg
     4201 gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag tagtgtctat
     4261 ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa ctgtttccta
     4321 ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     4381 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     4441 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     4501 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     4561 taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct atttttatag
     4621 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4681 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4741 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4801 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4861 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4921 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4981 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5041 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5101 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5161 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5221 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5281 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5341 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5401 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5461 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5521 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5581 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5641 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5701 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5761 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5821 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5881 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5941 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6001 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6061 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6121 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6181 accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga
     6241 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6301 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6361 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6421 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6481 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6541 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6601 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6661 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6721 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6781 caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt ttaaacatga
     6841 tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc
     6901 acaacgatgg atacgctaac aacttggaaa tgaaat
//
