LOCUS       sequence-210653-        6421 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 400 / 490, Emission: 468 
            / 511.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6421)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PS-CFP2-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..6421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2424..3140
                     /codon_start=1
                     /product="photoswitchable cyan-to-green fluorescent 
                     protein"
                     /label=PS-CFP2
                     /note="mammalian codon-optimized"
                     /translation="MSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVATLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDDG
                     NYKTRAEVKFEGDTLVSRIELTGTDFKEDGNILGNKMEYNYNATNVYIVADKARNGIKV
                     NFKVRHNIKDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYLE
                     FVTAAAITHGMDELYK"
     polyA_signal    3263..3384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3300..3319)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3354..3373
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3391..3846)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3528..3549)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3740..3759
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3873..3977
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3979..4336
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3979..4170
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4002..4022)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4187..4322
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4371..5165
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4371..5165
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4425..4444)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5035..5054
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5353..5372)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5397..5444
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5773..6361
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6262..6281
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc gctaccggac
      661 tcagatctcg agctcaagct tcgaattcca tggcggcccc ccgcccgtct cccgcgatcc
      721 ccgtttcggt ctcggctccg gctttttacg ccccgcagaa gaagttcggc cctgtggtgg
      781 ccccaaagcc caaagtgaat cccttccggc ccagggacag cgagcctccc ccggcacccg
      841 gggcccagcg cgcacagatg ggccgggtgg gcgatattcc cccgccgccc ccggaagact
      901 ttcccctgcc tccacctccc cttgctgggg atggcgacga tgcagagggt gctctgggag
      961 gtgccttccc gccgccccct cccccgatcg aggaatcatt tccccctgcg cctctggagg
     1021 aggagatctt cccttccccg ccgcctcctc cggaggagga gggagggcct gaggccccca
     1081 taccgccccc accacagccc agggagaagg tgagcagtat tgatttggag atcgactctc
     1141 tgtcctcact gctggatgac atgaccaaga atgatccttt caaagcccgg gtgtcatctg
     1201 gatatgtgcc cccaccagtg gccactccat tcagttccaa gtccagtacc aagcctgcag
     1261 ccgggggcac agcacccctg cctccttgga agtccccttc cagctcccag cctctgcccc
     1321 aggttccggc tccggctcag agccagacac agttccatgt tcagccccag ccccagccca
     1381 agcctcaggt ccaactccat gtccagtccc agacccagcc tgtgtctttg gctaacaccc
     1441 agccccgagg gcccccagcc tcatctccgg ctccagcccc taagttttct ccagtgactc
     1501 ctaagtttac tcctgtggct tccaagttca gtcctggagc cccaggtgga tctgggtcac
     1561 aaccaaatca aaaattgggg caccccgaag ctctttctgc tggcacaggc tcccctcaac
     1621 ctcccagctt cacctatgcc cagcagaggg agaagccccg agtgcaggag aagcagcacc
     1681 ccgtgccccc accggctcag aaccaaaacc aggtgcgctc ccctggggcc ccagggcccc
     1741 tgactctgaa ggaggtggag gagctggagc agctgaccca gcagctaatg caggacatgg
     1801 agcatcctca gaggcagaat gtggctgtca acgaactctg cggccgatgc catcaacccc
     1861 tggcccgggc gcagccagcc gtccgcgctc tagggcagct gttccacatc gcctgcttca
     1921 cctgccacca gtgtgcgcag cagctccagg gccagcagtt ctacagtctg gagggggcgc
     1981 cgtactgcga gggctgttac actgacaccc tggagaagtg taacacctgc ggggagccca
     2041 tcactgaccg catgctgagg gccacgggca aggcctatca cccgcactgc ttcacctgtg
     2101 tggtctgcgc ccgccccctg gagggcacct ccttcatcgt ggaccaggcc aaccggcccc
     2161 actgtgtccc cgactaccac aagcagtacg ccccgaggtg ctccgtctgc tctgagccca
     2221 tcatgcctga gcctggccga gatgagactg tgcgagtggt cgccctggac aagaacttcc
     2281 acatgaagtg ttacaagtgt gaggactgcg ggaagcccct gtcgattgag gcagatgaca
     2341 atggctgctt ccccctggac ggtcacgtgc tctgtcggaa gtgccacact gctagagccc
     2401 agacggatcc accggtcgcc accatgagca agggcgccga gctgttcacc ggcatcgtgc
     2461 ccatcctgat cgagctgaat ggcgatgtga atggccacaa gttcagcgtg agcggcgagg
     2521 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     2581 tgcctgtgcc ctggcccacc ctggtggcca ccctgagcta cggcgtgcag tgcttctcac
     2641 gctaccccga tcacatgaag cagcacgact tcttcaagag cgccatgcct gagggctaca
     2701 tccaggagcg caccatcttc ttcgaggatg acggcaacta caagacgcgc gccgaggtga
     2761 agttcgaggg cgataccctg gtgagtcgca tcgagctgac cggcactgat ttcaaggagg
     2821 atggcaacat cctgggcaat aagatggagt acaactacaa cgccaccaat gtgtacatcg
     2881 tggccgacaa ggccaggaat ggcatcaagg tgaacttcaa ggtccgccac aacatcaagg
     2941 atggcagcgt gcagctggcc gaccactacc agcagaatac ccccatcggc gatggccctg
     3001 tgctgctgcc cgataaccac tacctgtcca cccagagcgc cctgtccaag gaccccaacg
     3061 agaagcgcga tcacatgatc tacctcgagt tcgtgaccgc cgccgccatc acccacggca
     3121 tggatgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
     3181 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3241 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3301 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3361 gtccaaactc atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat
     3421 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     3481 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     3541 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     3601 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     3661 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     3721 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     3781 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     3841 gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3901 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3961 attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     4021 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4081 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4141 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     4201 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     4261 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4321 cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac
     4381 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     4441 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     4501 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg
     4561 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     4621 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     4681 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4741 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4801 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4861 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc
     4921 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4981 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5041 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5101 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5161 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     5221 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     5281 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag
     5341 ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc
     5401 aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg
     5461 gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg
     5521 cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc
     5581 caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga
     5641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6421 g
//
