LOCUS       sequence-210000-         930 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian expression or 3rd generation lentivirus generation of 
            hSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Kim H, An S, Ro SH, Teixeira F, Park GJ, Kim C, Cho CS, Kim JS, 
            Jakob U, Lee JH, Cho US
  TITLE     Janus-faced Sestrin2 controls ROS and mTOR signalling through two 
            separate functional domains.
  JOURNAL   Nat Commun. 2015 Nov 27;6:10025. doi: 10.1038/ncomms10025.
  PUBMED    26612684
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-27"; volume: "6:10025. doi"; pages: " 
            10.1038/ncomms10025"
COMMENT     Sequence Label: pLU-CMV-Flag-hSesn2 R264P
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      96..105
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             105..128
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tggactntta gcagagcttg nttngtgaac cgntcagaat cgcctggaga cgccatccac
       61 gcctgttttg acctccatag aagacaccga ctctagccac catggactac aaagacgatg
      121 acgacaagtc tagaatgatc gtggcggact ccgagtgccg cgcagagctc aaggactacc
      181 tgcggttcgc cccgggcggc gtcggcgact cgggccccgg agaggagcag agggagagcc
      241 gggctcggcg aggccctcga gggcccagcg ccttcatccc cgtggaggag gtccttcggg
      301 agggggctga gagcctcgag cagcacctgg ggctggaggc actgatgtcc tctgggcgag
      361 tagacaacct ggcagtggtg atgggcctgc accctgacta ctttaccagc ttctggcgcc
      421 tgcactacct gctgctgcac acggatggtc ccttggccag ctcctggcgc cactacattg
      481 ccatcatggc tgccgcccgc catcagtgtt cttacctggt aggctcccac atggccgagt
      541 ttctgcagac tggtggtgac cctgagtggc tgctgggcct ccaccgggcc cccgagaagc
      601 tgcgcaaact cagcgagatc aacaagttgc tggcgcatcg gccatggctc atcaccaagg
      661 aacacatcca ggccttgctg aagaccggcg agcacacttg gtccctggcc gagctcattc
      721 aggctctggt cctgctcacc cactgccact cgctctcctc cttcgtgttt ggctgtggca
      781 tcctccctga gggggatgca gatggcagcc ctgcccccca ggcacctaca ccccctagtg
      841 aacagagcag ccccccaagc agggacccgt tgaacaactc tgggggcttt gagtctgccc
      901 gcgacgtgga agcgctgatg nagcccatgc
//
