LOCUS       sequence-211053-        3269 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Bacterial expression of FGF2phosphomimic-NanoLuc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Botero S, Chiaroni-Clarke R, Simon SM
  TITLE     Escherichia coli as a platform for the study of phosphoinositide 
            biology.
  JOURNAL   Sci Adv. 2019 Mar 27;5(3):eaat4872. doi: 10.1126/sciadv.aat4872. 
            eCollection 2019 Mar.
  PUBMED    30944849
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2019-03-27"; volume: "5(3):eaat4872. doi"; pages: " 
            10.1126/sciadv.aat4872. eCollection 2019 Mar"
COMMENT     Sequence Label: pClodANL-FGF2YE
FEATURES             Location/Qualifiers
     source          1..3269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             205..227
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1324..1342)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1410..1514
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1515..1583,1584..2375)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1733..1752)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(2531..3269)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 agcggccgca aaaaaggtac ctgccatgta tactagacct taattaacac agctaacacc
       61 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataact ttacgggcat
      121 gcataaggct cgtataatat attcagggag accacaacgg tttccctcta caaataattt
      181 tgtttaactt tgagggacaa taattttgtt taactttaag aaggagatat acattactag
      241 tatcgatggc cgcaggtagc attaccacac tgccggcatt accggaggat ggtggtagtg
      301 gcgccttccc tccgggccat tttaaagatc cgaagcgtct gtactgtaaa aacggtggtt
      361 ttttcctgcg cattcacccg gatggccgcg ttgatggtgt gcgcgagaag agcgacccgc
      421 acattaagtt acagctgcaa gccgaggagc gcggcgttgt gagcatcaaa ggcgtttgcg
      481 caaatcgcga gctggccatg aaagaagatg gtcgcctgct ggccagtaaa tgcgtgaccg
      541 acgaatgctt cttcttcgag cgcctggaaa gcaacaacta caacacatat cgcagtcgca
      601 agtacaccag ctggtacgtt gccttaaagc gcaccggcca gtataaactg ggtagcaaaa
      661 caggcccggg ccagaaagcc atcctgtttc tgccgatgag cgcaaaaagt ggtgcatcta
      721 gaggagtttt tactctggaa gattttgttg gcgattggcg tcagaccgcg ggttataatt
      781 tggatcaagt cctggaacag ggtggcgtaa gctctctgtt ccagaacctg ggtgtgagcg
      841 tgacgccgat tcagcgcatc gttctgtccg gcgagaacgg tctgaaaatt gatattcatg
      901 tgatcatccc gtacgaaggc ctgagcggtg accaaatggg tcaaatcgag aaaatcttta
      961 aagtcgtcta cccagttgac gatcaccact tcaaggttat cttgcattac ggtacgctgg
     1021 tgattgatgg tgtgaccccg aatatgattg actatttcgg ccgtccgtat gaaggcattg
     1081 ccgtttttga cggtaaaaag atcaccgtca ccggtaccct gtggaatggc aataagatta
     1141 ttgacgagcg tctgattaac ccggacggca gcctgctgtt ccgcgtgacc atcaacggtg
     1201 tcacgggttg gcgtctgtgc gagcgcatcc tggcataata agtcgacgtt aataacaggc
     1261 ctgctggtaa tcgcaggcct ttttattttt tttttttgct agcaaattct tgaagacgaa
     1321 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1381 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1441 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1501 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1561 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1621 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1681 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1741 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
     1801 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1861 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1921 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1981 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2041 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
     2101 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2161 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2221 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2281 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2341 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2401 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2461 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2521 ccaagatcta gcgctgcgga cacatacaaa gttgcccaca gattccgtgg ataagcaggg
     2581 gactaacatg tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc
     2641 cctcctgcca gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg
     2701 ctcaaccatg aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt
     2761 tccggcgggt cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct
     2821 gttccgcctt tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc
     2881 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc
     2941 ccgactgctg cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta
     3001 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     3061 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     3121 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
     3181 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
     3241 aaaaaggatc tcaagaagat cctttgatc
//
