LOCUS       sequence-209076-        8868 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression vector of CRISPR/dCas9 fused with SET domain of mouse 
            MLL3 gene, driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8868)
  AUTHORS   Yan J, Chen SA, Local A, Liu T, Qiu Y, Dorighi KM, Preissl S, Rivera
            CM, Wang C, Ye Z, Ge K, Hu M, Wysocka J, Ren B
  TITLE     Histone H3 lysine 4 monomethylation modulates long-range chromatin 
            interactions at enhancers.
  JOURNAL   Cell Res. 2018 Jan 9. pii: cr20181. doi: 10.1038/cr.2018.1.
  PUBMED    29313530
REFERENCE   2  (bases 1 to 8868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2018 Jan 9. pii: cr20181. doi: 10.1038/cr.2018.1."
COMMENT     Sequence Label: CBh-dCas9-Mll3SET
FEATURES             Location/Qualifiers
     source          1..8868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             16..81
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(85..111,112..114,115..2598,2599..2604,2605..2670,
                     2671..2673,2674..4188)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(85..111,112..114,115..2601,2602..2604,2605..4188)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             85..4188
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     protein_bind    4189..4213
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4781..4805)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4819..4839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4892..5514
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5091..5110)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5538..5558)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5543..5872
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5723..5858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5910..5929)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5911..5929)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5943..5959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5943..5959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5956..5978)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5967..5983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5991..5997,5998..6015,6016..6021))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6036..6057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6174..6191)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6345..6933)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6425..6444)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7104..7895,7896..7964))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7727..7746
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7965..8069)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8096..8551
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8183..8202)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8393..8414
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8677..8699
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8691..8708
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8692..8708
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accggtgcca ccatggacta taaggaccac gacggagact acaaggatca tgatatcgat
       61 tacaaggatg acgatgacaa gctcatggac aagaagtact ccattgggct cgctatcggc
      121 acaaacagcg tcggctgggc cgtcattacg gacgagtaca aggtgccgag caaaaaattc
      181 aaagttctgg gcaataccga tcgccacagc ataaagaaga acctcattgg cgccctcctg
      241 ttcgactccg gggagacggc cgaagccacg cggctcaaaa gaacagcacg gcgcagatat
      301 acccgcagaa agaatcggat ctgctacctg caggagatct ttagtaatga gatggctaag
      361 gtggatgact ctttcttcca taggctggag gagtcctttt tggtggagga ggataaaaag
      421 cacgagcgcc acccaatctt tggcaatatc gtggacgagg tggcgtacca tgaaaagtac
      481 ccaaccatat atcatctgag gaagaagctt gtagacagta ctgataaggc tgacttgcgg
      541 ttgatctatc tcgcgctggc gcatatgatc aaatttcggg gacacttcct catcgagggg
      601 gacctgaacc cagacaacag cgatgtcgac aaactcttta tccaactggt tcagacttac
      661 aatcagcttt tcgaagagaa cccgatcaac gcatccggag ttgacgccaa agcaatcctg
      721 agcgctaggc tgtccaaatc ccggcggctc gaaaacctca tcgcacagct ccctggggag
      781 aagaagaacg gcctgtttgg taatcttatc gccctgtcac tcgggctgac ccccaacttt
      841 aaatctaact tcgacctggc cgaagatgcc aagcttcaac tgagcaaaga cacctacgat
      901 gatgatctcg acaatctgct ggcccagatc ggcgaccagt acgcagacct ttttttggcg
      961 gcaaagaacc tgtcagacgc cattctgctg agtgatattc tgcgagtgaa cacggagatc
     1021 accaaagctc cgctgagcgc tagtatgatc aagcgctatg atgagcacca ccaagacttg
     1081 actttgctga aggcccttgt cagacagcaa ctgcctgaga agtacaagga aattttcttc
     1141 gatcagtcta aaaatggcta cgccggatac attgacggcg gagcaagcca ggaggaattt
     1201 tacaaattta ttaagcccat cttggaaaaa atggacggca ccgaggagct gctggtaaag
     1261 cttaacagag aagatctgtt gcgcaaacag cgcactttcg acaatggaag catcccccac
     1321 cagattcacc tgggcgaact gcacgctatc ctcaggcggc aagaggattt ctaccccttt
     1381 ttgaaagata acagggaaaa gattgagaaa atcctcacat ttcggatacc ctactatgta
     1441 ggccccctcg cccggggaaa ttccagattc gcgtggatga ctcgcaaatc agaagagacc
     1501 atcactccct ggaacttcga ggaagtcgtg gataaggggg cctctgccca gtccttcatc
     1561 gaaaggatga ctaactttga taaaaatctg cctaacgaaa aggtgcttcc taaacactct
     1621 ctgctgtacg agtacttcac agtttataac gagctcacca aggtcaaata cgtcacagaa
     1681 gggatgagaa agccagcatt cctgtctgga gagcagaaga aagctatcgt ggacctcctc
     1741 ttcaagacga accggaaagt taccgtgaaa cagctcaaag aagactattt caaaaagatt
     1801 gaatgtttcg actctgttga aatcagcgga gtggaggatc gcttcaacgc atccctggga
     1861 acgtatcacg atctcctgaa aatcattaaa gacaaggact tcctggacaa tgaggagaac
     1921 gaggacattc ttgaggacat tgtcctcacc cttacgttgt ttgaagatag ggagatgatt
     1981 gaagaacgct tgaaaactta cgctcatctc ttcgacgaca aagtcatgaa acagctcaag
     2041 aggcgccgat atacaggatg ggggcggctg tcaagaaaac tgatcaatgg gatccgagac
     2101 aagcagagtg gaaagacaat cctggatttt cttaagtccg atggatttgc caacaggaac
     2161 ttcatgcagt tgatccatga tgactctctc acctttaagg aggacatcca gaaagcacaa
     2221 gtttctggcc agggggacag tcttcacgag cacatcgcta atcttgcagg tagcccagct
     2281 atcaaaaagg gaatactgca gaccgttaag gtcgtggatg aactcgtcaa agtaatggga
     2341 aggcataagc ccgagaatat cgttatcgag atggcccgag agaaccaaac tacccagaag
     2401 ggacagaaga acagtaggga aaggatgaag aggattgaag agggtataaa agaactgggg
     2461 tcccaaatcc ttaaggaaca cccagttgaa aacacccagc ttcagaatga gaagctctac
     2521 ctgtactacc tgcagaacgg cagggacatg tacgtggatc aggaactgga catcaatcgg
     2581 ctctccgact acgacgtgga tgccatcgtg ccccagtctt ttctcaaaga tgattctatt
     2641 gataataaag tgttgacaag atccgataaa aatagaggga agagtgataa cgtcccctca
     2701 gaagaagttg tcaagaaaat gaaaaattat tggcggcagc tgctgaacgc caaactgatc
     2761 acacaacgga agttcgataa tctgactaag gctgaacgag gtggcctgtc tgagttggat
     2821 aaagccggct tcatcaaaag gcagcttgtt gagacacgcc agatcaccaa gcacgtggcc
     2881 caaattctcg attcacgcat gaacaccaag tacgatgaaa atgacaaact gattcgagag
     2941 gtgaaagtta ttactctgaa gtctaagctg gtctcagatt tcagaaagga ctttcagttt
     3001 tataaggtga gagagatcaa caattaccac catgcgcatg atgcctacct gaatgcagtg
     3061 gtaggcactg cacttatcaa aaaatatccc aagcttgaat ctgaatttgt ttacggagac
     3121 tataaagtgt acgatgttag gaaaatgatc gcaaagtctg agcaggaaat aggcaaggcc
     3181 accgctaagt acttctttta cagcaatatt atgaattttt tcaagaccga gattacactg
     3241 gccaatggag agattcggaa gcgaccactt atcgaaacaa acggagaaac aggagaaatc
     3301 gtgtgggaca agggtaggga tttcgcgaca gtccggaagg tcctgtccat gccgcaggtg
     3361 aacatcgtta aaaagaccga agtacagacc ggaggcttct ccaaggaaag tatcctcccg
     3421 aaaaggaaca gcgacaagct gatcgcacgc aaaaaagatt gggaccccaa gaaatacggc
     3481 ggattcgatt ctcctacagt cgcttacagt gtactggttg tggccaaagt ggagaaaggg
     3541 aagtctaaaa aactcaaaag cgtcaaggaa ctgctgggca tcacaatcat ggagcgatca
     3601 agcttcgaaa aaaaccccat cgactttctc gaggcgaaag gatataaaga ggtcaaaaaa
     3661 gacctcatca ttaagcttcc caagtactct ctctttgagc ttgaaaacgg ccggaaacga
     3721 atgctcgcta gtgcgggcga gctgcagaaa ggtaacgagc tggcactgcc ctctaaatac
     3781 gttaatttct tgtatctggc cagccactat gaaaagctca aagggtctcc cgaagataat
     3841 gagcagaagc agctgttcgt ggaacaacac aaacactacc ttgatgagat catcgagcaa
     3901 ataagcgaat tctccaaaag agtgatcctc gccgacgcta acctcgataa ggtgctttct
     3961 gcttacaata agcacaggga taagcccatc agggagcagg cagaaaacat tatccacttg
     4021 tttactctga ccaacttggg cgcgcctgca gccttcaagt acttcgacac caccatagac
     4081 agaaagcggt acacctctac aaaggaggtc ctggacgcca cactgattca tcagtcaatt
     4141 acggggctct atgaaacaag aatcgacctc tctcagctcg gtggagacac aagtttgtac
     4201 aaaaaagcag gcttcatgag gcctcacacc ttgaatagca ccagcacttc aaagtcattt
     4261 cagagcacag tcactggaga gctgaatgca ccctacagta agcagtttgt ccactccaag
     4321 tcatcacagt accggagaat gaagactgaa tggaaatcta atgtgtatct ggcccgatct
     4381 cggatccagg gactgggcct gtatgctgct agagacattg aaaaacacac tatggtcatc
     4441 gagtacattg gaacaattat tcgaaatgag gttgcaaacc ggaaggagaa gctttatgag
     4501 tctcagaacc ggggtgtgta catgttccgc atggacaatg accacgtgat tgacgccaca
     4561 ctcacaggag ggcctgcaag atacatcaac cactcctgtg cccctaactg tgtggctgaa
     4621 gtcgtgacct ttgagagagg acacaagatt atcatcagct ccaaccggag gatacagaaa
     4681 ggagaagagc tctgctatga ttataagttt gactttgaag atgaccagca caagattccg
     4741 tgtcactgtg gagcggtgaa ctgccggaag tggatgaaca acccagcttt cttgtacaaa
     4801 gtggttagca gggctgaccc caagaagaag aggaaggtgt aaaagggttg gatccctacc
     4861 ggatcggtac cagtcgactc tagaggatcc cgggtggcat ccctgtgacc cctccccagt
     4921 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     4981 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     5041 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     5101 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     5161 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     5221 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     5281 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     5341 gtgaaccact gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga
     5401 cgtccagaca cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt
     5461 gcttggcact gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac
     5521 gcggccagct tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     5581 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     5641 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5701 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5821 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcct
     5881 cgaggaactg aaaaaccaga aagttaattc cctatagtga gtcgtattaa attcgtaatc
     5941 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     6001 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     6061 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6121 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6181 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6241 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6301 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6361 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6421 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6481 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6541 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6601 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6661 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6721 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6781 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6841 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6901 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6961 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     7021 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     7081 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7141 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7201 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7261 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7321 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7381 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7441 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7501 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7561 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7621 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7681 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7741 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7801 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7861 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7921 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7981 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     8041 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     8101 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     8161 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     8221 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     8281 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8341 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8401 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8461 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8521 aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt
     8581 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8641 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8701 cggccagtgc caagctgatc tatacattga atcaatattg gcaattagcc atattagtca
     8761 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
     8821 ataatatgta catttatatt ggctcatgtc caatatgacc gccatgtt
//
