LOCUS       sequence-209738-         937 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses human Tau with a mutated cysteine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     Bacterial expression plasmids and lentivirus expression vectors for 
            tau and alpha-synuclein
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET29b Tau Cys
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(29..46)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg
       61 caagcttgtc gacggagctc gaattcggat cctatatctc cttcttagct aattcccgaa
      121 ctgcgaggag cagctggggg cagagggcgg gggccgggtc attattcttt ttttttccac
      181 actctctcat tctctcctct ccacaattat tgaccgcccc aggggcctga tcacaaaccc
      241 tgcttggcca gggaggcaga cacctcgtca gctagcgtgg cgagctgggg cgagtctacc
      301 atgtcgatgc tgccggtgga ggagacattg ctgagatgcc gtggagacgt gtccccagac
      361 accactggcg acttgtacac gatctccgcc ccgtggtctg tcttggcttt ggcgttctcg
      421 cggaaggtca gcttgtgggt ttcaatcttt ttatttcctc cgccagggac gtgggtgata
      481 ttgtccaggg acccaatctt cgactggact ctgtccttga agtcaagctt ctcagatttt
      541 acttccacct ggccacctcc tggtttatga tggatgttgc ctaatgagcc tgccttggag
      601 gtcaccttgc tcaggtcaac tggtttgtag actatttgca cactgccgcc tcccgggacg
      661 tgtttgatat tatcctttga gcccgccttg gactggacgt tgctaagatc cagcttctta
      721 ttaattatct gcaccttccc gcctcccggc tggtgcttca ngttctcagt ggagccgcac
      781 ttggacttga cattcttcan gtctggcatg ggcacggggg ctgtctgcan gcggctcttg
      841 gnggaagacg gcgacttggg tggagtacgg accactgcca ccttcttggg ctcccgggtg
      901 ggtggggttg gaagggacgg ggngcggnnc ggctgcc
//
