LOCUS       sequence-209895-        9453 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Baculovirus transfer vector for expression of huntingtin protein in 
            insect and mammalian cellscells as well as in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Harding RJ, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, 
            Holehouse AS, Ho JC, Fan L, Toledo-Sherman L, Seitova A, Arrowsmith 
            CH
  TITLE     Design and characterization of mutant and wildtype huntingtin 
            proteins produced from a toolkit of scalable eukaryotic expression 
            systems.
  JOURNAL   J Biol Chem. 2019 Apr 26;294(17):6986-7001. doi: 
            10.1074/jbc.RA118.007204. Epub 2019 Mar 6.
  PUBMED    30842263
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA118.007204"; journalName: "J Biol Chem"; date: 
            "2019-04-26- 26"; volume: "294"; issue: "17"; pages: "6986-7001"
COMMENT     Sequence Label: pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengt...
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      8771..8780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcgaccc tggaaaagct gatgaaggcc ttcgagtccc tcaagtcctt ccaacagcag
       61 caacagcaac aacagcagca acagcaacaa cagcagcaac agcaacaaca gcagcaacag
      121 caacaacagc agcaacagca accgccaccg ccgccgccgc cgccgccgcc tcctcagctt
      181 cctcagccgc cgccgcaggc acagccgctg ctgcctcagc cgcagccgcc cccgccgccg
      241 cccccgccgc cacccggccc ggctgtggct gaggagccgc tgcaccgacc aaagaaagaa
      301 ctttcagcta ccaagaaaga ccgtgtgaat cattgtctga caatatgtga aaacatagtg
      361 gcacagtctg tcagaaattc tccagaattt cagaaacttc tgggcatcgc tatggaactt
      421 tttctgctgt gcagtgatga cgcagagtca gatgtcagga tggtggctga cgaatgcctc
      481 aacaaagtta tcaaagcttt gatggattct aatcttccaa ggttacagct cgagctctat
      541 aaggaaatta aaaagaatgg tgcccctcgg agtttgcgtg ctgccctgtg gaggtttgct
      601 gagctggctc acctggttcg gcctcagaaa tgcaggcctt acctggtgaa ccttctgccg
      661 tgcctgactc gaacaagcaa gagacccgaa gaatcagtcc aggagacctt ggctgcagct
      721 gttcccaaaa ttatggcttc ttttggcaat tttgcaaatg acaatgaaat taaggttttg
      781 ttaaaggcct tcatagcgaa cctgaagtca agctccccca ccattcggcg gacagcggct
      841 ggatcagcag tgagcatctg ccagcactca agaaggacac aatatttcta tagttggcta
      901 ctaaatgtgc tcttaggctt actcgttcct gtcgaggatg aacactccac tctgctgatt
      961 cttggcgtgc tgctcaccct gaggtatttg gtgcccttgc tgcagcagca ggtcaaggac
     1021 acaagcctga aaggcagctt cggagtgaca aggaaagaaa tggaagtctc tccttctgca
     1081 gagcagcttg tccaggttta tgaactgacg ttacatcata cacagcacca agaccacaat
     1141 gttgtgaccg gagccctgga gctgttgcag cagctcttca gaacgcctcc acccgagctt
     1201 ctgcaaaccc tgaccgcagt cgggggcatt gggcagctca ccgctgctaa ggaggagtct
     1261 ggtggccgaa gccgtagtgg gagtattgtg gaacttatag ctggaggggg ttcctcatgc
     1321 agccctgtcc tttcaagaaa acaaaaaggc aaagtgctct taggagaaga agaagccttg
     1381 gaggatgact ctgaatcgag atcggatgtc agcagctctg ccttaacagc ctcagtgaag
     1441 gatgagatca gtggagagct ggctgcttct tcaggggttt ccactccagg gtcagcaggt
     1501 catgacatca tcacagaaca gccacggtca cagcacacac tgcaggcgga ctcagtggat
     1561 ctggccagct gtgacttgac aagctctgcc actgatgggg atgaggagga tatcttgagc
     1621 cacagctcca gccaggtcag cgccgtccca tctgaccctg ccatggacct gaatgatggg
     1681 acccaggcct cgtcgcccat cagcgacagc tcccagacca ccaccgaagg gcctgattca
     1741 gctgttaccc cttcagacag ttctgaaatt gtgttagacg gtaccgacaa ccagtatttg
     1801 ggcctgcaga ttggacagcc ccaggatgaa gatgaggaag ccacaggtat tcttcctgat
     1861 gaagcctcgg aggccttcag gaactcttcc atggcccttc aacaggcaca tttattgaaa
     1921 aacatgagtc actgcaggca gccttctgac agcagtgttg ataaatttgt gttgagagat
     1981 gaagctactg aaccgggtga tcaagaaaac aagccttgcc gcatcaaagg tgacattgga
     2041 cagtccactg atgatgactc tgcacctctt gtccattgtg tccgcctttt atctgcttcg
     2101 tttttgctaa cagggggaaa aaatgtgctg gttccggaca gggatgtgag ggtcagcgtg
     2161 aaggccctgg ccctcagctg tgtgggagca gctgtggccc tccacccgga atctttcttc
     2221 agcaaactct ataaagttcc tcttgacacc acggaatacc ctgaggaaca gtatgtctca
     2281 gacatcttga actacatcga tcatggagac ccacaggttc gaggagccac tgccattctc
     2341 tgtgggaccc tcatctgctc catcctcagc aggtcccgct tccacgtggg agattggatg
     2401 ggcaccatta gaaccctcac aggaaataca ttttctttgg cggattgcat tcctttgctg
     2461 cggaaaacac tgaaggatga gtcttctgtt acttgcaagt tagcttgtac agctgtgagg
     2521 aactgtgtca tgagtctctg cagcagcagc tacagtgagt taggactgca gctgatcatc
     2581 gatgtgctga ctctgaggaa cagttcctat tggctggtga ggacagagct tctggaaacc
     2641 cttgcagaga ttgacttcag gctggtgagc tttttggagg caaaagcaga aaacttacac
     2701 agaggggctc atcattatac agggctttta aaactgcaag aacgagtgct caataatgtt
     2761 gtcatccatt tgcttggaga tgaagacccc agggtgcgac atgttgccgc agcatcacta
     2821 attaggcttg tcccaaagct gttttataaa tgtgaccaag gacaagctga tccagtagtg
     2881 gccgtggcaa gagatcaaag cagtgtttac ctgaaacttc tcatgcatga gacgcagcct
     2941 ccatctcatt tctccgtcag cacaataacc agaatatata gaggctataa cctactacca
     3001 agcataacag acgtcactat ggaaaataac ctttcaagag ttattgcagc agtttctcat
     3061 gaactaatca catcaaccac cagagcactc acatttggat gctgtgaagc tttgtgtctt
     3121 ctttccactg ccttcccagt ttgcatttgg agtttaggtt ggcactgtgg agtgcctcca
     3181 ctgagtgcct cagatgagtc taggaagagc tgtaccgttg ggatggccac aatgattctg
     3241 accctgctct cgtcagcttg gttcccattg gatctctcag cccatcaaga tgctttgatt
     3301 ttggccggaa acttgcttgc agccagtgct cccaaatctc tgagaagttc atgggcctct
     3361 gaagaagaag ccaacccagc agccaccaag caagaggagg tctggccagc cctgggggac
     3421 cgggccctgg tgcccatggt ggagcagctc ttctctcacc tgctgaaggt gattaacatt
     3481 tgtgcccacg tcctggatga cgtggctcct ggacccgcaa taaaggcagc cttgccttct
     3541 ctaacaaacc ccccttctct aagtcccatc cgacgaaagg ggaaggagaa agaaccagga
     3601 gaacaagcat ctgtaccgtt gagtcccaag aaaggcagtg aggccagtgc agcttctaga
     3661 caatctgata cctcaggtcc tgttacaaca agtaaatcct catcactggg gagtttctat
     3721 catcttcctt catacctcaa actgcatgat gtcctgaaag ctacacacgc taactacaag
     3781 gtcacgctgg atcttcagaa cagcacggaa aagtttggag ggtttcttcg ctcagccttg
     3841 gatgttcttt ctcagatact agagctggcc acactgcagg acattgggaa gtgtgttgaa
     3901 gagatcctag gatacctgaa atcctgcttt agtcgagaac caatgatggc aactgtttgt
     3961 gttcaacaat tgttgaagac tctctttggc acaaacttgg cctcccagtt tgatggctta
     4021 tcttccaacc ccagcaagtc acaaggccga gcacagcgcc ttggctcctc cagtgtgagg
     4081 ccaggcttgt accactactg cttcatggcc ccgtacaccc acttcaccca ggccctcgct
     4141 gacgccagcc tgaggaacat ggtgcaggcg gagcaggaga acgacacctc gggatggttt
     4201 gatgtcctcc agaaagtgtc tacccagttg aagacaaacc tcacgagtgt cacaaagaac
     4261 cgtgcagata agaatgctat tcataatcac attcgtttgt ttgaacctct tgttataaaa
     4321 gctttaaaac agtacacgac tacaacatgt gtgcagttac agaagcaggt tttagatttg
     4381 ctggcgcagc tggttcagtt acgggttaat tactgtcttc tggattcaga tcaggtgttt
     4441 attggctttg tattgaaaca gtttgaatac attgaagtgg gccagttcag ggaatcagag
     4501 gcaatcattc caaacatctt tttcttcttg gtattactat cttatgaacg ctatcattca
     4561 aaacagatca ttggaattcc taaaatcatt cagctctgtg atggcatcat ggccagtgga
     4621 aggaaggctg tgacacatgc cataccggct ctgcagccca tagtccacga cctctttgta
     4681 ttaagaggaa caaataaagc tgatgcagga aaagagcttg aaacccaaaa agaggtggtg
     4741 gtgtcaatgt tactgagact catccagtac catcaggtgt tggagatgtt cattcttgtc
     4801 ctgcagcagt gccacaagga gaatgaagac aagtggaagc gactgtctcg acagatagct
     4861 gacatcatcc tcccaatgtt agccaaacag cagatgcaca ttgactctca tgaagccctt
     4921 ggagtgttaa atacattatt tgagattttg gccccttcct ccctccgtcc ggtagacatg
     4981 cttttacgga gtatgttcgt cactccaaac acaatggcgt ccgtgagcac tgttcaactg
     5041 tggatatcgg gaattctggc cattttgagg gttctgattt cccagtcaac tgaagatatt
     5101 gttctttctc gtattcagga gctctccttc tctccgtatt taatctcctg tacagtaatt
     5161 aataggttaa gagatgggga cagtacttca acgctagaag aacacagtga agggaaacaa
     5221 ataaagaatt tgccagaaga aacattttca aggtttctat tacaactggt tggtattctt
     5281 ttagaagaca ttgttacaaa acagctgaag gtggaaatga gtgagcagca acatactttc
     5341 tattgccagg aactaggcac actgctaatg tgtctgatcc acatcttcaa gtctggaatg
     5401 ttccggagaa tcacagcagc tgccactagg ctgttccgca gtgatggctg tggcggcagt
     5461 ttctacaccc tggacagctt gaacttgcgg gctcgttcca tgatcaccac ccacccggcc
     5521 ctggtgctgc tctggtgtca gatactgctg cttgtcaacc acaccgacta ccgctggtgg
     5581 gcagaagtgc agcagacccc gaaaagacac agtctgtcca gcacaaagtt acttagtccc
     5641 cagatgtctg gagaagagga ggattctgac ttggcagcca aacttggaat gtgcaataga
     5701 gaaatagtac gaagaggggc tctcattctc ttctgtgatt atgtctgtca gaacctccat
     5761 gactccgagc acttaacgtg gctcattgta aatcacattc aagatctgat cagcctttcc
     5821 cacgagcctc cagtacagga cttcatcagt gccgttcatc ggaactctgc tgccagcggc
     5881 ctgttcatcc aggcaattca gtctcgttgt gaaaaccttt caactccaac catgctgaag
     5941 aaaactcttc agtgcttgga ggggatccat ctcagccagt cgggagctgt gctcacgctg
     6001 tatgtggaca ggcttctgtg cacccctttc cgtgtgctgg ctcgcatggt cgacatcctt
     6061 gcttgtcgcc gggtagaaat gcttctggct gcaaatttac agagcagcat ggcccagttg
     6121 ccaatggaag aactcaacag aatccaggaa taccttcaga gcagcgggct cgctcagaga
     6181 caccaaaggc tctattccct gctggacagg tttcgtctct ccaccatgca agactcactt
     6241 agtccctctc ctccagtctc ttcccacccg ctggacgggg atgggcacgt gtcactggaa
     6301 acagtgagtc cggacaaaga ctggtacgtt catcttgtca aatcccagtg ttggaccagg
     6361 tcagattctg cactgctgga aggtgcagag ctggtgaatc ggattcctgc tgaagatatg
     6421 aatgccttca tgatgaactc ggagttcaac ctaagcctgc tagctccatg cttaagccta
     6481 gggatgagtg aaatttctgg tggccagaag agtgcccttt ttgaagcagc ccgtgaggtg
     6541 actctggccc gtgtgagcgg caccgtgcag cagctccctg ctgtccatca tgtcttccag
     6601 cccgagctgc ctgcagaacc ggcggcctac tggagcaagt tgaatgatct gtttggggat
     6661 gctgcactgt atcagtccct gcccactctg gcccgggccc tggcacagta cctggtggtg
     6721 gtctccaaac tgcccagtca tttgcacctt cctcctgaga aagagaagga cattgtgaaa
     6781 ttcgtggtgg caacccttga ggccctgtcc tggcatttga tccatgagca gatcccgctg
     6841 agtctggatc tccaggcagg gctggactgc tgctgcctgg ccctgcagct gcctggcctc
     6901 tggagcgtgg tctcctccac agagtttgtg acccacgcct gctccctcat ccactgtgtg
     6961 cacttcatcc tggaggccgt tgcagtgcag cctggagagc agcttcttag tccagaaaga
     7021 aggacaaata ccccaaaagc catcagcgag gaggaggagg aagtagatcc aaacacacag
     7081 aatcctaagt atatcactgc agcctgtgag atggtggcag aaatggtgga gtctctgcag
     7141 tcggtgttgg ccttgggtca taaaaggaat agcggcgtgc cggcgtttct cacgccattg
     7201 ctcaggaaca tcatcatcag cctggcccgc ctgccccttg tcaacagcta cacacgtgtg
     7261 cccccactgg tgtggaagct tggatggtca cccaaaccgg gaggggattt tggcacagca
     7321 ttccctgaga tccccgtgga gttcctccag gaaaaggaag tctttaagga gttcatctac
     7381 cgcatcaaca cactaggctg gaccagtcgt actcagtttg aagaaacttg ggccaccctc
     7441 cttggtgtcc tggtgacgca gcccctcgtg atggagcagg aggagagccc accagaagaa
     7501 gacacagaga ggacccagat caacgtcctg gccgtgcagg ccatcacctc actggtgctc
     7561 agtgcaatga ctgtgcctgt ggccggcaac ccagctgtaa gctgcttgga gcagcagccc
     7621 cggaacaagc ctctgaaagc tctcgacacc aggtttggga ggaagctgag cattatcaga
     7681 gggattgtgg agcaagagat tcaagcaatg gtttcaaaga gagagaatat tgccacccat
     7741 catttatatc aggcatggga tcctgtccct tctctgtctc cggctactac aggtgccctc
     7801 atcagccacg agaagctgct gctgcagatc aaccccgagc gggagctggg gagcatgagc
     7861 tacaaactcg gccaggtgtc catacactcc gtgtggctag ggaacagcat cacacccctg
     7921 agggaggagg aatgggacga ggaagaggag gaggaggccg acgcccctgc accttcgtca
     7981 ccacccacgt ctccagtcaa ctccaggaaa caccgggctg gagttgacat ccactcctgt
     8041 tcgcagtttt tgcttgagtt gtacagccgc tggatcctgc cgtccagctc agccaggagg
     8101 accccggcca tcctgatcag tgaggtggtc agatcccttc tagtggtctc agacttgttc
     8161 accgagcgca accagtttga gctgatgtat gtgacgctga cagaactgcg aagggtgcac
     8221 ccttcagaag acgagatcct cgctcagtac ctggtgcctg ccacctgcaa ggcagctgcc
     8281 gtccttggga tggacaaggc cgtggcggag cctgtcagcc gcctgctgga gagcacgctc
     8341 aggagcagcc acctgcccag cagggttgga gccctgcacg gcatcctcta tgtgctggag
     8401 tgcgacctgc tggacgacac tgccaagcag ctcatcccgg tcatcagcga ctatctcctc
     8461 tccaacctga aagggatcgc ccactgcgtg aacattcaca gccagcagca cgtactggtc
     8521 atgtgtgcca ctgcgtttta cctcattgag aactatcctc tggacgtagg gccggaattt
     8581 tcagcatcaa taatacagat gtgtggggtg atgctgtctg gaagtgagga gtccaccccc
     8641 tccatcattt accactgtgc cctcagaggc ctggagcgcc tcctgctctc tgagcagctc
     8701 tcccgcctgg atgcagaatc gctggtcaag ctgagtgtgg acagagtgaa cgtgcacagc
     8761 ccgcaccggg ccatggcggc tctgggcctg atgctcacct gcatgtacac aggaaaggag
     8821 aaagtcagtc cgggtagaac ttcagaccct aatcctgcag cccccgacag cgagtcagtg
     8881 attgttgcta tggagcgggt atctgttctt tttgatagga tcaggaaagg ctttccttgt
     8941 gaagccagag tggtggccag gatcctgccc cagtttctag acgacttctt cccaccccag
     9001 gacatcatga acaaagtcat cggagagttt ctgtccaacc agcagccata cccccagttc
     9061 atggccaccg tggtgtataa ggtgtttcag actctgcaca gcaccgggca gtcgtccatg
     9121 gtccgggact gggtcatgct gtccctctcc aacttcacgc agagggcccc ggtcgccatg
     9181 gccacgtgga gcctctcctg cttctttgtc agcgcgtcca ccagcccgtg ggtcgcggcg
     9241 atcctcccac atgtcatcag caggatgggc aagctggagc aggtggacgt gaaccttttc
     9301 tgcctggtcg ccacagactt ctacagacac cagatagagg aggagctcga ccgcagggcc
     9361 ttccagtctg tgcttgaggt ggttgcagcc ccaggaagcc catatcaccg gctgctgact
     9421 tgtttacgaa atgtccacaa ggtcaccacc tgc
//
