LOCUS       sequence-211534-        9985 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fibroblasts specific inducible Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9985)
  TITLE     Cre-inducible plasmids
REFERENCE   2  (bases 1 to 9985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: Col1A2:TFPnls-T2a-mErt-Cre-Ert
FEATURES             Location/Qualifiers
     source          1..9985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(29..457)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(139..160)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     351..370
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     584..606
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     598..615
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     599..615
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     648..667
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        648..666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2452..2468)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2453..2469)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     intron          2940..3072
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3055..3074
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     3169..3188
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      3236..3245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3242..3949
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             3974..3994
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3998..4018
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4022..4042
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4064..4117
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5072..6094
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6107..7042
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6155..7048
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(7132..7151)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7197..7252
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7251..7270)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7773..7793)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7773..7791)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7835..7851)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7835..7851)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7848..7870)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7859..7875
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7883..7889,7890..7907,7908..7913))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7928..7949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8066..8083)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8237..8825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8317..8336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8996..9787,9788..9856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9619..9638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9857..9961)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
       61 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
      121 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
      181 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
      241 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
      301 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
      361 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
      421 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc
      481 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
      541 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
      601 aaaacgacgg ccagtgagcg cgtagggata acagggtaat gcgcgcgtaa tacgactcac
      661 tatagggcga attgggtacc cagaggtgta aagtacttga gtaattttac ttgattactg
      721 tacttaagta ttatttttgg ggatttttac tttacttgag tacaattaaa aatcaatact
      781 tttactttta cttaattaca tttttttaga aaaaaaagta ctttttactc cttacaattt
      841 tatttacagt caaaaagtac ttattttttg gagatcactt gggcccggct cgagcatgca
      901 cgcgtgaatt ccccaatttt tctctgtgaa tctcctccca cacacacacc ctttttctcc
      961 tgggcttcag catccatcgt aactagttag ctccactcac attatccact attttactca
     1021 aaatgcgagg attactcttt gctttatctt caagagaagt ggagagagcc agacaaagga
     1081 agaagaggga gaacaggaga ggagatgagg ggggagatta gaatggggta agggagagaa
     1141 caggagctga gaagagcggg agagggggag agaaggattc agacattcta aactctcttc
     1201 ctgagtgcta tttaaagcag tttccaacct ggagagaatg taatgcacag ttcctccatg
     1261 tgtttagtta aggacctcat gagtcttgtg gatttctcca caacctaagt ggttattatg
     1321 ctgacccgag ggtgtgccat ttggtgggac acttcatctt attgtccctg tttatgtgta
     1381 gcaagcaagt tcagttctgt aagacactga ttagaggggc aggaaaattg ttctctactg
     1441 tgccttgtgt acagcaagaa gctgtccagt gttgttcaca taattgaatg tgtggaactt
     1501 cccagcttgc aggtggggtc ttcagttcta cctcagtctc tttgggagac taccacaact
     1561 caccttgagt aattagaaaa cagcacagaa gtggctgcgc ttcctgaaga ctacaaagga
     1621 gctgttgcct gcttctctta actccatctt gggctcagct gacccaaaag cggctgtctt
     1681 tgtttacacc agatttcaac tttgcaggca atcttactaa gcagagggag gcagtggggg
     1741 atggtattgg gggtgagtta cttagtccaa gtcccagagc tgagggtgag ggagatgctg
     1801 tactttctta ccagctgtta tgcatcaaga agaggcaggc ttgtgtaacc ctttcttctc
     1861 ctgcacgttt caagcaaaag ccaggcactc actgagtttt gtaaagattt tcccgtgaag
     1921 acatacacac agcgtaattc catgcaataa tttctgtgta aagcccttag tgcagtgtac
     1981 cattgtttgt gtctgggtct tgaattgtca caattaagtg tgagcacaca cacaatgtgc
     2041 tctttagcca aagtcataac actgctacct gcttcccttt gaaagaaaac agccatcttc
     2101 tcaggaaatg taagggggca aggggtgggg gttaggggga aggacaagat tgtaaaaagc
     2161 caaaagccta gggcttggag ctgctttggc caagcctaga atctatttaa atgcccttcc
     2221 ctgaagggtt aagtgtggaa cacctcccag gaaatgacaa atggccagag ttctgcccac
     2281 tcccttcccc ccatcttcca gacatcaaat acttaggaac tgtttggctt gaaatggttc
     2341 ctgagactca tactcacaga acacattctt tttactctcc tcccccagcc cagtacaggc
     2401 tggaggtggc tggcgggggt agggggggat gggtgaaggg ggcaagctta tcgataccgt
     2461 cgacctcgag gttctagagc ttggatctgt aaagagccca cgtaggtgtc ctaaagtgct
     2521 tccaaacttg gcaagggcga gagagggcgg gtggctgggg agggcggagg tatgcagaca
     2581 gggagtcaga gttccccctc gaaagcctca aaagtgtcca cgtcctcaaa aagaatggaa
     2641 ccaatttaag aagccccgta gccacgtccc tcccccctcg gctccccccc ctgctccccc
     2701 gcagtctcct cccagcactg agtcccgggc ccctagccct agccctccca ttggtggaga
     2761 cgtttttgga ggcaccctcc ggctggggaa acttttccca tataaataag gcaggtctgg
     2821 gctttattat tttagcacca cggcagcagg aggtttcgac taagttggag ggaacggtcc
     2881 acgattgcca aggaattcga attttaagct gcagaagttg gtcgtgaggc actgggcagg
     2941 taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac
     3001 agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct
     3061 ttctctccac aggtgtccac tcccagttca attacagctc ttaaaattcc gccactagag
     3121 cctctgctaa ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg
     3181 ttattgtgct gtctcatcat tttggcaaag aattattccg ctagcgctac cggtcgccac
     3241 catggtgagc aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa
     3301 gctgaagatg gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg
     3361 caagccctac gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc
     3421 cttctcctac gacattctga ccaccgcgtt cgcctacggc aacagggcct tcaccaagta
     3481 ccccgacgac atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg
     3541 caccatgacc ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga
     3601 ggactccttc atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt
     3661 gatgcagaag aagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg
     3721 cgtgctgaag ggcgacgtca agcacaagct gctgctggag ggcggcggcc accaccgcgt
     3781 tgacttcaag accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt
     3841 ggaccaccgc atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga
     3901 gagcgccgtg gcccgcaatt ccaccgacgg catggacgag ctgtacaagt ccggactcag
     3961 atctcgagct gatccaaaaa agaagagaaa ggtagatcca aaaaagaaga gaaaggtaga
     4021 tccaaaaaag aagagaaagg taggatccac cggatctaga cccgagggca gaggaagtct
     4081 tctaacatgc ggtgacgtgg aggagaatcc cggcccacta gtcatggccg gcgatatgag
     4141 agccgctaac ctgtggccct ccccactgat gatcaagaga agcaagaaga actccctggc
     4201 cctgagcctg accgctgatc agatggtgtc cgccctgctg gacgccgagc cccctatcct
     4261 gtacagcgag tacgacccca ccaggccctt cagcgaggcc agcatgatgg gactgctgac
     4321 aaacctggcc gatcgcgaac tggtgcatat gatcaattgg gccaagcgcg tgcccggatt
     4381 cgtggacctg acactgcacg accaggtgca cctgctggaa tgcgcttggc tggaaattct
     4441 gatgatcggc ctcgtgtggc ggagcatgga acatcctgtg aagctgctgt tcgcccccaa
     4501 tctgctgctg gaccgcaacc agggcaaatg cgtggaaggg atggtcgaaa tctttgatat
     4561 gctgctcgcc accagcagca ggttcagaat gatgaacctc cagggcgaag agttcgtctg
     4621 cctgaagtcc atcatcctgc tgaacagcgg cgtgtacacc ttcctgtcct ctaccctgaa
     4681 gtccctcgag gaaaaggatc acatccacag ggtgctggat aagatcaccg ataccctgat
     4741 ccatctcatg gctaaggccg gactcacact ccagcagcag catcagagac tcgctcagct
     4801 gctgctgatc ctgagccaca tccgccacat gtccaacaag ggaatggaac acctctactc
     4861 tatgaagtgt aaaaacgtcg tccccctgta cgatctgctg ctcgaggccg ccgatgccca
     4921 cagactgcac gcccctacaa gcagaggcgg cgctagcgtg gaagagacag accagtccca
     4981 cctggccacc gccggcagca caagcagcca cagcctgcag aagtactata tcaccggcga
     5041 ggccgagggc ttccccgcta cagctgtcga caatttactg accgtacacc aaaatttgcc
     5101 tgcattaccg gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag
     5161 ggatcgccag gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg
     5221 ggcggcatgg tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg
     5281 cgattatctt ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt
     5341 gggccagcta aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc
     5401 tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa
     5461 acaggctcta gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag
     5521 cgatcgctgc caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt
     5581 acgtatagcc gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag
     5641 aatgttaatc catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc
     5701 acttagcctg ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga
     5761 tgatccgaat aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc
     5821 caccagccag ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat
     5881 ttacggcgct aaggatgact ccggtcagag atacctggcc tggtctggac acagtgcccg
     5941 tgtcggagcc gcgcgagata tggcccgcgc tggagtttca ataccggaga tcatgcaagc
     6001 tggtggctgg accaatgtaa atattgtcat gaactatatc cgtaacctgg atagtgaaac
     6061 aggggcaatg gtgcgcctgc tggaagatgg cgatctcgag ccatctgctg gagacatgag
     6121 agctgccaac ctttggccaa gcccgctcat gatcaaacgc tctaagaaga acagcctggc
     6181 cttgtccctg acggccgacc agatggtcag tgccttgttg gatgctgagc cccccatact
     6241 ctattccgag tatgatccta ccagaccctt cagtgaagct tcgatgatgg gcttactgac
     6301 caacctggca gacagggagc tggttcacat gatcaactgg gcgaagaggg tgccaggctt
     6361 tgtggatttg accctccatg atcaggtcca ccttctagaa tgtgcctggc tagagatcct
     6421 gatgattggt ctcgtctggc gctccatgga gcacccagtg aagctactgt ttgctcctaa
     6481 cttgctcttg gacaggaacc agggaaaatg tgtagagggc atggtggaga tcttcgacat
     6541 gctgctggct acatcatctc ggttccgcat gatgaatctg cagggagagg agtttgtgtg
     6601 cctcaaatct attattttgc ttaattctgg agtgtacaca tttctgtcca gcaccctgaa
     6661 gtctctggaa gagaaggacc atatccaccg agtcctggac aagatcacag acactttgat
     6721 ccacctgatg gccaaggcag gcctgaccct gcagcagcag caccagcggc tggcccagct
     6781 cctcctcatc ctctcccaca tcaggcacat gagtaacaaa ggcatggagc atctgtacag
     6841 catgaagtgc aagaacgtgg tgcccctcta tgacctgctg ctggaggcgg cggacgccca
     6901 ccgcctacat gcgcccacta gccgtggagg ggcatccgtg gaggagacgg accaaagcca
     6961 cttggccact gcgggctcta cttcatcgca ttccttgcaa aagtattaca tcacggggga
     7021 ggcagagggt ttccctgcca cagcttaaga tatcactcct caggtgcagg ctgcctatca
     7081 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     7141 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     7201 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     7261 gacatatggg agggcaaatc atttaaaaca tcagaatgag catttggttt agagtttggc
     7321 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     7381 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     7441 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     7501 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     7561 tcttctctta tgaagatccc tcgaccagcg gccgctaata ctcaagtaca attttaatgg
     7621 agtacttttt tacttttact caagtaagat tctagccaga tacttttact tttaattgag
     7681 taaaattttc cctaagtact tgtactttca cttgagtaaa atttttgagt actttttaca
     7741 cctctgccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcatt
     7801 accctgttat ccctacgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7861 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7921 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7981 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     8041 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     8101 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8161 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8221 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8281 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8341 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8401 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     8461 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8521 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8581 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8641 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     8701 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8761 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8821 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8881 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8941 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     9001 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     9061 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9121 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9181 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9241 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9301 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9361 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9421 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9481 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9541 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9601 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9661 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9721 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9781 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9841 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9901 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9961 gcacatttcc ccgaaaagtg ccacc
//
