LOCUS       sequence-211337-       10818 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Targeted DNA methylation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10818)
  AUTHORS   Lu Z, Liu Z, Mao W, Wang X, Zheng X, Chen S, Cao B, Huang S, Zhang 
            X, Zhou T, Zhang Y, Huang X, Sun Q, Li JD
  TITLE     Locus-specific DNA methylation of Mecp2 promoter leads to 
            autism-like phenotypes in mice.
  JOURNAL   Cell Death Dis. 2020 Feb 3;11(2):85. doi: 10.1038/s41419-020-2290-x.
  PUBMED    32015323
REFERENCE   2  (bases 1 to 10818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2020-02-3"; volume: "11(2):85. doi"; pages: " 
            10.1038/s41419-020-2290-x"
COMMENT     Sequence Label: pSt1374-N-NLS-DNMT3L-L-DNMT3A-L-dcas9-NLS
FEATURES             Location/Qualifiers
     source          1..10818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             910..930
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1585..1602
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6619..6639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6682..6723
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6733..6750
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6773..6790)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6779..7003
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7049..7477
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7136..7155)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7346..7367
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7486..7506)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7491..7821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7672..7807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7734..7753
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7869..7916
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7935..8333
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8491..8612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8528..8547)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8582..8601
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8661..8677)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8661..8677)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8674..8696)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8685..8701
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8709..8715,8716..8733,8734..8739))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8754..8775
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8892..8909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9063..9651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9143..9162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9822..10613,10614..10682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10445..10464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10683..10787)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 accatgggac ctaagaaaaa gaggaaggtg gcggccgctg gcggcagcat gttcgaaacc
      961 gtgcctgtgt ggaggagaca gccagtccgg gtgctgtccc tttttgaaga catcaagaaa
     1021 gagctgacga gtttgggctt tttggaaagt ggttctgacc cgggacaact gaagcatgtg
     1081 gttgatgtca cagacacagt gaggaaggat gtggaggagt ggggaccctt cgatcttgtg
     1141 tacggcgcca cacctcccct gggccacacc tgtgaccgtc ctcccagctg gtacctgttc
     1201 cagttccacc ggctcctgca gtacgcacgg cccaagccag gcagccccag gcccttcttc
     1261 tggatgttcg tggacaatct ggtgctgaac aaggaagacc tggacgtcgc atctcgcttc
     1321 ctggagatgg agccagtcac catcccagat gtccacggcg gatccttgca gaatgctgtc
     1381 cgcgtgtgga gcaacatccc agccataagg agcagcaggc actgggctct ggtttcggaa
     1441 gaagaattgt ccctgctggc ccagaacaag cagagctcga agctcgcggc caagtggccc
     1501 accaagctgg tgaagaactg ctttctcccc ctaagagaat atttcaagta tttttcaagc
     1561 agcgggcgca gcttcagcag cggactagtg ccccgcggca gccacccagc tgagaagagg
     1621 aagcccatcc gggtgctgtc tctctttgat ggaatcgcta cagggctcct ggtgctgaag
     1681 gacttgggca ttcaggtgga ccgctacatt gcctcggagg tgtgtgagga ctccatcacg
     1741 gtgggcatgg tgcggcacca ggggaagatc atgtacgtcg gggacgtccg cagcgtcaca
     1801 cagaagcata tccaggagtg gggcccattc gatctggtga ttgggggcag tccctgcaat
     1861 gacctctcca tcgtcaaccc tgctcgcaag ggcctctacg agggcactgg ccggctcttc
     1921 tttgagttct accgcctcct gcatgatgcg cggcccaagg agggagatga tcgccccttc
     1981 ttctggctct ttgagaatgt ggtggccatg ggcgttagtg acaagaggga catctcgcga
     2041 tttctcgagt ccaaccctgt gatgattgat gccaaagaag tgtcagctgc acacagggcc
     2101 cgctacttct ggggtaacct tcccggtatg aacaggccgt tggcatccac tgtgaatgat
     2161 aagctggagc tgcaggagtg tctggagcat ggcaggatag ccaagttcag caaagtgagg
     2221 accattacta cgaggtcaaa ctccataaag cagggcaaag accagcattt tcctgtcttc
     2281 atgaatgaga aagaggacat cttatggtgc actgaaatgg aaagggtatt tggtttccca
     2341 gtccactata ctgacgtctc caacatgagc cgcttggcga ggcagagact gctgggccgg
     2401 tcatggagcg tgccagtcat ccgccacctc ttcgctccgc tgaaggagta ttttgcgtgt
     2461 gtgagcggca gcgagacccc cggtaccagc gagagcgcca cccccgagag cgacaagaaa
     2521 tactctattg gactggctat cgggacaaac tccgttggct gggccgtcat aaccgacgag
     2581 tataaggtgc caagcaagaa attcaaggtg ctgggtaata ctgaccgcca ttcaatcaag
     2641 aagaacctga tcggagcact cctcttcgac tccggtgaaa ccgctgaagc tactcggctg
     2701 aagcggaccg caaggcggag atacacccgc cgcaagaatc ggatatgtta tctgcaagag
     2761 atctttagca acgaaatggc taaggtggac gactccttct ttcaccgcct ggaagagagc
     2821 tttctggtgg aggaggataa gaaacacgag aggcacccta tattcggaaa tatcgtggat
     2881 gaggtggctt accatgaaaa gtatcctaca atctaccatc tgaggaagaa gctggtggac
     2941 agcaccgata aagcagacct gaggctcatc tatctggccc tggctcatat gataaagttt
     3001 agaggacact ttctgatcga gggcgacctg aatcccgata attccgatgt ggataaactc
     3061 ttcattcaac tggtgcagac atataaccaa ctgttcgagg agaatcccat aaacgcttct
     3121 ggtgtggatg ccaaggctat tctgtccgct cggctgtcca agtcacgcag actggagaat
     3181 ctgattgccc aactgccagg agaaaagaag aacggcctgt ttgggaacct catcgccctg
     3241 agcctgggcc tgacacctaa cttcaagtcc aattttgatc tggccgaaga tgctaaactc
     3301 cagctctcca aggacaccta tgacgatgat ctggacaacc tgctcgcaca gataggcgac
     3361 cagtacgccg atctctttct ggctgctaag aatctctccg acgccattct gctgagcgac
     3421 atactccggg tcaacactga gatcaccaaa gcacctctga gcgcctccat gataaaacgc
     3481 tatgatgaac accatcaaga cctgactctg ctcaaagccc tcgtgaggca acagctgcca
     3541 gagaagtaca aagagatatt cttcgaccag agcaagaatg gatatgccgg atacatcgat
     3601 ggcggagcat cacaggaaga attttacaag ttcatcaaac caatcctcga gaagatggac
     3661 ggtactgaag agctgctggt gaagctgaac agggaggacc tgctgaggaa gcagaggacc
     3721 tttgataatg gctccattcc acatcagata cacctgggag agctgcatgc aatcctccgc
     3781 aggcaggagg atttctatcc tttcctgaag gataaccggg agaagataga gaagatcctg
     3841 accttcagga tcccttatta cgtcggccct ctggctagag gcaactcccg cttcgcttgg
     3901 atgaccagga aatctgagga gacaattact ccttggaact tcgaagaggt cgtggataag
     3961 ggcgcaagcg cccagtcatt catcgaacgg atgaccaatt tcgataagaa cctgccaaac
     4021 gagaaggtcc tgcccaaaca ttcactcctg tacgagtatt tcaccgtcta taacgagctg
     4081 actaaagtga agtacgtgac cgagggcatg aggaagcctg ccttcctgtc cggagagcag
     4141 aagaaggcta tcgttgatct gctcttcaag actaatagaa aggtgacagt gaagcagctc
     4201 aaggaggatt actttaagaa gatcgaatgc tttgactcag tggaaatctc tggcgtggag
     4261 gaccgcttta atgccagcct gggcacttac catgatctgc tgaagataat caaagacaaa
     4321 gatttcctcg ataatgagga gaacgaggac atcctggaag atatcgtgct gaccctgact
     4381 ctgttcgagg atagagagat gatcgaagag cgcctgaaga cctatgccca tctgtttgac
     4441 gataaagtca tgaaacagct caagcggcgg cgctacactg ggtggggtag actctccagg
     4501 aaactcataa acggcatccg cgacaaacag agcggaaaga ccatcctgga tttcctgaaa
     4561 tccgacggat tcgctaacag gaacttcatg caactgattc acgatgactc tctgacattt
     4621 aaagaggaca tccagaaggc acaggtgagc ggtcaaggcg acagcctgca cgagcacatc
     4681 gccaacctcg ctggatcacc cgccataaag aagggaatac tgcagacagt caaggtcgtg
     4741 gacgaactcg tcaaagtgat gggtcggcac aagccagaga atatcgttat cgaaatggca
     4801 agggagaacc aaaccaccca gaagggccag aagaactctc gggaacggat gaaaagaatc
     4861 gaagagggaa ttaaggagct gggatctcag atactgaagg agcaccctgt ggagaataca
     4921 cagctccaga acgagaaact ctacctgtac tacctccaga acgggcggga catgtacgtt
     4981 gaccaggaac tcgacatcaa ccggctgtcc gattatgacg tggacgctat tgttccacag
     5041 tccttcctca aagatgactc cattgacaac aaggtgctga ccagatccga taaggcccgc
     5101 ggtaagtctg acaatgttcc atcagaagag gtggtcaaga agatgaagaa ttactggcgg
     5161 cagctcctca acgccaaact gatcacccag cggaagtttg acaatctgac taaggcagaa
     5221 agaggaggtc tgagcgaact cgacaaggcc ggctttatta agaggcaact ggtcgaaaca
     5281 cgccagatta ccaaacacgt ggcacaaatc ctcgactcta ggatgaacac taagtacgat
     5341 gagaacgata agctgatcag ggaagtgaaa gtgataactc tgaagagcaa gctggtgtct
     5401 gacttccgga aggactttca attctacaaa gttcgcgaaa taaacaatta ccatcatgct
     5461 cacgatgcct atctcaatgc tgtcgttggc accgccctga tcaagaaata ccctaaactg
     5521 gagtctgagt tcgtgtacgg tgactataaa gtctacgatg tgaggaagat gatagcaaag
     5581 tctgagcaag agattggcaa agccaccgcc aagtacttct tctactctaa tatcatgaat
     5641 ttctttaaga ctgagataac cctggctaac ggcgaaatcc ggaagcgccc actgatcgaa
     5701 acaaacggag aaacaggaga aatcgtgtgg gataaaggca gggacttcgc aactgtgcgg
     5761 aaggtgctgt ccatgccaca agtcaatatc gtgaagaaga ccgaagtgca gaccggcgga
     5821 ttctcaaagg agagcatcct gccaaagcgg aactctgaca agctgatcgc caggaagaaa
     5881 gattgggacc caaagaagta tggcggtttc gattccccta cagtggctta ttccgttctg
     5941 gtcgtggcaa aagtggagaa aggcaagtcc aagaaactca agtctgttaa ggagctgctc
     6001 ggaattacta ttatggagag atccagcttc gagaagaatc caatcgattt cctggaagct
     6061 aagggctata aagaagtgaa gaaagatctc atcatcaaac tgcccaagta ctctctcttt
     6121 gagctggaga atggtaggaa gcggatgctg gcctccgccg gagagctgca gaaaggaaac
     6181 gagctggctc tgccctccaa atacgtgaac ttcctgtatc tggcctccca ctacgagaaa
     6241 ctcaaaggta gccctgaaga caatgagcag aagcaactct ttgttgagca acataaacac
     6301 tacctggacg aaatcattga acagattagc gagttcagca agcgggttat tctggccgat
     6361 gcaaacctcg ataaagtgct gagcgcatat aataagcaca gggacaagcc aattcgcgaa
     6421 caagcagaga atattatcca cctctttact ctgactaatc tgggcgctcc tgctgccttc
     6481 aagtatttcg atacaactat tgacaggaag cggtacacct ctaccaaaga agttctcgat
     6541 gccaccctga tacaccagtc aattaccgga ctgtacgaga ctcgcatcga cctgtctcag
     6601 ctcggcggcg acggttctcc caagaagaag aggaaagtct cgagcggtgg agctgcagga
     6661 taggaattcg ggcccttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     6721 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     6781 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     6841 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     6901 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     6961 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     7021 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     7081 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     7141 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     7201 cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     7261 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     7321 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     7381 cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc ctattggtta
     7441 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     7501 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc
     7561 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7621 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7741 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     7801 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     7861 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     7921 tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     7981 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     8041 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     8101 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     8161 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgtcgacagg
     8221 tgcttctcga tctgcatcct gggatcaaag cgatagtgaa ggacagtgat ggacagccga
     8281 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taagcacttc
     8341 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     8401 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     8461 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     8521 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8581 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     8641 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8701 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8761 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8821 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     8881 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     8941 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9001 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9061 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9121 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9181 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9241 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9301 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     9361 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     9421 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     9481 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     9541 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     9601 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     9661 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     9721 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     9781 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     9841 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     9901 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     9961 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10021 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10081 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10141 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10201 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10261 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10321 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    10381 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    10441 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    10501 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    10561 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    10621 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    10681 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    10741 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    10801 aaagtgccac ctgacgtc
//
