LOCUS       sequence-211889-        5528 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Fast indicator for synaptic glutamate imaging (synapsin promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5528)
  AUTHORS   Helassa N, Durst CD, Coates C, Kerruth S, Arif U, Schulze C, Wiegert
            JS, Geeves M, Oertner TG, Torok K
  TITLE     Ultrafast glutamate sensors resolve high-frequency release at 
            Schaffer collateral synapses.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 
            10.1073/pnas.1720648115.
  PUBMED    29735711
REFERENCE   2  (bases 1 to 5528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 May 7. pii: 1720648115. doi: 
            10.1073/pnas.1720648115."
COMMENT     Sequence Label: pCI syn iGluf
FEATURES             Location/Qualifiers
     source          1..5528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        59..506
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     intron          543..675
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     658..677
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        720..738
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     829..891
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     primer_bind     1857..1878
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1984..2005)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2245..2264)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             2470..2499
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2503..2649
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    2676..2797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2687..2706)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2741..2760
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      2978..3433
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3065..3084)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3275..3296
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3500..3519)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3619..3641
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3679..3697)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3765..3869
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3870..3938,3939..4730)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4088..4107)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4901..5489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5390..5409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgctggcctt ttgctcacat ggctcgacag atctaatctg cagagggccc tgcgtatgag
       61 tgcaagtggg ttttaagacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      121 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      181 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      241 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      301 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      361 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      421 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      481 gagtcgtgtc gtgcctgaga gcgcagctgc agctaggaag ttggtcgtga ggcactgggc
      541 aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg ggcttgtcga
      601 gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac atccactttg
      661 cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc tagagtactt
      721 aatacgactc actataggct agcgctaccg gactcaagct tggtaccgaa ctcggatcca
      781 ctagtaacgg ccgccagtgt gctggaattc ggcttgggga tatccaccat ggagacagac
      841 acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga cagatctgcc
      901 gcaggcagca cgctggacaa aatcgccaaa aacggtgtga ttgtcgtcgg tcaccgtgat
      961 tcttcagtgc ctttctctta ttacgacaat cagcaaaaag tggtgggtta ctcgcaggat
     1021 tactccaacg ccattgttga agcagtgaaa aagaaactca acaaaccgga cttgcaggta
     1081 aaactgattc cgattacctc acaaaaccgt attccactgc tgcaaaacgg cactttcgat
     1141 tttgaatgtg gttctaccac caacaacgtc gaacgccaaa aacaggcggc tttctctgac
     1201 actattttcg tggtcggtac gcgcctgttg accaaaaagg gtggcgatat caaagatttt
     1261 gccaacctga aagacaaagc cgtagtcgtc acttccggca ctacctctga agttttgctc
     1321 aacaaactga atgaagagca aaaaatgaat atgcgcatca tcagcgccaa agatcacggt
     1381 gactctttcc gcaccctgga aagcggtcgt gccgttgcct ttatgatgga tgacgtgctg
     1441 ctggccggtg aacgtgcgaa agcgaagaaa ccagacaact gggaaatcgt cggcaagccg
     1501 cagtctcagg aggcctacgg ttgtatgttg cgtaaagatg atccgcagtt caaaaagctg
     1561 atggatgaca ccatcgctca ggtgcagacc tccggtgaag cggaaaaatg gtttgataag
     1621 tggttcaaaa atccaattct ggtgagccac aacgtctata tcatggccga caagcagagg
     1681 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcgg cgtgcagctc
     1741 gcctaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1801 cactacctga gcacccagtc caaactttcg aaagacccca acgagaagcg cgatcacatg
     1861 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1921 ggcggtaccg gagggagcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     1981 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt
     2041 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2101 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     2161 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacatc
     2221 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2281 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2341 ggcaacatcc tggggcataa gctggagtac aactttaaca accccctgaa catgaatttc
     2401 gaactgtcag acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaaactg
     2461 caggtcgacg aacaaaaact catctcagaa gaggatctga atgctgtggg ccaggacacg
     2521 caggaggtca tcgtggtgcc acactccttg ccctttaagg tggtggtgat ctcagccatc
     2581 ctggccctgg tggtgctcac catcatctcc cttatcatcc tcatcatgct ttggcagaag
     2641 aagccacgtt aggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     2701 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2761 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2821 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     2881 atgtggtaaa atcgataagg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
     2941 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
     3001 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     3061 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     3121 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     3181 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     3241 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     3301 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     3361 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     3421 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     3481 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     3541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     3601 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     3661 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3721 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     3961 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4021 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4081 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4141 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4201 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4261 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4321 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4381 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4441 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4501 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4561 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4621 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4681 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4741 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4801 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4861 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4921 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4981 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5041 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5101 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5161 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5221 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5281 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5341 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5401 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5461 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5521 tggccttt
//
