LOCUS       sequence-208865-        5712 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses dLight1.5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Patriarchi T, Cho JR, Merten K, Howe MW, Marley A, Xiong WH, Folk 
            RW, Broussard GJ, Liang R, Jang MJ, Zhong H, Dombeck D, von Zastrow 
            M, Nimmerjahn A, Gradinaru V, Williams JT, Tian L
  TITLE     Ultrafast neuronal imaging of dopamine dynamics with designed 
            genetically encoded sensors.
  JOURNAL   Science. 2018 Jun 29;360(6396). pii: science.aat4422. doi: 
            10.1126/science.aat4422. Epub 2018 May 31.
  PUBMED    29853555
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2018-06-29"; volume: "360(6396). pii: science.aat4422. doi"; 
            pages: " 10.1126/science.aat4422. Epub 2018 May 31"
COMMENT     Sequence Label: pCMV-dLight1.5
FEATURES             Location/Qualifiers
     source          1..5712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2136..2257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2173..2192)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2227..2246
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2264..2719)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2401..2422)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2613..2632
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2746..2850
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2852..3209
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2852..3043
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2875..2895)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3060..3195
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3244..4038
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3244..4038
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3298..3317)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3908..3927
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4226..4245)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4270..4317
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4646..5234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5135..5154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5409..5712
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttggatccg gtacctgcgc gccatgaaga cgatcatcgc
      301 cctgagctac atcttctgcc tggtgttcgc cgactacaag gacgatgatg acgccatgga
      361 cccccttaac ctctcatggt acgacgatga tcttgagagg cagaactggt cccgaccatt
      421 caatgggtct gatggtaagg ctgaccggcc tcattacaat tattatgcga ccctgcttac
      481 tcttcttatc gctgtgatcg tattcggcaa cgtcttggtt tgcatggcag tctctaggga
      541 aaaagcgctc cagacgacaa ctaattactt gattgtgagt ctggctgtag ctgacttgct
      601 tgtggcgacc ctggtgatgc catgggtcgt atacttggaa gtcgttggcg agtggaagtt
      661 ttctaggatt cattgcgaca tatttgtaac tctggacgta atgatgtgta ctgcttccat
      721 tttgaacctc tgcgctatat ccattgacag gtacacggcg gttgctatgc cgatgcttta
      781 taatacccgg tattcaagca aaaggcgagt aactgtgatg ataagcattg tatgggtgct
      841 cagtttcaca attagctgcc ctctgctctt cggccttaac aacgcggatc aaaatgaatg
      901 catcatcgca aacccggctt ttgtggttta tagcagcatt gttagcttct atgtgccatt
      961 catagttacg ctccttgttt atataaaaat ttatatcgtg cttaggcgcc gccgaaaact
     1021 gagctcactc attaatgtat atatcaaagc tgataagcaa aaaaacggta tcaaggctaa
     1081 ttttaagatc agacataata tagaggatgg aggcgttcaa ctggcctacc actaccagca
     1141 aaacacgccg atcggggatg ggccagtact tctgccagat aaccattatc tctcagttca
     1201 aagcaaactc tctaaggacc ctaatgagaa acgagatcat atggttctgc tcgaattcgt
     1261 tacagccgcc ggtatcacac ttgggatgga cgagttgtat aagggtggaa caggagggtc
     1321 aatggtaagc aaaggcgagg agctgtttac gggggtcgtc ccgatacttg ttgaactcga
     1381 cggcgatgtc aacgggcaca aattctcagt gagtggcgag ggggaaggag acgccactta
     1441 tggaaaactg acattgaaat tcatatgtac gactgggaag ttgcctgtgc cttggcctac
     1501 gctcgttact acacttactt acggggtaca gtgtttcagt aggtatccag atcacatgaa
     1561 acagcacgat tttttcaaga gtgcaatgcc ggaaggatat atacaagaaa gaactatttt
     1621 ctttaaagat gacggcaact ataaaacgcg agcagaggtg aagtttgagg gcgatacctt
     1681 ggttaatagg atcgaactca aaggcataga cttcaaagaa gacggaaaca ttctgggtca
     1741 caaactggaa tacaacaatc atgaccaact gcagaaggaa aagaaggcca cgcaaatgtt
     1801 ggcaatcgtg ctcggcgtgt tcataatctg ctggcttcca ttttttataa cgcatatatt
     1861 gaacatacac tgtgattgca atattccacc agtcctgtat agtgcgttta cgtggttggg
     1921 ttatgtgaat tctgcggtta acccgatcat ttacaccacg ttcaacatag aattccgaaa
     1981 ggcattcctc aaaatattgc attgttagtg tacagcggcc gcgactctag atcataatca
     2041 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     2101 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     2161 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2221 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat
     2281 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     2341 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     2401 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     2461 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     2521 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     2581 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     2641 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     2701 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     2761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     2821 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg
     2881 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2941 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3001 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     3061 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3121 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3181 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt
     3241 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     3301 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     3361 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     3421 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     3481 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     3541 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     3601 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     3661 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     3721 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc
     3781 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     3841 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     3901 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     3961 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     4021 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     4081 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4141 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4201 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
     4261 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata
     4321 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     4381 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     4441 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata
     4501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     4561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     4621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     4681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     4741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     4801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     4861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     4921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     4981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     5341 ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
     5401 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
     5461 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
     5521 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
     5581 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
     5641 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     5701 gctattacca tg
//
