LOCUS       sequence-210557-        6629 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Trc1O promoter express gene in Synechocystis 6803.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6629)
  TITLE     Synechocystis 6803 expression vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSCBe
FEATURES             Location/Qualifiers
     source          1..6629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3798..3989
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(3983..4005)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    4091..4231
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     4146..4165
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4246..4263)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4417..5005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4497..4516)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5285..6076)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(6294..6299,6300..6316,6317..6323)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    6331..6347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6365..6376
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             6386..6409
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6473..6490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      6508..6579
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6595..6622
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 aaacagcgtg acagcgaccg atctaatccg ttacaattct ggcaatagtc caagatttcc
       61 aacaaaaaaa agcttttcag gagggaatta agattgctgc agtaaaaaac gtaagaagtt
      121 tagttgacgc taaaaaactt acctacagac aataacccgg cccaaaaagc caacaaaata
      181 cttcaaaaat attgtctcta ctgtagctct aaaaattccc aaaagaaaag cggtcaactc
      241 ttgaacccga gaccgctatg aacaattata gtctttccca ccaaaattct caagccttag
      301 cccatcagca tttcaacgga tggcaagggt caaaaaatga cccaaaaata cggtcagaat
      361 ttgtagaaga ttcggcgatc gcccctgacg tattccgggc caacgtggag attgtgccgg
      421 atcttgagat agaccccatc accaaggaag tcttggcaac gccgatcgcc gatgccctgg
      481 ggcgaaaatt tacccgcttt ggacatcaag ccaaggaaga caggcaggcc gccattttcc
      541 ggtctgagaa tgggaacgta tggcaggtaa aaatctttgg tgaagacaag acgggcaagc
      601 ggtcagggca gtatctagca ccgacaggca taggggacgt gccctatctg ccaacaattc
      661 cccgccggat cattttggcg atcgccgaaa agcatggggt caaaccaccg gaagatggtc
      721 aagatttttg gccctggttt gtggatcatc ctgagattcc cttgattgtg acggaagggg
      781 ggaagaaagc tttagccgcc atcagccaag gttacgtggc cctgagtctc tatggctgtt
      841 tatgtggcaa cgatggtcta accatcaaac cgtcattatt gccctatgtg caggggcgag
      901 aagtagcgat cgcctatgac caggatgcaa agggtagcaa gggacgaaag gcagtcttta
      961 agggcacaaa acggctggct cgtaacctga cctatcacgc taaggcaacg gtcaagatct
     1021 gccaatggaa cggggaagac ggcaagggca ttgatgactt gatcgccaat aaccctgcta
     1081 aatttcaccg ggcgatcgct cggggcatca gcctagaaca gttccaaaac aagccgtatg
     1141 ctgacctatc ccaatgggtg aatcaaaccc ggcacgaaag atacctaagc aacattaatg
     1201 cccctaccac cgccaaaata atcgggatta aatcagccaa gggcacggga aagacggaag
     1261 cgatctcaaa aatggtggag caacggcgag aagattttcg ccggacttat ccactcactc
     1321 accgaattca actaggcatt gccctatccg gccggtttgg tgtggactac atcagagatt
     1381 tgtccgagag cgacactaat ggggccttag gaatcggatt gtgcattgat agcatcctga
     1441 aacttgaccc cgaaacattc cggggccatg acatcatcct tgatgaatgc gagcaattga
     1501 tatgggaatt attagatagt tcccggggaa acctgggcaa acataaacca gccatcttgg
     1561 cccgcttcca agaaatattg atcgccgccg ctgattcggg gggcaagatt attttgtctg
     1621 atgctgattt gtccccggtg actatcaagt atgtccaaga cttaatcggt ggtaaggcag
     1681 aatgctatat tgtcgttaac acgtttaagc cagtgcagga taagcgtaat ttgattgttt
     1741 ataattcccc ggaagatttg cttacttccg ctattaatac tgttgctaat ggtaaaaaaa
     1801 tacagatttg caccggggca caaaaagcac agtctatcta ttcaacaact aacctagggg
     1861 acttattcaa aatgttattc cctgataagt cagtaggggt cttagatgcc catacagtaa
     1921 gcgataagac taaccctgcc tatggctgta tggataactt agatagatat ttagaagaca
     1981 aggatattgt catcacgtcc ccggtaatag agacgggaat cagcattgat aagccctatt
     2041 ttgatgaagt ctatggattc ggacagggaa cacaaacagt agagcaattc tgccaaggat
     2101 tagaaaggta tcgtcccgat gtggatcgcc atgtttggat tcgcaatcgt gcccctaata
     2161 attgttttat tgctggcggg gaaactaacc ctcgctatat ccttaatgtg gagaataaga
     2221 aagccaaggc tactatcggg gcattgaata cagccgacaa tttaaccctg tttgatgatg
     2281 agaagccaga gcatcttaaa acctgggcta ttatggcggc tctgcacaat caagggaaga
     2341 aaagcttaaa atcaactact cttgaaaaat taggggaaga agggtacaca cagaaagaac
     2401 ctgacccaga tatggtcacc ggggcggaag acgtagctga tcgcattaaa gctatccgtg
     2461 accataacta tggaaaggaa accacggcga tcgccgagag ccttaatccc gatgacttca
     2521 cctatgaaag tttgaagcgg aagcaagtta agacggaagc ggaacgatat caagagcgga
     2581 agggagatct ttgccgtgcc ctggctaccg atgacataac cccagaagat gtggagaaat
     2641 gtgatcaagg atggctggcc caattgaccc tgcattatta cttcaccacc ggtgcagaat
     2701 ttttacaggc aagggacagc caaaggctcc aaaatttggc cggggaagat ggaaaggttt
     2761 gtgccaagga tgctagccgg aagactctca ccgtccccgt tcacttcctt aaagtattgg
     2821 gctttgaaag attccttacc gggatagggg aattttccaa ggattcccta gccaattggt
     2881 ttgagaagac tatcctgccc aacaaggcag aaattaagca ggccttgggc atcacgatca
     2941 accctgacaa aggggcgatc gccttcgtgc aatctgtctt gaaaaaaatg ggtttacatc
     3001 ttgagtatct gggccggttt ggggaacggg gcaatactaa gcgacattat ggccccgcca
     3061 atattgaccc cgatgggcga tcggctattt tttcccggtg gtttgagcgg gattctattt
     3121 tgtatcactc tgataccgta tccactgaat ccttattaat taatcaagcc taggaactgg
     3181 ataccaaaaa cagggagtta tgatgggaat ataatcccgt taacaggcta aacccatgca
     3241 ggcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc
     3301 ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     3361 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     3421 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     3481 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     3541 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     3601 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     3661 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     3721 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     3781 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     3841 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     3901 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     3961 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4021 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4081 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     4141 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     4201 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     4261 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     4321 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     4381 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     4441 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     4501 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     4561 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     4621 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     4681 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     4741 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     4801 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     4861 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     4921 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     4981 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     5041 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     5101 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     5161 aacttggtct gacagacttg acctgatagt ttggctgtga gcaattatgt gcttagtgca
     5221 tctaacgctt gagttaagcc gcgccgcgaa gcggcgtcgg cttgaacgaa tttctagcta
     5281 gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca aattcttcca
     5341 actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc tgtctagctt
     5401 caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg gcagcgacat
     5461 ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac gtaagcacta
     5521 catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag gtttcattta
     5581 gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc gctggaccta
     5641 ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca atgtcgatcg
     5701 tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca aattgcagtt
     5761 cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg gtgacttcta
     5821 cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg tcgttgatca
     5881 aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa tcaatatcac
     5941 tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc agcaacgtcg
     6001 gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat acttcggcga
     6061 tcaccgcttc ccccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat
     6121 cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg
     6181 cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa accgccactg
     6241 cgccgttacc accgctgcgt tcggtcaagg ttctggacca gttgcgtgac gcgttgacaa
     6301 ttaatcatcc ggctcgtata atgtgtggaa ttgtgagcgg ataacaattt cacacattct
     6361 agagaaagag gagaaatact agatggacta caaagacgat gacgacaagc ttgcggccgc
     6421 cgcggcatcg atagatctga gctcggtacc agtcgactcg agaggatccc ggcaccatca
     6481 ccatcaccat taactagtgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     6541 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     6601 ccttcgggtg ggcctttctg cgtttatat
//
