LOCUS       sequence-208960-       14503 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses FANCA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 14503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1316
FEATURES             Location/Qualifiers
     source          1..14503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4325..5503
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4556..5494
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5451..5471
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5520..5538
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5586..5612
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(10178..10195)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10405..10424
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      10580..10589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10592..11080
                     /codon_start=1
                     /product="mouse GPI-anchored cell surface glycoprotein of 
                     the immunoglobulin superfamily"
                     /label=Thy1.1
                     /note="This allele of Thy1 is also known as CD90.1."
                     /translation="MNPAISVALLLSVLQVSRGQKVTSLTACLVNQNLRLDCRHENNTK
                     DNSIQHEFSLTREKRKHVLSGTLGIPEHTYRSRVTLSNQPYIKVLTLANFTTKDEGDYF
                     CELRVSGANPMSSNKSISVYRDKLVKCGGISLLVQNTSWMLLLLLSLSLLQALDFISL"
     protein_bind    complement(11086..11110)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    11141..11729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11194..11214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11612..11623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        11780..12279
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11815..11838)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     12212..12231
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             12293..13096
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12356..12375)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12966..12985
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             13240..13473
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    13545..13666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13582..13601)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13636..13655
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      13706..13841
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(13862..13880)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(13890..13907)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13890..13906)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13899..13921)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      14048..14476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(14135..14154)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14345..14366
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca
     4201 tgggaaccga cattgattat tgactaggct tttgcaaaaa gctttgcaaa gatggataaa
     4261 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     4321 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4381 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     4441 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4501 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4561 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4621 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     4681 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     4741 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     4801 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     4861 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     4921 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     4981 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5041 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5101 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5161 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5221 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5281 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5341 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5401 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5461 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact
     5521 aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat
     5581 ccatgtaccc atacgatgtt ccagattacg ctggtggatc tggaggttct ggtggaatgt
     5641 ccgactcgtg ggtcccgaac tccgcctcgg gccaggaccc agggggccgc cggagggcct
     5701 gggccgagct gctggcggga agggtcaaga gggaaaaata taatcctgaa agggcacaga
     5761 aattaaagga atcagctgtg cgcctcctgc gaagccatca ggacctgaat gcccttttgc
     5821 ttgaggtaga aggtccactg tgtaaaaaat tgtctctcag caaagtgatt gactgtgaca
     5881 gttctgaggc ctatgctaat cattctagtt catttatagg ctctgctttg caggatcaag
     5941 cctcaaggct gggggttccc gtgggtattc tctcagccgg gatggttgcc tctagcgtgg
     6001 gacagatctg cacggctcca gcggagacca gtcaccctgt gctgctgact gtggagcaga
     6061 gaaagaagct gtcttccctg ttagagtttg ctcagtattt attggcacac agtatgttct
     6121 cccgtctttc cttctgtcaa gaattatgga aaatacagag ttctttgttg cttgaagcgg
     6181 tgtggcatct tcacgtacaa ggcattgtga gcctgcaaga gctgctggaa agccatcccg
     6241 acatgcatgc tgtgggatcg tggctcttca ggaatctgtg ctgcctttgt gaacagatgg
     6301 aagcatcctg ccagcatgct gacgtcgcca gggccatgct ttctgatttt gttcaaatgt
     6361 ttgttttgag gggatttcag aaaaactcag atctgagaag aactgtggag cctgaaaaaa
     6421 tgccgcaggt cacggttgat gtactgcaga gaatgctgat ttttgcactt gacgctttgg
     6481 ctgctggagt acaggaggag tcctccactc acaagatcgt gaggtgctgg ttcggagtgt
     6541 tcagtggaca cacgcttggc agtgtaattt ccacagatcc tctgaagagg ttcttcagtc
     6601 ataccctgac tcagatactc actcacagcc ctgtgctgaa agcatctgat gctgttcaga
     6661 tgcagagaga gtggagcttt gcgcggacac accctctgct cacctcactg taccgcaggc
     6721 tctttgtgat gctgagtgca gaggagttgg ttggccattt gcaagaagtt ctggaaacgc
     6781 aggaggttca ctggcagaga gtgctctcct ttgtgtctgc cctggttgtc tgctttccag
     6841 aagcgcagca gctgcttgaa gactgggtgg cgcgtttgat ggcccaggca ttcgagagct
     6901 gccagctgga cagcatggtc actgcgttcc tggttgtgcg ccaggcagca ctggagggcc
     6961 cctctgcgtt cctgtcatat gcagactggt tcaaggcctc ctttgggagc acacgaggct
     7021 accatggctg cagcaagaag gccctggtct tcctgtttac gttcttgtca gaactcgtgc
     7081 cttttgagtc tccccggtac ctgcaggtgc acattctcca cccacccctg gttcccggca
     7141 agtaccgctc cctcctcaca gactacatct cattggccaa gacacggctg gccgacctca
     7201 aggtttctat agaaaacatg ggactctacg aggatttgtc atcagctggg gacattactg
     7261 agccccacag ccaagctctt caggatgttg aaaaggccat catggtgttt gagcatacgg
     7321 ggaacatccc agtcaccgtc atggaggcca gcatattcag gaggccttac tacgtgtccc
     7381 acttcctccc cgccctgctc acacctcgag tgctccccaa agtccctgac tcccgtgtgg
     7441 cgtttataga gtctctgaag agagcagata aaatcccccc atctctgtac tccacctact
     7501 gccaggcctg ctctgctgct gaagagaagc cagaagatgc agccctggga gtgagggcag
     7561 aacccaactc tgctgaggag cccctgggac agctcacagc tgcactggga gagctgagag
     7621 cctccatgac agaccccagc cagcgtgatg ttatatcggc acaggtggca gtgatttctg
     7681 aaagactgag ggctgtcctg ggccacaatg aggatgacag cagcgttgag atatcaaaga
     7741 ttcagctcag catcaacacg ccgagactgg agccacggga acacatggct gtggacctcc
     7801 tgctgacgtc tttctgtcag aacctgatgg ctgcctccag tgtcgctccc ccggagaggc
     7861 agggtccctg ggctgccctc ttcgtgagga ccatgtgtgg acgtgtgctc cctgcagtgc
     7921 tcacccggct ctgccagctg ctccgtcacc agggcccgag cctgagtgcc ccacatgtgc
     7981 tggggttggc tgccctggcc gtgcacctgg gtgagtccag gtctgcgctc ccagaggtgg
     8041 atgtgggtcc tcctgcacct ggtgctggcc ttcctgtccc tgcgctcttt gacagcctcc
     8101 tgacctgtag gacgagggat tccttgttct tctgcctgaa attttgtaca gcagcaattt
     8161 cttactctct ctgcaagttt tcttcccagt cacgagatac tttgtgcagc tgcttatctc
     8221 caggccttat taaaaagttt cagttcctca tgttcagatt gttctcagag gcccgacagc
     8281 ctctttctga ggaggacgta gccagccttt cctggagacc cttgcacctt ccttctgcag
     8341 actggcagag agctgccctc tctctctgga cacacagaac cttccgagag gtgttgaaag
     8401 aggaagatgt tcacttaact taccaagact ggttacacct ggagctggaa attcaacctg
     8461 aagctgatgc tctttcagat actgaacggc aggacttcca ccagtgggcg atccatgagc
     8521 actttctccc tgagtcctcg gcttcagggg gctgtgacgg agacctgcag gctgcgtgta
     8581 ccattcttgt caacgcactg atggatttcc accaaagctc aaggagttat gaccactcag
     8641 aaaattctga tttggtcttt ggtggccgca caggaaatga ggatattatt tccagattgc
     8701 aggagatggt agctgacctg gagctgcagc aagacctcat agtgcctctc ggccacaccc
     8761 cttcccagga gcacttcctc tttgagattt tccgcagacg gctccaggct ctgacaagcg
     8821 ggtggagcgt ggctgccagc cttcagagac agagggagct gctaatgtac aaacggatcc
     8881 tcctccgcct gccttcgtct gtcctctgcg gcagcagctt ccaggcagaa cagcccatca
     8941 ctgccagatg cgagcagttc ttccacttgg tcaactctga gatgagaaac ttctgctccc
     9001 acggaggtgc cctgacacag gacatcactg cccacttctt caggggcctc ctgaacgcct
     9061 gtctgcggag cagagacccc tccctgatgg tcgacttcat actggccaag tgccagacga
     9121 aatgcccctt aattttgacc tctgctctgg tgtggtggcc gagcctggag cctgtgctgc
     9181 tctgccggtg gaggagacac tgccagagcc cgctgccccg ggaactgcag aagctacaag
     9241 aaggccggca gtttgccagc gatttcctct cccctgaggc tgcctcccca gcacccaacc
     9301 cggactggct ctcagctgct gcactgcact ttgcgattca acaagtcagg gaagaaaaca
     9361 tcaggaagca gctaaagaag ctggactgcg agagagagga gctattggtt ttccttttct
     9421 tcttctcctt gatgggcctg ctgtcgtcac atctgacctc aaatagcacc acagacctgc
     9481 caaaggcttt ccacgtttgt gcagcaatcc tcgagtgttt agagaagagg aagatatcct
     9541 ggctggcact ctttcagttg acagagagtg acctcaggct ggggcggctc ctcctccgtg
     9601 tggccccgga tcagcacacc aggctgctgc ctttcgcttt ttacagtctt ctctcctact
     9661 tccatgaaga cgcggccatc agggaagagg ccttcctgca tgttgctgtg gacatgtact
     9721 tgaagctggt ccagctcttc gtggctgggg atacaagcac agtttcacct ccagctggca
     9781 ggagcctgga gctcaagggt cagggcaacc ccgtggaact gataacaaaa gctcgtcttt
     9841 ttctgctgca gttaatacct cggtgcccga aaaagagctt ctcacacgtg gcagagctgc
     9901 tggctgatcg tggggactgc gacccagagg tgagcgccgc cctccagagc agacagcagg
     9961 ctgcccctga cgctgacctg tcccaggagc ctcatctctt ctgagtcgac cccctctccc
    10021 tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc
    10081 tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc
    10141 cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt
    10201 ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct
    10261 gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa
    10321 aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt
    10381 tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag
    10441 gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt
    10501 acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt
    10561 ttcctttgaa aaacacgatg ccaccatggg tatgaaccca gccatcagcg tcgctctcct
    10621 gctctcagtc ttgcaggtgt cccgagggca gaaggtgacc agcctgacag cctgcctggt
    10681 gaaccaaaac cttcgcctgg actgccgcca tgagaataac accaaggata actccatcca
    10741 gcatgagttc agcctgaccc gagagaagag gaagcacgtg ctctcaggca cccttgggat
    10801 acccgagcac acgtaccgct cccgcgtcac cctctccaac cagccctata tcaaggtcct
    10861 taccctagcc aacttcacca ccaaggatga gggcgactac ttttgtgagc ttcgcgtctc
    10921 gggcgcgaat cccatgagct ccaataaaag tatcagtgtg tatagagaca agctggtcaa
    10981 gtgtggcggc ataagcctgc tggttcagaa cacatcctgg atgctgctgc tgctgctttc
    11041 cctctccctc ctccaagccc tggacttcat ttctctgtga tctagaccca gctttcttgt
    11101 acaaagtggt tgatatccag cacagtggcg gccgctcgac aatcaacctc tggattacaa
    11161 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    11221 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
    11281 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
    11341 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
    11401 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
    11461 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
    11521 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
    11581 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
    11641 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
    11701 tcggatctcc ctttgggccg cctccccgcc tggacctgca ggattctgca gatatccgga
    11761 ctagtgatct aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
    11821 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
    11881 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
    11941 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
    12001 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
    12061 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
    12121 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
    12181 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
    12241 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagcca atatgggatc
    12301 ggccattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
    12361 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
    12421 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
    12481 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    12541 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    12601 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    12661 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    12721 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    12781 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga
    12841 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    12901 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
    12961 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    13021 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    13081 tgacgagttc ttctgaacgc gtcgcccgcc ccacgacccg cagcgcccga ccgaaaggag
    13141 cgcacgaccc catgcatctc gagggcccgg tacctttaag accaatgact tacaaggcag
    13201 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta gctcactccc
    13261 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    13321 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    13381 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    13441 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
    13501 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
    13561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    13621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
    13681 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    13741 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    13801 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
    13861 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    13921 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    13981 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    14041 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    14101 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    14161 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    14221 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    14281 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    14341 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    14401 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    14461 aaatttaacg cgaattttaa caaaatatta acgcttacaa ttt
//
