LOCUS       sequence-210026-         931 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Mammalian Expression of mSesn2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Ro SH, Semple IA, Park H, Park H, Park HW, Kim M, Kim JS, Lee JH
  TITLE     Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of 
            p62/sequestosome-1.
  JOURNAL   FEBS J. 2014 Sep;281(17):3816-27. doi: 10.1111/febs.12905. Epub 2014
            Jul 28.
  PUBMED    25040165
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/febs.12905"; journalName: "FEBS J"; date: "2014-09"; 
            volume: "281"; issue: "17"; pages: "3816-27"
COMMENT     Sequence Label: pcDNA-Flag-Sesn-B+C mSesn2 (delA)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     58..77
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        58..76
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             117..140
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(870..888)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(871..888)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(908..925)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggnnttagng agctctctgg ctactagaga acccactgct tactggctta tcgaaattaa
       61 tacgactcac tatagggaga cccaagcttg gtaccgagct cggatccacc gccatggact
      121 acaaggacga tgatgacaag ggcgaattcg gctttgaagc cgctcgtgac gtagaagctc
      181 tgatggaacg catgaggcag cttcaggaga gcctgctgcg ggatgagggg gcttcgcagg
      241 aggaaatgga gaaccgtttt gagctggaga agtcagaaag cctgctggtg accccctcag
      301 cggatatcct ggagccttct ccacacccag acatcctatg ctttgtggag gaccccgcat
      361 ttggatatga agacttcact cgtcgaggga ctcaggcgcc cccaaccttc cgtgcccagg
      421 attatacctg ggaagaccat ggctactcgc tgatccagag gctctaccct gagggagggc
      481 agctgctgga tgagaagttc caggtagcct gcagcctcac ctataacacc atcgccatgc
      541 acagtggcgt ggacacctcc atgctccgca gggccatctg gaactacatc cactgcgtct
      601 ttggcatcag atacgatgac tatgattacg gcgaggtaaa ccagctcctg gaacggaacc
      661 tcaaaatcta tatcaagacg gtggcctgct accctgagaa gacgacccgt aggatgtaca
      721 acctcttttg gaggcacttc cgccactcag agaaggttca tgtgaacttg ctgctccttg
      781 aagcccgcat gcaagcggcc ctgctctatg ccctgcgcgc catcacccgg tacatgacct
      841 gagtcgagca tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc
      901 tgatcagcct cgactgtgcc ttctagttgc c
//
