LOCUS       sequence-210338-        7074 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Xylose-responsive Gal3p-Syn4.1 under control of native GAL3p 
            (promoter)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Endalur Gopinarayanan V, Nair NU
  TITLE     A semi-synthetic regulon enables rapid growth of yeast on xylose.
  JOURNAL   Nat Commun. 2018 Mar 26;9(1):1233. doi: 10.1038/s41467-018-03645-7.
  PUBMED    29581426
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-26"; volume: "9(1):1233. doi"; pages: " 
            10.1038/s41467-018-03645-7"
COMMENT     Sequence Label: pVEG16*
FEATURES             Location/Qualifiers
     source          1..7074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        187..467
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             468..1142
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1241..1696)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1378..1399)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1590..1609
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1826..1848
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1840..1857
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1841..1857
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1867..1886
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1867..1885
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1910..1926
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1911..1927
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4300..4320)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4300..4318)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4339..4355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4339..4355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4352..4374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4363..4379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4387..4393,4394..4411,4412..4417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(4570..4587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4741..5329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4821..4840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5500..6291,6292..6360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6123..6142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6361..6465)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    6502..7005
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     7047..7065
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat
      241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa
      301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa
      361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat
      421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta
      481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg
      541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa
      601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa
      661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg
      721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt
      781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag
      841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg
      901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg
      961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa
     1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg
     1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat
     1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga
     1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt
     1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     1861 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
     1921 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccgcata aacaccatca
     1981 gcctcaagtc gtcaagtaaa gatttcgtgt tcatgcagat agataacaat ctatatgttg
     2041 ataattagcg ttgcctcatc aatgcgagat ccgtttaacc ggaccctagt gcacttaccc
     2101 cacgttcggt ccactgtgtg ccgaacatgc tccttcacta ttttaacatg tggaattctt
     2161 gaaagaatga aatcgccatg ccaagccatc acacggtctt ttatgcaatt gattgaccgc
     2221 ctgcaacaca taggcagtaa aatttttact gaaacgtata taatcatcat aagcgacaag
     2281 tgaggcaaca cctttgttac cacattgaca accccaggta ttcatacttc ctattagcgg
     2341 aatcaggagt gcaaaaagag aaaataaaag taaaaaggta gggcaacaca tagtatgaat
     2401 acaaacgttc caatattcag ttctccggtc agagatttac caaggtcttt cgaacaaaaa
     2461 catttagcgg ttgtagatgc atttttccaa acataccatg tcaaacctga ttttatcgct
     2521 aggtctcctg gcagagtaaa tctgattggt gagcatatag attattgcga tttttcagtt
     2581 ttgccattag ccattaatat ggatatgctt tgcgcagtta aaattttaga cgaaaaaaat
     2641 ccatccatta ccttaacaaa tgcggaccct aaatttgctc agcgaaagtt tgatctgcct
     2701 ttagatggtt cctacatggt gatagatccg tctgtgtcgg aatggtcgaa ttactttaaa
     2761 tgcggactac atgtggcaca ttcatacttg aaaaaaattg ctccggaaag atttaataat
     2821 acacccttag taggtgcgca gatcttttgc cagagcgata ttcctactgg tggtggactc
     2881 tcatctgcat ttacttgcgc ggcagcacta gccacaatta gagccaatat gggaaaaaat
     2941 tttgatattt ccaaaaaaga cttgacccgc atcacagcgg ttgctgagca ctatgttgga
     3001 gtcaataatg gtggtatgga tcaagcaacg tctgtttatg gggaagaaga tcatgctcta
     3061 tacgtagagt ttaggccaaa actaaaggcc acacctttca agtttcctca attgaaaaat
     3121 catgaaatca gtttcgtcat cgccaatact cttgtaaagt ctaataaatt cgaaactgct
     3181 cctacaaatt acaatttaag agtattagag gtaacagttg ctgccaacgc cttggcgacc
     3241 agatacagcg tggccttacc atctcacaag gacaattcta actcagaaag agggaatcta
     3301 agagatttta tggatgctta ctacgccaga tacgaaaacc aagcccaacc atggaatgga
     3361 gatatcggaa ctggtattga acgtttactc aagatgctac aattggtaga agaatctttc
     3421 tcgaggaaaa agagcggatt cactgtacat gaagcctcta cggcgctaaa ctgttcacgt
     3481 gaggagttca ctagagatta cctgacaact tttcccgtcc gcttccaagt cttgaaacta
     3541 tatcaaagag ctaaacacgt ttactccgaa tccttaaggg tgcttaaggc tttaaaaatg
     3601 atgacaagtg ccacttttca cacggacgaa gatttcttta cagattttgg ccgactaatg
     3661 aatgagtccc aggcctcttg tgataaactt tatgaatgtt cgtgcataga aaccaatcaa
     3721 atatgctcga ttgccctagc aaatggttct ttcggctccc gtctcactgg cgctggttgg
     3781 ggcggttgca ctatccacct tgttcctagt ggcgctaatg ggaacgtgga acaggtacga
     3841 aaagcactaa tcgaaaaatt ctacaatgta agatatccgg atctcacaga tgaagaacta
     3901 aaagacgcaa ttatagtttc gaagcctgcc ttgggtactt gtttgtacga acaataacac
     3961 taaacacctt cttggaacaa cctttaaata ttaaaagttt actcatacct cttacactct
     4021 ataaagaaac aagttggtga caataaaaag attcttgttt tcaagaactt gtcatttgta
     4081 tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat ttatattttt
     4141 tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt aatatcatgc
     4201 gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta acgccgccat
     4261 ccagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
     4321 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     4381 tccacacaac ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     4441 gtaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     4561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     4621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     4681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     5041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     5101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     5161 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     5221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     6001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     6061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     6121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     6181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6481 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
     6541 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
     6601 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
     6661 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     6721 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     6781 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     6841 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     6901 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     6961 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtcc taagaaacca
     7021 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
