LOCUS       sequence-208835-        3408 bp    DNA     circular SYN 15-APR-2025
DEFINITION  In situ hybridization and RNAi studies in planarians.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Accorsi A, Williams MM, Ross EJ, Robb SMC, Elliott SA, Tu KC, 
            Sanchez Alvarado A
  TITLE     Hands-On Classroom Activities for Exploring Regeneration and Stem 
            Cell Biology with Planarians
  JOURNAL   The American Biology Teacher 79(3):208-223. Mar 2017.
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "The 
            American Biology Teacher 79(3):208-223. Mar 2017."
COMMENT     Sequence Label: Pmo_innexin-10
FEATURES             Location/Qualifiers
     source          1..3408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..46)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(200..788)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(280..299)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(935..1744)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1658..1677
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1736..1755
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     1857..1873
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1857..1873
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1878..1896
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1954..1972
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2842..2858)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2842..2858)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(2916..2934)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2939..2986
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2993..3010)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2993..3009)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3002..3024)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3073..3159
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3251..3278
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
       61 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      121 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      181 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      241 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      301 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      361 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      421 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      481 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      541 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      601 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
      661 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      721 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
      781 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgacgcgt
      841 aactcacgtt aagggatttt ggtcatgagc ttgcgccgtc ccgtcaagtc agcgtaatgc
      901 tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat
      961 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     1021 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     1081 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     1141 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     1201 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     1261 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     1321 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     1381 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     1441 ttccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     1501 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     1561 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     1621 acaagcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     1681 ataaatcagc atccatgttg gaatttaatc gcggcctcga cgtttcccgt tgaatatggc
     1741 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgatgata
     1801 tatttttatc ttgtgcaatg taacatcaga gattttgaga cacgggccag agctgccagg
     1861 aaacagctat gaccatgtaa tacgactcac tataggggat cctcaaaaaa cccctcaaga
     1921 cccgtttaga ggccccaagg ggttatgtgg aagtaatacg actcactata gggcgaattg
     1981 ggtaccgggc ccggccaatt ctacccggcg aggtatatct cccagagccg ccgaacgatg
     2041 tccgccgtga tcagatcacc ggcgttgatg aagatcatcc tcaccaggaa cacgccgtcg
     2101 tgacgcagga actctccaat gaagtggttc aacttctgtt tatcggagcg cgtgtagttg
     2161 tccttcatca tgaggaactt cttcacgaag tggctcctgt tactcaggaa tgccatacga
     2221 atgaaccaca cgggaatgct gatggccgtc acaacaccaa ccaggaacac ccaaaaccaa
     2281 agaaagacgt aaatcttctc attgagcata ttgattggga gggcacactg cgccacgtac
     2341 ttattgctgg ccccgaggtg gcgtatctcc ccgatgtaac agtaagtgac ccttgggaag
     2401 atgcccgtct ggtgccagtg gcgcccattg atcatatttg atagcaccgt gtacccaaag
     2461 gatgagatgg actcgttgaa gcccagaaac cgtttcatga tggtcagctg accaaatacg
     2521 ttgatgcaat acagacactt catcagcatg tagcacagca ctagccacgt gcccatgtgc
     2581 tttgagaata cgcagaagtt gcacatgttg tacaccttgc ggcgcagttc cgcgaccttg
     2641 ccctgccggt agtctcggtg ctggaagagc atgtcctcga tggtgcgcgc caatttgtca
     2701 acgacctccg tccgctcctt cggtgagttg ttggcagcct tgctggcctc gttgatgagg
     2761 aactcgaggt cggttcctgt cttattgtag cagatcagtt gccagatcgg gatggtaatg
     2821 ccgctagcca tggaaccggt ggatccacta gttctagagc ggccgccacc gcggtggagc
     2881 tcgaattcat cgatgatatc agatctgccg gtctccctat agtgagtcgt attacttcct
     2941 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctta agactggccg
     3001 tcgttttaca acgtcgtgac tgggaaaaca tccatgctag cgttaacgcg agagtaggga
     3061 actgccaggc atcaaataaa acgaaaggct cagtcggaag actgggcctt tcgttttatc
     3121 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     3181 gttgtgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
     3241 caaactaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttcct
     3301 ggctagcggt acgcgtatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcg
//
