LOCUS       sequence-210535-       11327 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression of IKZF4-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11327)
  AUTHORS   Lu G, Middleton RE, Sun H, Naniong M, Ott CJ, Mitsiades CS, Wong KK,
            Bradner JE, Kaelin WG Jr
  TITLE     The myeloma drug lenalidomide promotes the cereblon-dependent 
            destruction of Ikaros proteins.
  JOURNAL   Science. 2014 Jan 17;343(6168):305-9. doi: 10.1126/science.1244917. 
            Epub 2013 Nov 29.
  PUBMED    24292623
REFERENCE   2  (bases 1 to 11327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1244917"; journalName: "Science"; date: 
            "2014-01-17- 17"; volume: "343"; issue: "6168"; pages: "305-9"
COMMENT     Sequence Label: plenti-UBC- IKZF4-3xHA-pGK-PUR
FEATURES             Location/Qualifiers
     source          1..11327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    39..156
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        248..1459
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1296..1315
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1507..1531
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3287..3311)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3358..3447
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        3489..3988
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3524..3547)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3921..3940
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4009..4608
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4009..4028)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4505..4525
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(4639..4672)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    4728..5316
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4781..4801)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5199..5210)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5319..5335)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5320..5336)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5525..5758
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5830..5951
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5867..5886)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5921..5940
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5991..6126
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6146..6165)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6147..6165)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(6188..6546)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6228..6249)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6440..6459
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6573..6677
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6679..7036
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6679..6870
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6702..6722)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6887..7022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7071..7865
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7071..7865
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7125..7144)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7735..7754
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8053..8072)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8097..8144
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8473..9061
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8962..8981
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        9236..9539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9541..9739
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9694..9714
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9757..9937
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9984..10109
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10606..10839
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11024..11068
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
       61 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
      121 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
      181 gatccagttt ggttagtacc gggcccgctc tagccctaga tttaacccaa cagaaggctc
      241 gacgcgtggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
      301 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
      361 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
      421 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
      481 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
      541 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
      601 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
      661 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
      721 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
      781 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
      841 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
      901 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
      961 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
     1021 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
     1081 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
     1141 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     1201 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     1261 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     1321 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     1381 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
     1441 tttggctttt ttgttagacg ctagactgcc ggatcgaatt gtcgacgaat tcggatctgg
     1501 ccaatcacaa gtttgtacaa aaaagttggc atgcatacac cacccgcact ccctcgccgt
     1561 ttccaaggcg gcggccgcgt tcgcacccca gggtctcacc ggcaagggaa ggataatctg
     1621 gagagggatc cctcaggagg gtgtgttccg gatttcttgc ctcaggccca agactccaac
     1681 cattttataa tggaatcttt attttgtgaa agtagcgggg actcatctct ggagaaggag
     1741 ttcctcgggg ccccagtggg gccctcggtg agcaccccca acagccagca ctcttctcct
     1801 agccgctcac tcagtgccaa ctccatcaag gtggagatgt acagcgatga ggagtcaagc
     1861 agactgctgg ggccagatga gcggctcctg gaaaaggacg acagcgtgat tgtggaagat
     1921 tcattgtctg agcccctggg ctactgtgat gggagtgggc cagagcctca ctcccctggg
     1981 ggcatccggc tgcccaatgg caagctcaag tgtgacgtct gcggcatggt ctgtattgga
     2041 cccaacgtgc tcatggtgca caagcgcagt cacactggtg aaaggccctt ccattgcaac
     2101 cagtgtggtg cctccttcac ccagaagggg aacctgctgc gccacatcaa gctgcactct
     2161 ggggagaagc cctttaaatg tcccttctgc aactatgcct gccgccggcg tgatgcactc
     2221 actggtcacc tccgcacaca ctcagtctcc tctcccacag tgggcaagcc ctacaagtgt
     2281 aactactgtg gccggagcta caaacagcag agtaccctgg aggagcacaa ggagcggtgc
     2341 cataactacc tacagagtct cagcactgaa gcccaagctt tggctggcca accaggtgac
     2401 gaaatacgtg acctggagat ggtgccagac tccatgctgc actcatcctc tgagcggcca
     2461 actttcatcg atcgtctggc caatagcctc accaaacgca agcgttccac accccagaag
     2521 tttgtaggcg aaaagcagat gcgcttcagc ctctcagacc tcccctatga tgtgaactcg
     2581 ggtggctatg aaaaggatgt ggagttggtg gcacaccaca gcctagagcc tggctttgga
     2641 agttccctgg cctttgtggg tgcagagcat ctgcgtcccc tccgccttcc acccaccaat
     2701 tgcatctcag aactcacgcc tgtcatcagc tctgtctaca cccagatgca gcccctccct
     2761 ggtcgactgg agcttccagg atcccgagaa gcaggtgagg gacctgagga cctggctgat
     2821 ggaggtcccc tcctctaccg gccccgaggc cccctgactg accctggggc atcccccagc
     2881 aatggctgcc aggactccac agacacagaa agcaaccacg aagatcgggt tgcgggggtg
     2941 gtatccctcc ctcagggtcc cccaccccag ccacctccca ccattgtggt gggccggcac
     3001 agtcctgcct acgccaaaga ggaccccaag ccacaggagg ggttattgcg gggcacccca
     3061 ggcccctcca aggaagtgct tcgggtggtg ggcgagagtg gtgagcctgt gaaggccttc
     3121 aagtgtgagc actgccgtat cctcttcctg gaccacgtca tgttcactat ccacatgggc
     3181 tgccatggct tcagagaccc ttttgagtgc aacatctgtg gttatcacag ccaggaccgg
     3241 tacgaattct cttcccacat tgtccggggg gagcataagg tgggctaccc aactttcttg
     3301 tacaaagtgg tgggtggagg cggttcaggt ggcggaggaa gcggaggcgg tggaagttac
     3361 ccctacgacg tgcccgacta cgccggctat ccgtatgatg tcccggacta tgcaggatcc
     3421 tatccatacg acgttccaga ttacgcttag ttaattaagg ccggccctcg aggttaacga
     3481 attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc
     3541 cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac
     3601 cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc
     3661 cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga
     3721 agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt
     3781 aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct
     3841 gggaaggggt gggtccgggg gcgggctcag gggcgggctc rggggcgggg cgggcgcccg
     3901 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     3961 ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac
     4021 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc
     4081 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag
     4141 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     4201 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     4261 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     4321 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     4381 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     4441 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     4501 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     4561 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgatg tacaagtagg
     4621 aattcgtcga gggacctaat aacttcgtat agcatacatt atacgaagtt atacatgttt
     4681 aagggttccg gttccactag gtacaattcg atatcaagct tatcgataat caacctctgg
     4741 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     4801 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     4861 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     4921 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     4981 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5041 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     5101 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     5161 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     5221 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     5281 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga
     5341 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5401 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     5461 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5521 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     5581 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     5641 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     5701 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     5761 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     5821 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5881 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5941 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     6001 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6061 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6121 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattagcatg tgagcaaaag
     6181 gccagcataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     6241 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     6301 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     6361 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     6421 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     6481 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     6541 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6661 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     6721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6781 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6841 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6901 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6961 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7021 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     7081 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     7141 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     7201 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     7261 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     7321 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     7381 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     7441 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     7501 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     7561 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     7621 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     7681 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     7741 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     7801 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     7861 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     7921 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7981 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     8041 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     8101 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     8161 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     8221 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     8281 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     8341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     8401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     9001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     9061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     9121 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca tgcattagtt
     9181 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     9241 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     9301 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     9361 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     9421 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     9481 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     9541 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     9601 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     9661 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     9721 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
     9781 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9841 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9901 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9961 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
    10021 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
    10081 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
    10141 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
    10201 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
    10261 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
    10321 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
    10381 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    10441 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
    10501 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
    10561 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
    10621 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
    10681 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
    10741 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
    10801 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    10861 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    10921 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    10981 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    11041 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    11101 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    11161 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    11221 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    11281 agacagagac agatccattc gattagtgaa cggatcggca ctgcgtg
//
