LOCUS       sequence-209924-        9587 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Low level expression of mCherry-EGFP-PIM. Can be used for inducible 
            aggregate formation upon AP20187 addition.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Janssen AFJ, Katrukha EA, van Straaten W, Verlhac P, Reggiori F, 
            Kapitein LC
  TITLE     Probing aggrephagy using chemically-induced protein aggregates.
  JOURNAL   Nat Commun. 2018 Oct 12;9(1):4245. doi: 10.1038/s41467-018-06674-4.
  PUBMED    30315152
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-10-12"; volume: "9(1):4245. doi"; pages: " 
            10.1038/s41467-018-06674-4"
COMMENT     Sequence Label: pBA-mCherry-EGFP-PIM
FEATURES             Location/Qualifiers
     source          1..9587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     928..950
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1029..1349
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1356..1676
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="GVQVETISPGDGRTFPKRGQTCVEHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDCAYGATGHPGIIPPHTTLVFDVELL
                     KLE"
     CDS             1692..2396
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(1834..1852)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2102..2121
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(2406..2408,2409..3119)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(2406..2408,2409..3119)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2448..2469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2709..2728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3056..3077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3810..4130
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4137..4457
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /note="synthetic gene with alternative codons"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             4137..4457
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     polyA_signal    4743..4877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4780..4799)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4834..4853
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4892..4908)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4892..4908)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4905..4927)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4916..4932
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4940..4946,4947..4964,4965..4970))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4985..5006
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5123..5140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5294..5882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5374..5393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6053..6844,6845..6913))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6676..6695
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6914..7018)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7086..7104
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7142..7164)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7264..7283
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7477..7499
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7491..7508
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7492..7508
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9307..9582
                     /label=chicken beta-actin promoter
ORIGIN
        1 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
       61 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
      121 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
      181 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
      241 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
      301 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
      361 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
      421 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
      481 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg
      541 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
      601 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag
      661 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
      721 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      781 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
      841 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct
      901 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggggttt
      961 atatcttccc ttctctgttc ctccgcagcc cccaagctag cgctaagctt gccaccatgc
     1021 gctctagagg cgtccaagtc gaaaccatta gtcccggcga tggcagaaca tttcctaaaa
     1081 ggggacaaac atgtgtcgtc cattatacag gcatgttgga ggacggcaaa aaggtggaca
     1141 gtagtagaga tcgcaataaa cctttcaaat tcatgttggg aaaacaagaa gtcattaggg
     1201 gatgggagga gggcgtggct caaatgtccg tcggccaacg cgctaagctc accatcagcc
     1261 ccgactacgc atacggcgct accggacatc ccggaattat tccccctcac gctaccttgg
     1321 tgtttgacgt cgaactgttg aagctcgaga ctagaggagt gcaggtggag actatctccc
     1381 caggagacgg gcgcaccttc cccaagcgcg gccagacctg cgtggagcac tacaccggga
     1441 tgcttgaaga tggaaagaaa gttgattcct cccgggacag aaacaagccc tttaagttta
     1501 tgctaggcaa gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg
     1561 gtcagagagc caaactgact atatctccag attgtgccta tggtgccact gggcacccag
     1621 gcatcatccc accacatacc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta
     1681 gtgaaccggt agtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     1741 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1801 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1861 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1921 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1981 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     2041 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2101 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2161 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2221 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2281 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2341 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggcg
     2401 cgcctgtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2461 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2521 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     2581 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2641 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2701 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2761 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2821 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2881 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2941 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     3001 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     3061 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     3121 ctagccgctc acgcggcgtt caggtggaga ctataagccc gggggatggt cgcacttttc
     3181 ctaaacgagg ccagacgtgc gtagttcact ataccggcat gcttgaagac ggcaagaagg
     3241 tggatagctc tagggaccgg aataagccct tcaagttcat gcttggtaag caagaagtga
     3301 tccggggttg ggaagagggg gttgcccaaa tgtcagtcgg acaacgggcg aagttgacta
     3361 tttctcctga ttacgcttat ggtgcgacgg gacatcctgg catcataccg cctcacgcca
     3421 ctcttgtctt tgatgtggaa cttttgaagc tggaaacgcg aggggttcaa gttgagacca
     3481 tttcaccggg cgatggcaga acattcccga aaaggggaca aacgtgtgta gtacactata
     3541 cgggcatgct cgaagacggg aaaaaggttg actcaagtag agatcggaac aagcctttca
     3601 agttcatgtt ggggaaacaa gaggtaatac gcgggtggga ggagggagtc gctcagatgt
     3661 ctgtaggcca aagggcgaag ctcaccatat caccagatta cgcttacggg gcgacgggcc
     3721 atcccggaat aatacctccg cacgcgacac ttgtttttga tgttgaattg cttaagttgg
     3781 agaccagcag atctgtcgac cgctctagag gagtgcaggt ggaaaccatc tccccaggag
     3841 acgggcgcac cttccccaag cgcggccaga cctgcgtggt gcactacacc gggatgcttg
     3901 aagatggaaa gaaatttgac tcctcccggg acagaaacaa gccctttaag tttatgctag
     3961 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
     4021 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
     4081 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaactggaa actagaggcg
     4141 tgcaggtcga gaccatcagc cccggcgacg gccgcacctt tcccaagaga ggccagactt
     4201 gcgtggtcca ctacaccggc atgctggagg acggcaagaa gttcgacagc agccgcgacc
     4261 gcaacaagcc cttcaagttc atgctgggca aacaggaagt gatccgcggc tgggaggaag
     4321 gcgtggctca gatgagcgtg gggcagcggg ccaagctgac catcagcccc gactatgcct
     4381 acggcgccac cggccacccc ggcatcatcc ccccccacgc caccctggtg ttcgacgtgg
     4441 agctgctgaa gctggagact agttaggaat tcaggatcca gtcgactagt acttctcgag
     4501 ctctgtacat gtccgcggtc gcgacgtacg cgtatcgatg gcgccagctg caggcggccg
     4561 ccatatgcat cctaggccta ttaatattcc ggagtatacg tagccggcta acaaccggta
     4621 cctctagata agtaatgatc ataatcagcc atatcacatc tgtagaggtt ttacttgctt
     4681 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     4741 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4861 cttatcatgt ctggatcaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     4921 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     4981 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5041 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5101 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5161 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5221 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5281 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5341 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5401 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5461 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5521 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5581 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5641 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5701 tgaagtggtg gcctaactac ggctacagta gaaggacagt atttggtatc tgcgctctgc
     5761 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5821 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5881 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5941 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6001 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6061 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6361 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6481 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6661 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6901 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7021 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7081 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     7141 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     7201 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggt tggcttaact
     7261 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7321 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     7381 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7441 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7501 cggccagtgc caagttggga tctttgcatt ggcccacggc tctcaggatg gggatgctcc
     7561 ccttcagcac ccggttcccc ttggaaactg atggtcctgg ctctgtggca tggcagtggc
     7621 actgtgagga gcccctacca gcagcacaca gtgggtttgg cactgccacg ctccggatgc
     7681 cgcgctctga tccaacccca taatcaaggg aacccgaatt gccccatcat tgcccccacc
     7741 acccccatcc tgccgggccc tcacacccca cgctgccttg tggtgacatt ccccagccca
     7801 aacccacggc ttcatggcta ccgcggggca tttcccattg ccgccccatt atcagctctg
     7861 cacacctccc gctgtaccca tgcctcgtgg ctgcccttct ttgacgtata atcttctaat
     7921 taatacccgg ccttgtcaaa gtggagcaca aacgttaatt aattccccag caggcaggta
     7981 attaacagtg tgactccctt tttgctgcga gtggggctga tacagagaga tgtggcacta
     8041 tggagcccac ggggtcctgg cactgggtgc ccacggaggt ccccatgtgc tgcagtgtca
     8101 ccgcctccga ggtgacagta ttgtccctgc ggtgtccctg cagctcagct ctgtccacag
     8161 ggccacctcc agtttggagg ggacacaatg cagccccgat gcaacccatc ctcgcagcat
     8221 cccagggaca aagaccccac tgcaagaccg cacacagggc tgggtcccgc tcccctaata
     8281 tctacagtgc ttttgcatgg ccccttaatc aatgcagtta atcagcatgc gctcatgcac
     8341 cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca acaccgcgca ccgccccggc
     8401 ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa tattgcccaa atgtaggcaa
     8461 aggcattcgg ctgccttgac ctccgccggg ccgggccctg cctgactcag ctccttactc
     8521 agcgctcgct tcctccctcc ggctgccacc gccgcagcgc acaccctgac aaagagtggc
     8581 ccttaacggg ctctgaggtg cacccagcag tgcactcagc agtccaaggg ccggcctgga
     8641 ggtttgcacc gctacgtgct gacattagca ttgaacttgg ccctgggtag tgctgcaggc
     8701 cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc cggtgcccct tcccctccct
     8761 tgcatcccag caggctgcac cccagcacca ggcccgtgca tgcatgctcc tggtgttatt
     8821 gcagcctggt gcatgcatgc gtcttagtgg tgcagcgctg tgcatgcatc ctccttggtg
     8881 tgtagcagct tagtgcatgc atacccctcg gtgttattgc tgctctgtgc aggcacgctc
     8941 attgtatcac ttcatcccag tgcatgcact cacactggag cgattgctgc tcggtgcacg
     9001 cacactcatt gtatcacgtc agctcagtgg ctgcacgcac accggtgtta ttgctgctcg
     9061 gtgcgtgcat gcacatcagt gtcgctgcag ctcagtgcat gcacgctcat tgcccatcgc
     9121 tatccctgcc tctcctgctg gcgctccccg ggaggtgact tcaaggggac cgcaggacca
     9181 cctcgggggt ggggggaggg ctgcacacgc ggaccccgct ccccctcccc aacaaagcac
     9241 tgtggaatca aaaagggggg aggggggatg gaggggcgcg tcacaccccc gccccacacc
     9301 ctcacctcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     9361 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     9421 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
     9481 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
     9541 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagt
//
