LOCUS       sequence-210792-        5187 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Localization: Membrane, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5187)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos4a-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..5187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        115..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1218..1227
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1224..1907
                     /codon_start=1
                     /product="fixation-resistant green-to-red photoswitchable 
                     fluorescent protein (Paez-Segala et al., 2015)"
                     /label=mEos4a
                     /note="derived from mEos2"
                     /translation="MVSAIKPDMRIKLRMEGNVNGHHFVIDGDGTGKPYEGKQTMDLEV
                     KEGGPLPFAFDILTTAFHYGNRVFVKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICYA
                     RNDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALL
                     LEGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHAIEILSHDKDYNKVKLYEHAVAHSGLP
                     DNARR"
     polyA_signal    2029..2150
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2066..2085)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2120..2139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2157..2612)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2294..2315)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2506..2525
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2639..2743
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2745..3102
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2745..2936
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2768..2788)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2953..3088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3137..3931
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3137..3931
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3191..3210)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3801..3820
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4119..4138)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4163..4210
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4539..5127
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5028..5047
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc accgccacca
      661 tgtctggggg caaatacgta gactcggagg gacatctcta caccgttccc atccgggaac
      721 agggcaacat ctacaagccc aacaacaagg ccatggcaga cgagctgagc gagaagcaag
      781 tgtacgacgc gcacaccaag gagatcgacc tggtcaaccg cgaccctaaa cacctcaacg
      841 atgacgtggt caagattgac tttgaagatg tgattgcaga accagaaggg acacacagtt
      901 ttgacggcat ttggaaggcc agcttcacca ccttcactgt gacgaaatac tggttttacc
      961 gcttgctgtc tgccctcttt ggcatcccga tggcactcat ctggggcatt tacttcgcca
     1021 ttctctcttt cctgcacatc tgggcagttg taccatgcat taagagcttc ctgattgaga
     1081 ttcagtgcat cagccgtgtc tattccatct acgtccacac cgtctgtgac ccactctttg
     1141 aagctgttgg gaaaatattc agcaatgtcc gcatcaactt gcagaaagaa ataagctccg
     1201 gaggggatcc accggtcgcc accatggtga gtgcgattaa gccagacatg aggatcaaac
     1261 tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat ggtacaggca
     1321 agccttatga gggaaaacag accatggatc ttgaagtcaa agagggcgga cctctgcctt
     1381 ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc gttaaatatc
     1441 cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg tgggaacgaa
     1501 gcttgacttt cgaagacggg ggcatttgct atgccagaaa cgacataaca atggaagggg
     1561 acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat ggtccagtta
     1621 tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg cgtgatggag
     1681 tgctgacggg tgatattcat atggctttgt tgcttgaagg aaatgcccat taccgatgtg
     1741 acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc taccactttg
     1801 tggaccacgc cattgagatt ttaagccatg acaaagatta caacaaggtt aagctgtatg
     1861 agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg gccgcgactc
     1921 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1981 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     2041 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2101 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     2161 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     2221 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     2281 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     2341 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     2401 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     2461 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     2521 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     2581 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     2641 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     2701 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     2761 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     2821 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     2881 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     2941 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     3001 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     3061 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     3121 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     3181 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     3241 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     3301 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     3361 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     3421 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     3481 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     3541 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     3601 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     3661 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     3721 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     3781 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     3841 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     3901 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     3961 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     4021 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     4081 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     4141 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     4201 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     4261 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     4321 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     4381 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4441 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4501 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4561 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4621 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4681 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4741 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4801 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4861 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4921 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4981 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5041 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5101 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5161 gccttttgct ggccttttgc tcacatg
//
