LOCUS       sequence-210822-         441 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Gal-Estradiol transcription activation expression cassette marked 
            with KanR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Costa EA, Subramanian K, Nunnari J, Weissman JS
  TITLE     Defining the physiological role of SRP in protein-targeting 
            efficiency and specificity.
  JOURNAL   Science. 2018 Jan 18. pii: science.aar3607. doi: 
            10.1126/science.aar3607.
  PUBMED    29348368
REFERENCE   2  (bases 1 to 441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Jan 18. pii: science.aar3607. doi: 10.1126/science.aar3607."
COMMENT     Sequence Label: pJW1663
FEATURES             Location/Qualifiers
     source          1..441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..441
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECCYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLGMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 atgaagctac tgtcttctat cgaacaagca tgcgatattt gccgacttaa aaagctcaag
       61 tgctccaaag aaaaaccgaa gtgcgccaag tgtctgaaga acaactggga gtgttgctac
      121 tctcccaaaa ccaaaaggtc tccgctgact agggcacatc tgacagaagt ggaatcaagg
      181 ctagaaagac tggaacagct atttctactg atttttcctc gagaagacct tggcatgatt
      241 ttgaaaatgg attctttaca ggatataaaa gcattgttaa caggattatt tgtacaagat
      301 aatgtgaata aagatgccgt cacagataga ttggcttcag tggagactga tatgcctcta
      361 acattgagac agcatagaat aagtgcgaca tcatcatcgg aagagagtag taacaaaggt
      421 caaagacagt tgactgtatc g
//
