LOCUS       sequence-210894-        2611 bp    DNA     circular SYN 14-APR-2025
DEFINITION  AtU6 Promoter-LwaCas13a-gRNA Scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, 
            Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, 
            Voytas DF, Ting AY, Zhang F
  TITLE     RNA targeting with CRISPR-Cas13.
  JOURNAL   Nature. 2017 Oct 4. doi: 10.1038/nature24049.
  PUBMED    28976959
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Oct 4. doi: 10.1038/nature24049."
COMMENT     Sequence Label: pJJB308
FEATURES             Location/Qualifiers
     source          1..2611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_RNA        1321..1395
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        1462..1566
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctactaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgctcac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca cctgccaggg gacccatggc ttcgttgaac aacggaaact
      841 cgacttgcct tccgcacaat acatcatttc ttcttagctt tttttcttct tcttcgttca
      901 tacagttttt ttttgtttat cagcttacat tttcttgaac cgtagctttc gttttcttct
      961 ttttaacttt ccattcggag tttttgtatc ttgtttcata gtttgtccca ggattagaat
     1021 gattaggcat cgaaccttca agaatttgat tgaataaaac atcttcattc ttaagatatg
     1081 aagataatct tcaaaaggcc cctgggaatc tgaaagaaga gaagcaggcc catttatatg
     1141 ggaaagaaca atagtatttc ttatataggc ccatttaagt tgaaaacaat cttcaaaagt
     1201 cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag tcgaagtagt
     1261 gattgattta gactacccca aaaacgaagg ggactaaaac gggtcttcga gaagaccttt
     1321 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     1381 gcaccgagtc ggtgcttttt ttcccggtga cgcaggtgat gaaaagcctt agggtcaggt
     1441 ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt tatttttcta aatacattca
     1501 aatatgtatc cgctaatgag acaataaccc tgataaatgc ttcaattata ttgaaaaagg
     1561 aagagtatga gtatccaaca tttccgtgtc gcccttattc ccttttttgc ggccttctgc
     1621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1681 ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt
     1741 cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg
     1801 ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1861 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1921 gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1981 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2101 acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga actacttact
     2161 cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt
     2221 ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2401 ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc
     2461 ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct
     2521 cttaggcggt ccgattagcg acatagtcat caatgccttg acggcaaaat cccttaacgt
     2581 gagttttcgt tccactgagc gtcagacccc g
//
