LOCUS       sequence-209928-        2907 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Plasmid for the expression of the construct SNCA-5Asp. 
            Alpha-Synuclein with 22 aa C-terminal moiety, which consists of 9 
            Asn and 13 Gly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Afitska K, Fucikova A, Shvadchak VV, Yushchenko DA
  TITLE     Modification of C Terminus Provides New Insights into the Mechanism 
            of alpha-Synuclein Aggregation.
  JOURNAL   Biophys J. 2017 Nov 21;113(10):2182-2191. doi: 
            10.1016/j.bpj.2017.08.027. Epub 2017 Sep 20.
  PUBMED    28939194
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2017.08.027"; journalName: "Biophys J"; date: 
            "2017-11-21- 21"; volume: "113"; issue: "10"; pages: "2182-2191"
COMMENT     Sequence Label: r-22. pT7-7-SNCA-9Asn
FEATURES             Location/Qualifiers
     source          1..2907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     637..659
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     724..743
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        724..742
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             774..796
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(join(1289..1294,1295..1311,1312..1317))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             complement(join(1370..2161,2162..2230))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1993..2012
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2231..2335)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2403..2421
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      join(2550..2907,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
      421 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatagg aagatcttcc
      481 ggaagatctt cctatggtgc actctcagta caatctgctc tgatgcgcta cgtgactggg
      541 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      601 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      661 tttcaccgtc atcaccgaaa cgcgcgaggc ccagcgattc gaacttctga tagacttcga
      721 aattaatacg actcactata gggagaccac aacggtttcc ctctagaaat aattttgttt
      781 aactttaaga aggagatata catatggatg tattcatgaa aggactttca aaggccaagg
      841 agggagttgt ggctgctgct gagaaaacca aacagggtgt ggcagaagca gcaggaaaga
      901 caaaagaggg tgttctctat gtaggctcca aaaccaagga gggagtggtg catggtgtgg
      961 caacagtggc tgagaagacc aaagagcaag tgacaaatgt tggaggagca gtggtgacgg
     1021 gtgtgacagc agtagcccag aagacagtgg agggagcagg gagcattgca gcagccactg
     1081 gctttgtcaa aaaggaccag ttgggcaaga atgaagaagg agccccacag gaaggaattc
     1141 tggaagatat gcctgtggat cctgacaatg aggcttatga aatgccttct gaggaagggt
     1201 atcaagacta tgaacctgaa gccggcggca acggcaacgg caacggcaac ggcggaggaa
     1261 atggaaatgg aaatggaaat ggaaatggat aaaagcttat cgatgataag ctgtcaaaca
     1321 tgagaattaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     1381 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1441 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1501 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1561 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1621 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1681 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1741 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1801 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1861 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1921 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1981 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2041 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2101 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2161 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2221 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     2281 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     2341 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     2401 aaaataggcg tatcacgagg ccctttcgtc ttcaagaata aaaggatcta ggtgaagatc
     2461 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     2521 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2581 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2641 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2701 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2761 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2821 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2881 tgcacacagc ccagcttgga gcgaacg
//
