LOCUS       sequence-208523-        8610 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Alpha satellite repeats targeting Spy sgRNA under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, 
            Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ
  TITLE     C-BERST: defining subnuclear proteomic landscapes at genomic 
            elements with dCas9-APEX2.
  JOURNAL   Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2.
            Epub 2018 May 7.
  PUBMED    29735996
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0006-2"; journalName: "Nat Methods"; date: 
            "2018-06"; volume: "15"; issue: "6"; pages: "433-436"
COMMENT     Sequence Label: pEJS887_pTetR-P2A-BFPnls/sgAlpha
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             263..443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    490..615
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1108..1341
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1526..1570
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1868..2116
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1868..1888
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2039..2058
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2270..2387
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2436..2946
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2865..2883
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     intron          2955..3527
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3535..3554
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        3582..3600
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3611..4231
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(3684..3703)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             4265..4321
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4337..5047
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGIYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     CDS             5048..5068
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     LTR             5164..5397
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5469..5590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5506..5525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5560..5579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5630..5765
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(5786..5804)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5814..5831)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5814..5830)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5823..5845)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5972..6427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6059..6078)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6269..6290
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6453..6557
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6558..6626,6627..7418)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6776..6795)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7589..8177
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8078..8097
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8331..8348
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8465..8486
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8501..8506,8507..8524,8525..8531)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8539..8555
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8544..8566
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8563..8579
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8563..8579
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     join(8598..8610,1..8)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        join(8600..8610,1..8)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
       61 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
      121 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
      181 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
      241 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
      301 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      361 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
      421 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
      481 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
      541 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
      601 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
      661 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
      721 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
      781 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
      841 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
      901 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
      961 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1021 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1081 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1141 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1201 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1261 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1321 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1381 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     1441 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     1501 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     1561 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     1621 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     1681 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     1741 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     1801 tcgattagtg aacggatctc gacggtatcg atcacgagac tagcctcgag cggccgcccc
     1861 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     1921 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     1981 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     2041 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     2101 gaaaggacga aacaccgaat ctgcaagtgg atattgttta agagctatgc tggaaacagc
     2161 atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     2221 ctttttttga attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa
     2281 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     2341 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     2401 gggacagcag agatccactt tggccgcggc tcgagggggt tggggttgcg ccttttccaa
     2461 ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg gaaacgcagc
     2521 ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac ccggatcttc
     2581 gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag tcgggaaggt
     2641 tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct cactagtacc
     2701 ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc
     2761 aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag
     2821 gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc
     2881 aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac cgacctctct
     2941 ccccaggggg atccgtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa
     3001 aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc
     3061 ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta ctctgttgac
     3121 aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta aactttagct
     3181 tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt aagtactttc
     3241 tctaatcact tttttttcaa ggcaatcagg gtatattata ttgtacttca gcacagtttt
     3301 agagaacaat tgttataatt aaatgataag gtagaatatt tctgcatata aattctggct
     3361 ggcgtggaaa tattcttatt ggtagaaaca actacatcct ggtcatcatc ctgcctttct
     3421 ctttatggtt acaatgatat acactgtttg agatgaggat aaaatactct gagtccaaac
     3481 cgggcccctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
     3541 tgctggttat tgtgctgtct catcattttg gcaaagaatt gtaatacgac tcactatagg
     3601 gcgaattgat atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct
     3661 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga
     3721 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat
     3781 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga
     3841 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc
     3901 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt
     3961 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt
     4021 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga
     4081 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt
     4141 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt
     4201 agaaaaacaa cttaaatgtg aaagtgggtc cgcgtacagc ggctcccggg agttcgctag
     4261 cggtgctact aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc
     4321 tggtagtgga acgcgtatgg tgtctaaggg cgaagagctg attaaggaga acatgcacat
     4381 gaagctgtac atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga
     4441 aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct
     4501 ccccttcgcc ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa
     4561 ccacacccag ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga
     4621 gagagtcacc acatacgaag atgggggcgt gctgaccgct acccaggaca ccagcctcca
     4681 ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc
     4741 tgtgatgcag aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga
     4801 cggcggcctg gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat
     4861 cgcaaacgcc aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg
     4921 catctactat gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt
     4981 cgagcagcac gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa
     5041 gcttaatcca aagaaaaagc ggaaagtgtg agtcgacctc gagggggggc ccggtacctt
     5101 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5161 gactggaagg gctaattcac tcccaacgaa gaaaagatct gctttttgct tgtactgggt
     5221 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     5281 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     5341 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     5401 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     5461 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5521 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5581 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     5641 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5701 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     5761 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     5821 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     5881 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5941 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     6001 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     6061 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     6121 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     6181 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     6241 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     6301 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     6361 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     6421 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6481 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6541 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6601 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6661 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6721 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6781 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     6841 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6901 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6961 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     7021 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     7081 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     7141 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     7201 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7261 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7321 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7381 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7441 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     7501 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     7561 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     7621 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     7681 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     7741 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     7801 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7861 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7921 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7981 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     8041 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     8101 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     8161 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     8221 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     8281 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     8341 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     8401 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     8461 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     8521 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     8581 atgattacgc caagcgcgca attaaccctc
//
